Protein-Aufbereitungs

Supplier: Cytiva

Con A Sepharose is Concanavalin A coupled to Sepharose 4B by the cyanogen bromide method.

Supplier: Cytiva

Butyl Sepharose 4 Fast Flow is part of the Sepharose Fast Flow HIC platform, which has been an industrial standard for HIC chromatography during recent decades.

Supplier: Cytiva

DEAE Sephacel™ is a weak anion exchanger based on beaded cellulose. The ion exchange group is diethylaminoethyl, which remains charged and maintains consistently high capacity over the entire working range. This media is macroporous and has an exclusion limit of ~10000 kDa for globular proteins. Supplied pre-swollen in 20% ethanol.

Supplier: Cytiva

Epoxy-activated Sepharose™ 6B is a pre-activated medium for immobilisation of various ligands including sugars through coupling of hydroxy, amino or thiol groups on the ligand to Sepharose™ 6B via a 12-atom hydrophilic spacer arm. Medium is formed by reacting Sepharose™ 6B with 1,4-bis (2,3- epoxy-propoxy-) butane. It can be used to couple sugars and other carbohydrates via stable ether linkages to hydroxyl groups.

Supplier: Cytiva

MabSelect SuRe™ is composed of a rigid, high-flow agarose matrix and alkali-tolerant rProtein A ligand. The ligand has been engineered to provide greater stability than conventional protein A-based media in the alkaline conditions used in CIP protocols. This enhanced alkali stability improves process economy and product quality.

Supplier: Cytiva

Inline filter kit (10 ml/min systems)

Supplier: Cytiva

Schnelle und einfache Reinigung von GST-markierten Proteinen aus Bakterienlysaten und anderen Glutathion-S-Transferase- oder Glutathion-abhängigen Proteinen.

Supplier: Cytiva

Butyl Sepharose™ HP media is particularly well suited for intermediate purification and polishing steps providing high resolution due to the small particle size.

Supplier: G-Biosciences

The concentrator is a high molecular weight polymer which will not migrate across the dialysis membrane. The concentrator is placed against the dialysis membrane of the Tube-O-DIALYZER™, where it rapidly absorbs water from the sample and reduces the sample volume. The concentrator can be used with dialysis bags and other dialysis devices.

Supplier: Cytiva

ANX Sepharose™ 4 Fast Flow (high sub) is a weak anion exchanger, with a different selectivity compared with traditional ion exchangers.

Supplier: Cytiva

This media is suitable for affinity chromatography.

Supplier: Cytiva

DEAE Sephadex™ A-25 and DEAE Sephadex™ A-50 are weak anion exchangers. Sephadex™ ion exchangers are produced by introducing functional groups onto the cross-linked dextran matrix. These groups are attached to glucose units in the matrix by stable ether linkages.

Supplier: Cytiva

Lentil lectin is a metalloprotein, containing Ca₂₊ and Mn₂₊, which binds reversibly to polysaccharides and glycoconjugates containing glucose or mannose type sugars.

Supplier: Cytiva

Phenyl Sepharose 6 Fast Flow is a well established, standard aromatic hydrophobic interaction chromatography (HIC) resin for capture and intermediate purification requiring low to resin hydrophobicity

Supplier: Cytiva

Q Sepharose™ High Performance is composed of crosslinked agarose beads with a mean diameter of 34 µm, modified with quaternary (Q) strong anion exchange groups.

Supplier: Cytiva

Ni Sepharose™ High Performance affinity media for high resolution, high capacity purification of histidine-tagged proteins by immobilised metal ion affinity chromatography (IMAC).