Foxp3 / transcription factor staining buffer kit

Foxp3/transcription factor staining buffer contains specially formulated buffers and solutions for optimal resolution and low background in your analysis of nuclear antigens by flow cytometry. This complete kit contains three components for use in these staining protocols for detection of nuclear antigens such as Foxp3 and ROR gamma. Foxp3/transcription factor fix/perm concentrate (4x) 50 ml. A concentrated solution which, when diluted with Foxp3/transcription factor fix/perm diluent (1x) provides best results in protocols for intranuclear staining of transcription factors using fluorescently conjugated antibodies.

: Includes (1) Foxp3/transcription factor fix/Perm Concentrate (4x) 50 ml, a concentrated solution, which when diluted with Foxp3/transcription factor fix/perm diluent (1x) provides best results in protocols for intranuclear staining of transcription factors using fluorescently conjugated antibodies; (2) Foxp3/transcription factor fix/perm diluent (1x) 160 ml intended for use as a diluent for Foxp3/transcription factor fix/perm concentrate (4x); (3) flow cytometry perm buffer (10x) 150 ml provided as a concentrate, which when diluted with distilled water to a 1x solution provides best results in intracellular staining protocols for cytokines and other cytoplasmic antigens, by maintaining membrane permeabilization throughout staining and wash steps.