Nukleinsäure-Reagenzien

Supplier: AAT Bioquest

Sanger method is one of the most reliable and earliest DNA sequencing methods.

Supplier: AAT Bioquest

NED dye qPCR Calibration Solution supplied as a 10000X concentrate is compatible with a wide variety of qPCR instruments, including the ABI7500 Fast, QuantStudio™, and ViiA™ seven systems.

Supplier: Thermo Fisher Scientific

Der dNTP Mix enthält vorgefertigte wässrige Lösungen von dATP, dCTP, dGTP und dTTP, jeweils in einer Endkonzentration von 10 mM.

Supplier: Thermo Fisher Scientific

DreamTaq™ DNA Polymerase is an enhanced Taq DNA polymerase optimised for all standard PCR applications. It ensures higher sensitivity, longer PCR products and higher yields compared to conventional Taq DNA polymerase. DreamTaq™ DNA Polymerase uses the same reaction set-up and cycling conditions as conventional Taq DNA polymerase. Extensive optimisation of reaction conditions is not required. The enzyme is inhibited by dUTP but can incorporate modified nucleotides. Applications include routine PCR amplification of DNA fragments up to 6 kb from genomic DNA and up to 20 kb from viral DNA, RT-PCR, and generation of PCR products for TA cloning.

Supplier: MP Biomedicals

The GeneClean® III Kit is used to purify fragments of DNA from 200 bp to 20 kb from solutions and agarose gels.

Supplier: AAT Bioquest

Die farbstoffmodifizierten Desoxyuridin-5'-triphosphate (wie Aminoallyl-dUTP) können zur Herstellung farbstoffhaltiger DNA durch herkömmliche enzymatische Einbaumethoden wie umgekehrte Transkription, Nick-Translation, Random-Primed-Labeling oder PCR verwendet werden.

Supplier: AAT Bioquest

TAQuest™ FAST qPCR Master mix for TaqMan probes is a ready-to-use 2X solution optimised for qPCR and two-step RT-qPCR well suited to use for TaqMan gene expression assays.

Supplier: EDVOTEK

Jedes PCR-Kügelchen enthält Taq DNA-Polymerase, Taq DNA-Polymerasepuffer, dNTP-Mischung und MgCl₂. Packungsinhalt ausreichend für 25 Reaktionen.

Supplier: AAT Bioquest

Eine Echtzeit-Polymerase-Kettenreaktion (Echtzeit-PCR), auch bekannt als quantitative Polymerase-Kettenreaktion (qPCR), ist eine beliebte Labortechnik der Molekularbiologie, die auf der Polymerase-Kettenreaktion (PCR) basiert.

Supplier: MP Biomedicals

GeneClean® Turbo Kit is used for purification of DNA fragments of sizes 0,1 kb to 300 kb from TAE or TBE buffered agarose gels, PCR reactions and other enzymatic solutions. Purified DNA can be used in enzymatic reactions, conventional or automated sequencing, cloning and microarray analysis.

Supplier: AAT Bioquest

3'-O-Azidomethyl-dGTP ist eines der vier essentiellen dNTPs (A, C, G, T), die für die DNA-Sequenzierung durch Synthese (SBS) verwendet werden können.

Supplier: MP Biomedicals

SPINeasy® DNA Kit for Yeast is a high-performance genomic DNA (gDNA) extraction kit which is developed based on silica-membrane spin-column technology. This kit enables quick isolation of gDNA from yeast cells, typically in less than 30 minutes.

Supplier: Thermo Fisher Scientific

Taq DNA Polymerase is a highly thermostable DNA polymerase from the thermophilic bacterium Thermus aquaticus. The enzyme catalyses 5'→3' synthesis of DNA, has no detectable 3'→5' exonuclease (proofreading) activity and possesses low 5'→3' exonuclease activity. In addition, Taq DNA polymerase exhibits deoxynucleotidyl transferase activity, which frequently results in the addition of extra adenines at the 3'-end of PCR products. Recombinant Taq DNA Polymerase is ideal for standard PCR of templates 5 kb or shorter.

Supplier: FINNZYMES REAGENTS

Phusion® U multiplex PCR master mix is a ready-to-use, 2X end-point PCR master mix designed for simultaneous amplification of multiple targets in a single tube.

Supplier: OMEGA BIO-TEK

The E.Z.N.A.® DNA/RNA isolation kit is designed for the simultaneous isolation of both genomic DNA and total RNA from the same cells or tissues. The sample is first lysed and homogenised in a special denaturing buffer, spun to pellet RNA and undigested particles, and then the supernatant is applied to a HiBind® DNA spin column to bind DNA. The RNA pellet is dissolved and purified by a HiBind RNA spin column. Since there is no need to divide the sample into two parts for separate purification procedures, maximum yield of DNA and RNA can be purified from the entire sample.

Supplier: MP Biomedicals

The Fast DNA® SPIN Kit is used with the FastPrep®-24 or FastPrep® FP120 instrument to lyse and subsequently isolate DNA from up to 200 mg of almost any sample in less than 30 minutes.