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Supplier: Charles River Laboratories Cell Solutions

M1 and M 2 macrophages are derived from purified CD14⁺ monocytes. CD14⁺ monocytes were positively selected via immunomagnetic beads and were cultured in the presence of GM-CSF(M1) or M-CSF(M2) for 10 days. The phenotyping was confirmed by morphology and the expression of specific biomarkers.

Supplier: Charles River Laboratories Cell Solutions

CD19⁺ B cells are isolated from mononuclear cells by positive or negative selection using immunomagnetic cell separation procedures. Peripheral blood cells are collected from normal human volunteers by direct collection or by using the COBE® Spectra or Spectra Optia® Apheresis System where cell-rich leukapheresis product is extracted using continuous flow centrifugal technology directly into the collection bag.

Supplier: Charles River Laboratories Cell Solutions

Naive CD4⁺ T cells are isolated from mononuclear cells by positive selection using immunomagnetic cell separation procedures.

Supplier: Charles River Laboratories Cell Solutions

Peripheral blood mononuclear cells (PBMC) are isolated from blood collected in FDA-registered collection centres, from IRB consented healthy human donors and following cGMP and cGTP collection guidelines.

Supplier: Charles River Laboratories Cell Solutions

A Leukopak is an enriched leukapheresis product collected from normal peripheral blood. It is composed of a variety of blood cells including monocytes, lymphocytes, platelets, plasma, and red cells. A leukopak contains a higher concentration of cells as compared to standard venipuncture collection methods or buffy coat products.

Supplier: G-Biosciences

GenLysates™ are total protein tissue and cell lysates that are extracted from the tissue or cell of interest. To inhibit proteolysis, the GenLysates™ are prepared in a lysis buffer containing a complete protease inhibitor cocktail. Each lysate lot is tested by electrophoresis and Western blot analysis.

Supplier: Novus Biologicals

p38 Overexpression Lysate (Adult Normal)

Supplier: PROVITRO

HCASMC - Human coronary artery smooth muscle cells - are obtained from human tissue, in particularly from the coronary artery smooth muscle cells, initially isolated in their in vivo state. The cells are then transitioned into a primary cell culture and subsequently aliquoted and frozen for preservation.

Supplier: ProSci Inc.

Herzgewebelysat (7 Tage alt) wurde durch Homogenisierung in modifiziertem RIPA-Puffer (150 mM Natriumchlorid, 50 mM Tris-HCl, pH 7,4, 1 mM Ethylendiamintetraessigsäure, 1 mM Phenylmethylsulfonylfluorid, 1% Triton X-100, 1% Natriumdesoxycholsäure, 0,1% Natriumdodecylsulfat, 5 μg/ml Aprotinin, 5 μg/ml Leupeptin) hergestellt.

Supplier: ProSci Inc.

Hirngewebelysat (0 Tage alt) wurde durch Homogenisierung in modifiziertem RIPA-Puffer (150 mM Natriumchlorid, 50 mM Tris-HCl, pH 7,4, 1 mM Ethylendiamintetraessigsäure, 1 mM Phenylmethylsulfonylfluorid, 1% Triton X-100, 1% Natriumdesoxycholsäure, 0,1% Natriumdodecylsulfat, 5 μg/ml Aprotinin, 5 μg/ml Leupeptin) hergestellt.

Supplier: ProSci Inc.

Lungengewebelysat (7 Tage alt) wurde durch Homogenisierung in modifiziertem RIPA-Puffer (150 mM Natriumchlorid, 50 mM Tris-HCl, pH 7,4, 1 mM Ethylendiamintetraessigsäure, 1 mM Phenylmethylsulfonylfluorid, 1% Triton X-100, 1% Natriumdesoxycholsäure, 0,1% Natriumdodecylsulfat, 5 μg/ml Aprotinin, 5 μg/ml Leupeptin) hergestellt.

Supplier: PROVITRO

Human Adipose-Derived Stem Cells (ADSCs) are extracted from human lipoaspirate tissue obtained during elective liposuction surgeries. These cells are available from both healthy donors and patients. ADSCs exhibit phenotypic and functional characteristics similar to bone marrow-derived mesenchymal stem cells.

Supplier: PROVITRO

Bone Marrow CD34⁺ cells, enriched with CD34⁺ markers, are pivotal for haematopoiesis and immune modulation. These unique cells play a crucial role in hematopoietic stem cell transplantation and immune reconstitution. They contribute to the formation of various blood cell lineages, including immune effectors such as natural killer cells and cytotoxic T-cells, shaping both the adaptive and functional aspects of the immune system.

Supplier: Charles River Laboratories Cell Solutions

Human mononuclear cells are isolated from IRB-consented volunteer donors diagnosed with HIV or AIDS.

Supplier: Charles River Laboratories Cell Solutions

Mononuclear cells used for researching the causes, diagnosis, supportive care and treatment of Diabetes Mellitus 2 (DM2), the most common form of diabetes. Human mononuclear cells are collected from human volunteer donors diagnosed with DM2 under IRB approved informed consent by whole blood or leukapheresis using the COBE® Spectra Apheresis System MNC collection protocol.

Supplier: Charles River Laboratories Cell Solutions

Human peripheral blood CD14⁺ monocytes are isolated from peripheral blood mononuclear cells (PBMCs) by either positive or negative selection using immunomagnetic cell separation procedures. Peripheral blood cells are collected from normal human volunteers by direct collection or by using the COBE® Spectra or Spectra Optia® Apheresis System where cell-rich leukapheresis product is extracted using continuous flow centrifugal technology directly into the collection bag.

Supplier: Charles River Laboratories Cell Solutions

Naive T cells are isolated by depleting CD45RO^₊ cells via immunomagnetic bead separation from a freshly collected leukopheresis product. Naive T cells are phenotypically classified as CD45⁺, CD^₊, and CD45RA^₊.

Supplier: Charles River Laboratories Cell Solutions

CD8⁺ cytotoxic T cells are isolated from mononuclear cells by positive selection using immunomagnetic cell separation procedures.

Supplier: Charles River Laboratories Cell Solutions

CD4⁺/CD25⁺ regulatory T cells were isolated from leukapheresis product by CD127 depletion and positive selection using CD25 immunomagnetic beads. Peripheral blood cells are collected from normal human volunteers by direct collection or by using the COBE® Spectra or Spectra Optia® Apheresis System where cell-rich leukapheresis product is extracted using continuous flow centrifugal technology directly into the collection bag.

Supplier: Charles River Laboratories Cell Solutions

A Leukopak is an enriched leukapheresis product collected from normal peripheral blood. It is composed of a variety of blood cells including monocytes, lymphocytes, platelets, plasma, and red cells. A leukopak contains a higher concentration of cells as compared to standard venipuncture collection methods or buffy coat products.

Supplier: G-Biosciences

GenLysates™ are total protein tissue and cell lysates that are extracted from the tissue or cell of interest. To inhibit proteolysis, the GenLysates™ are prepared in a lysis buffer containing a complete protease inhibitor cocktail. Each lysate lot is tested by electrophoresis and Western blot analysis.

Supplier: Enzo Life Sciences

Prepared by high salt extraction of HeLa nuclei (human cervical cancer cell line), this extract is a rich source of HDAC activity, useful for inhibitor screening or immunoprecipitation of HDAC containing complexes. Typically, 0,5 µl or less per well is sufficient for assay with the HDAC FLUOR DE LYS® fluorometric activity assay.

Supplier: PROVITRO

Bone Marrow CD34⁺ cells, enriched with CD34⁺ markers, are pivotal for haematopoiesis and immune modulation. These unique cells play a crucial role in hematopoietic stem cell transplantation and immune reconstitution. They contribute to the formation of various blood cell lineages, including immune effectors such as natural killer cells and cytotoxic T-cells, shaping both the adaptive and functional aspects of the immune system.

Supplier: PROVITRO

Dental Pulp Stem Cells (DPSCs) are primarily sourced from the pulp tissues of deciduous teeth, primary incisors, and permanent third molars. The dental pulp, especially from third molars, is noted for its rich reservoir of stem cells. DPSCs derived from third molars express key pluripotency-associated transcription factors including oct4, sox2, klf4, nanog, and c-myc.

Supplier: PROVITRO

HUVEC-p - Human Umbilical Vein Endothelial Cells, pooled - are obtained from human tissue, initially isolated in their in vivo state. The cells are then transitioned into a primary cell culture and subsequently aliquoted and frozen for preservation. It's crucial to note that HUVEC-p cultures undergo careful methods during isolation, ensuring that they remain untransformed and unmutated, maintaining characteristics reflective of their in vivo state. These primary cultures have a limited in-vitro lifespan.

Supplier: ProSci Inc.

Augengewebelysat (7 Tage alt) wurde durch Homogenisierung in modifiziertem RIPA-Puffer (150 mM Natriumchlorid, 50 mM Tris-HCl, pH 7,4, 1 mM Ethylendiamintetraessigsäure, 1 mM Phenylmethylsulfonylfluorid, 1 % Triton X-100, 1 % Natriumdesoxycholsäure, 0,1 % Natriumdodecylsulfat, 5 μg/ml Aprotinin, 5 μg/ml Leupeptin) hergestellt.

Supplier: ProSci Inc.

Magengewebelysat (0 Tage alt) wurde durch Homogenisierung in modifiziertem RIPA-Puffer (150 mM Natriumchlorid, 50 mM Tris-HCl, pH 7,4, 1 mM Ethylendiamintetraessigsäure, 1 mM Phenylmethylsulfonylfluorid, 1% Triton X-100, 1% Natriumdesoxycholsäure, 0,1% Natriumdodecylsulfat, 5 μg/ml Aprotinin, 5 μg/ml Leupeptin) hergestellt.

Supplier: ProSci Inc.

Magengewebelysat (14 Tage alt) wurde durch Homogenisierung in modifiziertem RIPA-Puffer (150 mM Natriumchlorid, 50 mM Tris-HCl, pH 7,4, 1 mM Ethylendiamintetraessigsäure, 1 mM Phenylmethylsulfonylfluorid, 1 % Triton X-100, 1 % Natriumdesoxycholsäure, 0,1 % Natriumdodecylsulfat, 5 μg/ml Aprotinin, 5 μg/ml Leupeptin) hergestellt.

Supplier: ProSci Inc.

Lungengewebelysat (14 Tage alt) wurde durch Homogenisierung in modifiziertem RIPA-Puffer (150 mM Natriumchlorid, 50 mM Tris-HCl, pH 7,4, 1 mM Ethylendiamintetraessigsäure, 1 mM Phenylmethylsulfonylfluorid, 1% Triton X-100, 1% Natriumdesoxycholsäure, 0,1% Natriumdodecylsulfat, 5 μg/ml Aprotinin, 5 μg/ml Leupeptin) hergestellt.

Supplier: ProSci Inc.

Herzgewebelysat (0 Tage alt) wurde durch Homogenisierung in modifiziertem RIPA-Puffer (150 mM Natriumchlorid, 50 mM Tris-HCl, pH 7,4, 1 mM Ethylendiamintetraessigsäure, 1 mM Phenylmethylsulfonylfluorid, 1% Triton X-100, 1% Natriumdesoxycholsäure, 0,1% Natriumdodecylsulfat, 5 μg/ml Aprotinin, 5 μg/ml Leupeptin) hergestellt.