Proteintests

Supplier: AAT Bioquest

Optimised to use with Nanodrop. Amplite™ colorimetric biotin quantitation kit is designed to quantify the number of biotins attached to a protein or other macromolecule with a nanodrop spectrometer.

Supplier: Thermo Fisher Scientific

The Pierce™ Coomassie Plus Protein assay is a quick and ready-to-use, reducing agent-compatible, improved Bradford assay to quickly measure (A595 nm) total protein concentration compared to a protein standard.

Supplier: Thermo Fisher Scientific

Biotinylated horse radish peroxidase is used as control or signal amplification in IHC to amplify the signal of biotinylated primary antibodies using the avidin-biotin complex staining method.

Supplier: AAT Bioquest

Designed for consistent and dependable results. Amplite® Fluorimetric Biotin Quantitation Kit uses Biotinylite™ Green, a fluorogenic biotin sensor.

Supplier: Thermo Fisher Scientific

Thermo Scientific™ Pierce™ Rapid Gold BCA Protein Assay Kit is a two-component, high precision, detergent-compatible assay optimised to measure (at 480 nm) total protein concentration compared to a protein standard curve of known concentrations. The Pierce™ Rapid Gold BCA Protein Assay uses the same copper-chelating technology as the well-known traditional Pierce™ BCA Protein Assay and provides comparable accuracy but with a 5 minute, room temperature incubation and measured at 480 nm. This improvement eliminates the need to wait or expose the samples to elevated temperatures for a fast time to results.

Supplier: Thermo Fisher Scientific

Pierce™ Phosphoprotein Phosphate Estimation Assay Kit enables classification and identification of proteins as phosphorylated (serine and threonine), as well as semi-quantitative assessment of the phosphorylation level.

Supplier: G-Biosciences

A simple and reliable method for estimation of protein contamination in latex. Detects as low as 25 to 40 μg protein/gm latex (or 25 to 50 ppm). Protein solutions applied to the test strips form compact and symmetrical spots, their diameter being directly proportional to protein concentration. Protn-Latex™ latex protein extraction buffer extracts protein from the latex sample, which is then applied to the test strips and rapidly developed. By measuring the Ø of protein spots with the Protn-Latex™ card, the range of protein concentration in the sample can be determined.

Supplier: Boster Bio

The Micro BCA Protein Assay Kit is a ready to use, detergent-compatible, Western blot related, total protein analysis reagent used for the quick determination of total protein concentration of dilute protein solutions by measuring absorbance at 562 nm and comparing to a protein standard absorbance vs. concentration curve.

Supplier: Merck Millipore (Calbiochem‎)

Non-Interfering Protein Assay™ Kit is an easy-to-use protein assay that overcomes interference of agents found in protein solutions including detergents, chelating agents, reducing agents, amines, sugars, urea, and others. The Universal Protein Precipitating Agent (UPPA™ Reagent) is used to precipitate and immobilize the protein in the tube while the interfering reagents are removed.

Supplier: Thermo Fisher Scientific

Thermo Scientific™ Pierce™ Quantitative Colorimetric Peptide Assay is an easy-to-use colorimetric microplate assay designed specifically to improve the sensitivity and reproducibility of quantitation of peptide mixtures.

Supplier: Abcam

Simple assay to detect total glutathione.

Supplier: Thermo Fisher Scientific

Pierce™ Streptavidin Poly-HRP conjugate is biotin-binding protein conjugated with polymers of horseradish peroxidase, enabling signal amplification and detection of biotinylated antibodies for IHC and other methods.

Supplier: Abnova

Biotin Labelling Kit is used for simple and rapid preparation of biotin-labeled antibodies and proteins.

Supplier: Cytiva

2-D Quant Kit is designed for the accurate determination of protein concentration in samples prepared for electrophoresis techniques such as 2-D electrophoresis, SDS-PAGE, or IEF.

Supplier: MP Biomedicals

Histones are soluble in water (10 mg/ml) or 0,5 N HCl (10 mg/ml), yielding a clear to hazy solution depending on the fraction. They are soluble in 6 M urea (4 to 10 mg/ml), but this will denature the histones.

Supplier: Uptima

Die BCA-Methode ist ein sehr empfindlicher Proteinnachweis. Peptidbindungen und die vier Aminosäuren Cystein, Cystin, Tryptophan und Tyrosin sollen für die Bestimmung bedeutsam sein. Die Bildung von Cu(I)-Ionen durch Proteine unter alkalischen Bedingungen entspricht dabei der Biuret Bestimmungsmethode. Ein Cu(I)-Ion wird dann durch zwei Moleküle BCA zu einem roten Farbkomplex gebunden. Dieser Komplex kann sehr empfindlich nachgewiesen werden.