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Supplier: PROVITRO

CD34⁺ stem and progenitor cells from umbilical cord blood are crucial for haematopoiesis and modulation of the immune response. These unique cells contribute to the formation of various blood cells, including immune effectors such as natural killer cells and cytotoxic T-cells, shaping both the adaptive and functional aspects of the immune system.

Supplier: Charles River Laboratories Cell Solutions

CD19⁺ B cells are isolated from mononuclear cells by positive or negative selection using immunomagnetic cell separation procedures. Peripheral blood cells are collected from normal human volunteers by direct collection or by using the COBE® Spectra or Spectra Optia® Apheresis System where cell-rich leukapheresis product is extracted using continuous flow centrifugal technology directly into the collection bag.

Supplier: ProSci Inc.

Hautgewebelysat (14 Tage alt) wurde durch Homogenisierung in modifiziertem RIPA-Puffer (150 mM Natriumchlorid, 50 mM Tris-HCl, pH 7,4, 1 mM Ethylendiamintetraessigsäure, 1 mM Phenylmethylsulfonylfluorid, 1% Triton X-100, 1% Natriumdesoxycholsäure, 0,1% Natriumdodecylsulfat, 5 μg/ml Aprotinin, 5 μg/ml Leupeptin) hergestellt.

Supplier: Charles River Laboratories Cell Solutions

M1 and M 2 macrophages are derived from purified CD14⁺ monocytes. CD14⁺ monocytes were positively selected via immunomagnetic beads and were cultured in the presence of GM-CSF(M1) or M-CSF(M2) for 10 days. The phenotyping was confirmed by morphology and the expression of specific biomarkers.

Supplier: Charles River Laboratories Cell Solutions

Naive CD4⁺ T cells are isolated from mononuclear cells by positive selection using immunomagnetic cell separation procedures.

Supplier: ProSci Inc.

Lebergewebelysat (7 Tage alt) wurde durch Homogenisierung in modifiziertem RIPA-Puffer (150 mM Natriumchlorid, 50 mM Tris-HCl, pH 7,4, 1 mM Ethylendiamintetraessigsäure, 1 mM Phenylmethylsulfonylfluorid, 1% Triton X-100, 1% Natriumdesoxycholsäure, 0,1% Natriumdodecylsulfat, 5 μg/ml Aprotinin, 5 μg/ml Leupeptin) hergestellt.

Supplier: PROVITRO

HFIB-d - Human Fibroblast from dermis - are obtained from human tissue, in particularly from the dermis, initially isolated in their in vivo state. The cells are then transitioned into a primary cell culture and subsequently aliquoted and frozen for preservation. It's crucial to note that HFIB cultures undergo careful methods during isolation, ensuring that they remain untransformed and unmutated, maintaining characteristics reflective of their in vivo state. These primary cultures have a limited in-vitro lifespan.

Supplier: Charles River Laboratories Cell Solutions

Peripheral blood mononuclear cells (PBMC) are isolated from blood collected in FDA-registered collection centres, from IRB consented healthy human donors and following cGMP and cGTP collection guidelines.

Supplier: G-Biosciences

GenLysates™ are total protein tissue and cell lysates that are extracted from the tissue or cell of interest. To inhibit proteolysis, the GenLysates™ are prepared in a lysis buffer containing a complete protease inhibitor cocktail. Each lysate lot is tested by electrophoresis and Western blot analysis.

Supplier: Rockland Immunochemicals

Whole cell lysates are derived from cell lines tissues using highly refined extraction protocols to ensure quality, protein integrity and high reproducibility. Lysates are prepared in denaturing buffer without dissociating agents.

Supplier: Charles River Laboratories Cell Solutions

Human peripheral blood M1 macrophages are derived from purified monocytes. CD14⁺ monocytes were isolated from peripheral blood mononuclear cells (PBMCs) using positive immunomagnetic cell separation procedures and were cultured in the presence of GM-CSF for 10 days. The M1 phenotyping was confirmed by morphology and the expression of specific biomarkers.

Supplier: Novus Biologicals

BACH2 Overexpression Lysate (Adult Normal)

Supplier: ProSci Inc.

Lungengewebelysat (0 Tage alt) wurde durch Homogenisierung in modifiziertem RIPA-Puffer (150 mM Natriumchlorid, 50 mM Tris-HCl, pH 7,4, 1 mM Ethylendiamintetraessigsäure, 1 mM Phenylmethylsulfonylfluorid, 1% Triton X-100, 1% Natriumdesoxycholsäure, 0,1% Natriumdodecylsulfat, 5 μg/ml Aprotinin, 5 μg/ml Leupeptin) hergestellt.

Supplier: PROVITRO

Natural Killer cells (NK cells) constitute a lymphocytic subset of the immune system and play a crucial role in immunological surveillance and host defence. Their significance spans across various areas such as bone marrow transplantation, cancer, pregnancy, autoimmune diseases, viral infections, and increasingly in adaptive immunity.

Supplier: G-Biosciences

GenLysates™ are total protein tissue and cell lysates that are extracted from the tissue or cell of interest. To inhibit proteolysis, the GenLysates™ are prepared in a lysis buffer containing a complete protease inhibitor cocktail. Each lysate lot is tested by electrophoresis and Western blot analysis.

Supplier: G-Biosciences

GenLysates™ are total protein tissue and cell lysates that are extracted from the tissue or cell of interest. To inhibit proteolysis, the GenLysates™ are prepared in a lysis buffer containing a complete protease inhibitor cocktail. Each lysate lot is tested by electrophoresis and Western blot analysis.