Protein-Aufbereitungs
Proteinaufreinigungssysteme sind für die Isolierung eines oder mehrerer Proteine von einem komplexen Gemisch vorgesehen. Die Apparate können mit Zellen, Geweben oder ganzen Organismen verwendet werden und führen die Aufreinigung mit vordefinierten Startpunkten und bearbeitbaren Methodenvorlagen durch. Mit einem einzigen Klick bietet das kompakt geschnittene Gerät eine Laufsteuerung und -visualisierung in Echtzeit. Die sogar für den Betrieb in Kühlräumen geeigneten Produkte ermöglichen die Aufreinigung von affinitätsmarkierten Proteinen, Antikörpern, nicht markierten und nativen Proteinen sowie Probenaufreinigungen.
J.T.Baker® BAKERBOND® PROchievA™, Affinity Chromatography Resin, Recombinant Protein A
Supplier: Avantor
The J.T.Baker® BAKERBOND® PROchievA™ protein A resin offers superior dynamic binding capacity with excellent alkaline stability linked to Avantor's proprietary ligand.
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Lectin from Triticum vulgaris, immobilized on Agarose CL-4B, for affinity chromatography, Supelco®
Supplier: Merck
50% aqueous suspension in 1 M NaCl.
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Ion exchange chromatography media, Ion Exchanger I
Supplier: Merck
Ion Exchanger I is a strong acid cation exchangers widely used in separation of noble earths and amino acids, water treatment, analysis and food industry. The bond strength of equivalent ions increases with decreasing diameter of the hydrated ion.
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Protein-Niederschlagsplatten, J.T.Baker® BAKERBOND®
Supplier: Avantor
J.T.Baker® BAKERBOND® Protein-Niederschlagsplatten ermöglichen eine schnelle Proteinentfernung aus biologischen Proben.
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Gestützte Flüssigextraktionsplatten, J.T.Baker® BAKERBOND®
Supplier: Avantor
J.T.Baker® BAKERBOND® Platten für die unterstützte Flüssigextraktion (Supported Liquid Extraction, SLE) sind eine ideale und schnellere Alternative zu herkömmlichen Flüssig-Flüssig-Extraktionsmethoden und ermöglichen eine hervorragende Rückgewinnung von Analyten aus biologischen Matrices.
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Immunoprecipitation kit, Abcam
Supplier: Abcam
Abcam's Immunoprecipitation Kit (ab206996) can be used to perform immunoprecipitation (IP) and Co-IP for functional studies of immunoprecipitated proteins/complexes and SDS-PAGE or western blot analysis of immunoprecipitated proteins and complexes.
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Mouse TCS Antibody Purification Kit, Abcam
Supplier: Abcam
Efficient mouse antibody purification from tissue culture supernatants.
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Affinity purification Streptavidin Resin and buffers
Supplier: G-Biosciences
Streptavidin Resin is designed for the single step small and large scale affinity purification of proteins and antibodies with a biotin tag. Biotin, a 244 Da vitamin (Vitamin H) molecule, exhibits an extraordinary binding affinity for avidin (Ka=10¹⁵ M⁻¹) and streptavidin (Ka=10¹⁵ M⁻¹). Biotin and (strept)avidin interaction is rapid and once the bond is established it can survive up to 3M guanidinehydrochloride and extremes of pH. Biotin-avidin bonds can only be reversed by denaturing the avidin protein molecule with 8M guanidine-hydrochloride at pH 1,5 or by autoclaving. The biotinylated molecules are efficiently probed with avidin or streptavidin conjugated to reporter molecules, such as peroxidases or phosphatases.
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BSA Removal Kit (Gold/Europium labelling), Abcam
Supplier: Abcam
Our BSA Removal Kit - Nanoparticles (ab204912) is a simple one-step, 10 minute method that effectively separates BSA from the antibody.
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GTP-Agarose, Abcam
Supplier: Abcam
GTP-Agarose (ab270533) comprises GTP attached to agarose beads.
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HRP Conjugate Stabilizer - LifeXtend, Abcam
Supplier: Abcam
HRP Conjugate Stabilizer - LifeXtend (ab270548) is a proprietary multi-component reagent system that protects antibody–HRP conjugates from instability, microbrial attack, and denaturation, thus ensuring the best possible performance in experiments performed at room temperature.
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Medien für die hydrophobe Interaktionschromatographie, J.T. Baker®, BAKERBOND®
Supplier: Avantor
Medien auf Basis von Siliziumdioxid, die für die Proteinreinigung auf der Grundlage unterschiedlicher salzinduzierter Hydrophobizität verwendet werden. Der Mischmodus bietet eine einzigartige Selektivität durch seine hydrophobe Wechselwirkung und die Ionenaustauschfunktion.
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Protein G
Supplier: Rockland Immunochemicals
Protein G is a surface protein of two groups of streptococcal bacteria that has ability to bind immunoglobulins and also to albumins. Protein G are prepared from chromatographically pure protein G.
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Llama affinity purification resin
Supplier: ImmunoReagents
ImmunoReagents Llama IgG Affinity Prep resins are prepared using highly purified species specific immunoglobulins. This resin is suitable for removal of proteins recognising Llama IgG from liquid solutions, including traditional Immunoabsorptions and Immunoprecipitation protocols.
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Serum Antibody Purification Kit (Protein G), Abcam
Supplier: Abcam
Protein G has a high affinity for the Fc regions of IgG molecules from a variety of species.
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Human affinity purification resin
Supplier: ImmunoReagents
ImmunoReagents Human IgG Affinity Prep resins are prepared using highly purified species specific immunoglobulins. This resin is suitable for removal of proteins recognising Human IgG from liquid solutions, including traditional Immunoabsorptions and Immunoprecipitation protocols.
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