Transformations-Reagenzien

Supplier: MP Biomedicals

The EZ-Yeast™ Transformation Kit is designed for high throughput transformation with efficiencies up to 10³ transformants per μg plasmid DNA. It is ideal for cases where a large number of transformations must be performed, but only a few transformants are needed. Perfect for a quick analysis of potentially positive two-hybrid colonies, it does not require fully competent cells prior to transformation.

Supplier: MP Biomedicals

Alkali-Cation™ Yeast Transformation Kit provides an efficient method for yeast transformation and has been successfully used to transform linear DNA into Pichia pastoris for protein expression studies.

Supplier: BTX

BTXpress Cytofusion® Medium C is an advanced electrofusion buffer designed especially for hybridoma production. The low conductivity buffer is specially formulated to minimise cell turbulence during cell alignment and heating during electrofusion, for robust cell fusion efficiency and high cell viability. BTXpress Cytofusion® Medium C is sterile filtered from the highest quality non animal, medical grade reagents.

Supplier: Thermo Fisher Scientific

For transformation of competent cells.

Supplier: BTX

BTXpress Cytoporation® Media T/T4 is an advanced electroporation buffer designed for use with the BTX AgilePulse MAX™ large volume electroporation system for in vitro delivery of DNA, RNA, oligonucleotides, and siRNA. The low conductivity buffer is specially formulated to minimise heating of solution during large volume electroporation for maximum transfection efficiency and high cell viability. BTXpress Cytoporation® Media T and T4 are sterile filtered from the highest quality non animal, medical grade reagents. Buffer can be directly diluted in complete growth media for post electroporation cell culture.

Supplier: BTX

Die BTXpress™ ist eine einzelne Pufferlösung, die entwickelt wurde, um Gene schnell und effizient in Säugerzellen einzubringen, die bisher durch chemische und andere nicht-virale Verfahren als 'schwer transfizierbar' betrachtet wurden. Die Transfektion mit dieser hochleistungsfähigen Elektroporationslösung ist beim Einbringen von DNA sowie siRNA in Säugerzellen gleichermaßen effizient.

Supplier: G-Biosciences

Fast Yeast Transformation™ is a rapid single step yeast transformation kit that takes less than 10 minutes to prepare competent yeast cells. The competent yeast cells can be used immediately or frozen for later use. This method is suitable for both circular and linear plasmid transformations.

Supplier: BTX

Flatpack chambers are primarily used for prokaryotic applications (bacterial or yeast transformations), however they are also often used for high efficiency stem cell transfection.

Supplier: Zymo Research

The Mix & Go kit convenient for the preparation of competent E. coli.