Affinity chromatography column, HiPrep™ Heparin FF

HiPrep Heparin FF columns are well suited for capture or intermediate purification of proteins with affinity for heparin.

• Suitable for plasma protein purification of various proteins, including antithrombin III antibodies and coagulation factors
• Prepacked with Heparin Sepharose Fast Flow resin
• Heparin ligand provides enhanced coupling chemistry and chemically stable ligand attachment
• HiPrep column format: optimized for convenient purification scale-up

Heparin resin allows for fast and reliable separations of biomolecules with an affinity for heparin, including antithrombin III antibodies, coagulation factors, and other plasma proteins. DNA-binding proteins, lipoproteins, protein synthesis factors, enzymes that act on nucleic acids, and steroid receptors can also be purified.

Heparin Sepharose 6 Fast Flow is composed of cross-linked 6% agarose beads, modified with sodium heparin. Immobilization is performed using chemically stable and oriented coupling, which delivers high binding capacity. The chemical stability of the heparin resin is solely dictated by the ligand.