The JES6-5H4 antibody binds to mouse Interleukin-2 (IL-2), a 17 kDa cytokine that is secreted by activated T cells. This cytokine acts as an autocrine stimulatory factor for T cell proliferation, differentiation and survival, as well as being an effective activator of B cells and inducer of NK cell cytotoxic functions. IL-2 plays a key modulatory role in the differentiation of T cells toward either Th17 or T regulatory (Treg) cell types and is important for Treg cell function. The IL-2 receptor (IL-2R) is a complex consisting of an IL-2R alpha chain (CD25), along with the IL-2R beta chain (CD122) and the common gamma chain (CD132). IL-2 may also bind with low affinity to the T cell surface protein CD25 alone, although this interaction does not induce cell signaling.

The JES6-5H4 antibody is extensively used for intracellular detection of IL-2 by flow cytometry, for detection of soluble cytokine in ELISA, and as a neutralising reagent in vitro and in vivo.

Type: Primary
Antigen: IL2 (interleukin 2)
Clonality: monoclonal
Clone: JES6-5H4
Conjugation: Biotin
Epitope:
Host: Rat
Isotype: IgG2b Kappa
Reactivity: Mouse