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Supplier: ProSci Inc.

Thymusgewebelysat (7 Tage alt) wurde durch Homogenisierung in modifiziertem RIPA-Puffer (150 mM Natriumchlorid, 50 mM Tris-HCl, pH 7,4, 1 mM Ethylendiamintetraessigsäure, 1 mM Phenylmethylsulfonylfluorid, 1% Triton X-100, 1% Natriumdesoxycholsäure, 0,1% Natriumdodecylsulfat, 5 μg/ml Aprotinin, 5 μg/ml Leupeptin) hergestellt.

Supplier: ProSci Inc.

Hirngewebelysat (7 Tage alt) wurde durch Homogenisierung in modifiziertem RIPA-Puffer (150 mM Natriumchlorid, 50 mM Tris-HCl, pH 7,4, 1 mM Ethylendiamintetraessigsäure, 1 mM Phenylmethylsulfonylfluorid, 1% Triton X-100, 1% Natriumdesoxycholsäure, 0,1% Natriumdodecylsulfat, 5 μg/ml Aprotinin, 5 μg/ml Leupeptin) hergestellt.

Supplier: Charles River Laboratories Cell Solutions

CD34⁺ cells are isolated using positive immunomagnetic cell separation procedures from umbilical cord blood. Human cord blood is collected aseptically at the time of delivery from healthy pregnant women under institutional review board (IRB) approved procedures.

Supplier: ProSci Inc.

Nierengewebelysat (7 Tage alt) wurde durch Homogenisierung in modifiziertem RIPA-Puffer (150 mM Natriumchlorid, 50 mM Tris-HCl, pH 7,4, 1 mM Ethylendiamintetraessigsäure, 1 mM Phenylmethylsulfonylfluorid, 1% Triton X-100, 1% Natriumdesoxycholsäure, 0,1% Natriumdodecylsulfat, 5 μg/ml Aprotinin, 5 μg/ml Leupeptin) hergestellt.

Supplier: Enzo Life Sciences

The PC-12 cell line was derived from a transplantable rat adrenal pheochromocytoma. These cells were maintained in Ham's F12K medium with L-glutamine (2 mM) adjusted to contain 1,5 g/L sodium bicarbonate (82,5%), horse serum (15%) and faetal bovine serum (2,5%).

Supplier: PROVITRO

HKER-d - Human keratinocytes from dermis - are obtained from human tissue, in particularly from the Dermis, initially isolated in their in vivo state. The cells are then transitioned into a primary cell culture and subsequently aliquoted and frozen for preservation. It's crucial to note that HKER cultures undergo careful methods during isolation, ensuring that they remain untransformed and unmutated, maintaining characteristics reflective of their in vivo state. These primary cultures have a limited in-vitro lifespan.

Supplier: Rockland Immunochemicals

Whole cell lysates are derived from cell lines tissues using highly refined extraction protocols to ensure quality, protein integrity and high reproducibility. Lysates are prepared in denaturing buffer without dissociating agents.

Supplier: Charles River Laboratories Cell Solutions

Human peripheral blood M1 macrophages are derived from purified monocytes. CD14⁺ monocytes were isolated from peripheral blood mononuclear cells (PBMCs) using positive immunomagnetic cell separation procedures and were cultured in the presence of GM-CSF for 10 days. The M1 phenotyping was confirmed by morphology and the expression of specific biomarkers.

Supplier: Novus Biologicals

BACH2 Overexpression Lysate (Adult Normal)

Supplier: ProSci Inc.

Lungengewebelysat (0 Tage alt) wurde durch Homogenisierung in modifiziertem RIPA-Puffer (150 mM Natriumchlorid, 50 mM Tris-HCl, pH 7,4, 1 mM Ethylendiamintetraessigsäure, 1 mM Phenylmethylsulfonylfluorid, 1% Triton X-100, 1% Natriumdesoxycholsäure, 0,1% Natriumdodecylsulfat, 5 μg/ml Aprotinin, 5 μg/ml Leupeptin) hergestellt.

Supplier: PROVITRO

Natural Killer cells (NK cells) constitute a lymphocytic subset of the immune system and play a crucial role in immunological surveillance and host defence. Their significance spans across various areas such as bone marrow transplantation, cancer, pregnancy, autoimmune diseases, viral infections, and increasingly in adaptive immunity.

Supplier: G-Biosciences

GenLysates™ are total protein tissue and cell lysates that are extracted from the tissue or cell of interest. To inhibit proteolysis, the GenLysates™ are prepared in a lysis buffer containing a complete protease inhibitor cocktail. Each lysate lot is tested by electrophoresis and Western blot analysis.

Supplier: G-Biosciences

GenLysates™ are total protein tissue and cell lysates that are extracted from the tissue or cell of interest. To inhibit proteolysis, the GenLysates™ are prepared in a lysis buffer containing a complete protease inhibitor cocktail. Each lysate lot is tested by electrophoresis and Western blot analysis.

Supplier: ProSci Inc.

Lebergewebelysat (0 Tage alt) wurde durch Homogenisierung in modifiziertem RIPA-Puffer (150 mM Natriumchlorid, 50 mM Tris-HCl, pH 7,4, 1 mM Ethylendiamintetraessigsäure, 1 mM Phenylmethylsulfonylfluorid, 1% Triton X-100, 1% Natriumdesoxycholsäure, 0,1% Natriumdodecylsulfat, 5 μg/ml Aprotinin, 5 μg/ml Leupeptin) hergestellt.

Supplier: ProSci Inc.

Lebergewebelysat (14 Tage alt) wurde durch Homogenisierung in modifiziertem RIPA-Puffer (150 mM Natriumchlorid, 50 mM Tris-HCl, pH 7,4, 1 mM Ethylendiamintetraessigsäure, 1 mM Phenylmethylsulfonylfluorid, 1% Triton X-100, 1% Natriumdesoxycholsäure, 0,1% Natriumdodecylsulfat, 5 μg/ml Aprotinin, 5 μg/ml Leupeptin) hergestellt.

Supplier: PROVITRO

HUASMC - Human umbilical artery smooth muscle cells - are obtained from human tissue, in particularly from the umbilical artery, initially isolated in their in vivo state. The cells are then transitioned into a primary cell culture and subsequently aliquoted and frozen for preservation. It's crucial to note that HUASMC cultures undergo careful methods during isolation, ensuring that they remain untransformed and unmutated, maintaining characteristics reflective of their in vivo state. These primary cultures have a limited in-vitro lifespan.