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Proteintests

Accurately quantifying total protein concentration is a key to most experiments and is often necessary for isolation, separation, and analysis of proteins by biochemical methods. When it is necessary to determine the total protein concentration in a sample, one of the first factors to consider is the selection of a protein assay method. These choices are typically based upon the compatibility of the method with the samples to be assayed. Each method has its own advantages and disadvantages.

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BCA protein assay kit (BCA-PAK)

BCA protein assay kit (BCA-PAK)

Supplier: OZ BIOSCIENCES

BCA Protein Assay kit (BCA-PAK) is useful for colorimetric detection and quantification of total protein content, even in the presence of detergents.

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Solid phase biotinylation kits, EZ-Link™, Pierce™

Solid phase biotinylation kits, EZ-Link™, Pierce™

Supplier: Thermo Fisher Scientific

This antibody labeling system uses nickel chelated agarose to temporarily immobilise antibody molecules via their histidine-rich Fc regions. Once held in place on the gel, the antibody can be biotinylated at either sulfhydryl groups (after mild reduction or disulfide bonds) or primary amines. Excess labeling reagent and by-products are then washed away before recovering the labeled and purified antibody from the gel using a mild imidazole solution. No gel filtration or dialysis are needed.

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Pierce™ Polyhis-Protein Interaction Pull Down Kit

Pierce™ Polyhis-Protein Interaction Pull Down Kit

Supplier: Thermo Fisher Scientific

The Pierce™ his protein interaction pull-down kit contains the necessary components to capture and purify proteins that interact with his-tagged fusion proteins.

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Pierce™ Glycoprotein-Kohlenhydrat-Schätzkits

Pierce™ Glycoprotein-Kohlenhydrat-Schätzkits

Supplier: Thermo Fisher Scientific

Mit dem Pierce™ Glycoprotein Carbohydrate Estimation Kit kann der Grad der Proteinglykosylierung als Prozentsatz der gesamten gereinigten Proteinmasse gemessen werden.

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Modified lowry protein assay kit, Pierce™

Modified lowry protein assay kit, Pierce™

Supplier: Thermo Fisher Scientific

The Modified Lowry Protein Assay Kit is the preferred colorimetric assay for quantifying total protein concentration.

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Pierce™ BCA™ Protein assay kits and reagents

Pierce™ BCA™ Protein assay kits and reagents

Supplier: Thermo Fisher Scientific

Pierce BCA™ (bicinchoninic acid) reagents provide accurate determination of protein concentration with most sample types encountered in protein research. The Pierce BCA™ Protein Assay can be used to assess yields in whole cell lysates and affinity-column fractions, as well as to monitor protein contamination in industrial applications. Compared to most dye-binding methods, the BCA™ Assay is affected much less by protein compositional differences, providing greater protein-to-protein uniformity.

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Portelite™ Rapid Fluorimetric Biotin Quantitation Kit

Supplier: AAT BIOQUEST

Optimised to use with CytoCite™ and Qubit™ fluorometers. Portelite™ rapid fluorimetric biotin quantitation kit is specifically optimised to quantify biotin and biotin conjugates with a portal fluorometer such as CytoCite™ and Qubit™ fluorometer.

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Portelite™ Fluorimetric Protein Quantitation Kit

Supplier: AAT BIOQUEST

Optimised for CytoCite™ and Qubit™ fluorometers. The Portelite™ fluorimetric protein quantitation kit is significantly more sensitive than existing colorimetric protein measurements, e.g., Bradford and Bicinchoninic acid (BCA) assays.

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Quantitatives fluorometrisches Peptid-Assay, Pierce™

Supplier: Thermo Fisher Scientific

Das Thermo Scientific™ Pierce™ quantitative Fluoreszenz-Peptidassay ist ein empfindliches Misch-und-Mess-Fluoreszenz-Mikrotiterplattenassay für die quantitative Messung von Peptiden und Peptidmischungen.

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Coomassie (Bradford) protein assay kit, Pierce™

Coomassie (Bradford) protein assay kit, Pierce™

Supplier: Thermo Fisher Scientific

The Pierce™ Coomassie (Bradford) Protein Assay Kit is a ready-to-use, stable formulation of the traditional Bradford assay reagent to measure (A595 nm) total protein concentration compared to a protein standard.

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Portelite™ Fluorimetric Protein Quantitation Kit, Optimized for CytoCite™ and Qubit™ Fluorometers

Portelite™ Fluorimetric Protein Quantitation Kit, Optimized for CytoCite™ and Qubit™ Fluorometers

Supplier: AAT BIOQUEST

Protein quantification is an essential task in protein purification, electrophoresis, cell biology, molecular biology and other research applications.

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Pierce™ BCA Protein Assay Kits - Reducing Agent Compatible

Pierce™ BCA Protein Assay Kits - Reducing Agent Compatible

Supplier: Thermo Fisher Scientific

This BCA Protein Assay Kit - Reducing Agent Compatible enables the measurement of protein concentration in samples that contain thiol-reductants dithiothreitol (DTT) and 2-mercaptoethanol (BME).

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Tetracysteine REASH Reagent *2 mM DMSO Solution*

Tetracysteine REASH Reagent *2 mM DMSO Solution*

Supplier: AAT BIOQUEST

REASH is a resorufin derivative, modified to contain two arsenic atoms at a set distance from each other.

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Pierce™ Protease Assay Kits, Colorimetric and Fluorometric

Pierce™ Protease Assay Kits, Colorimetric and Fluorometric

Supplier: Thermo Fisher Scientific

Pierce™ Protease Assay Kits are an ideal choice for performing routine assays necessary during the isolation of proteases, or for identifying the presence of contaminating proteases in protein samples. These protease assays are also ideal for studying pH or temperature vs. activity profiles of purified proteases.

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Biotinylated-protein interaction pull down kit, Pierce™

Biotinylated-protein interaction pull down kit, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ biotinylated protein interaction pull-down kit contains the necessary components to capture and purify interactors of a biotin-labeled protein or ligand.

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Pierce™ Coomassie Plus (Bradford) Protein Assay Kit

Pierce™ Coomassie Plus (Bradford) Protein Assay Kit

Supplier: Thermo Fisher Scientific

The Pierce™ Coomassie Plus Protein assay is a quick and ready-to-use, reducing agent-compatible, improved Bradford assay to quickly measure (A595 nm) total protein concentration compared to a protein standard.

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Amplite® Fluorimetric Biotin Quantitation Kit

Supplier: AAT BIOQUEST

Designed for consistent and dependable results. Amplite® Fluorimetric Biotin Quantitation Kit uses Biotinylite™ Green, a fluorogenic biotin sensor.

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Horseradish Peroxidase, Biotinylated, Pierce™

Horseradish Peroxidase, Biotinylated, Pierce™

Supplier: Thermo Fisher Scientific

Biotinylated horse radish peroxidase is used as control or signal amplification in IHC to amplify the signal of biotinylated primary antibodies using the avidin-biotin complex staining method.

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Phosphoprotein phosphate estimation assay Kit, Pierce™

Phosphoprotein phosphate estimation assay Kit, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ Phosphoprotein Phosphate Estimation Assay Kit enables classification and identification of proteins as phosphorylated (serine and threonine), as well as semi-quantitative assessment of the phosphorylation level.

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Pierce™ Micro BCA™ Protein Assay Kit and Reagents

Pierce™ Micro BCA™ Protein Assay Kit and Reagents

Supplier: Thermo Fisher Scientific

Micro BCA™ protein assay kit is a specialised version of Pierce™ BCA protein assay for determining the protein concentration of dilute samples.

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Marfey's reagent (FDAA or 1-fluoro-2-4-dinitrophenyl-5-L-alanine amide), Pierce™

Marfey's reagent (FDAA or 1-fluoro-2-4-dinitrophenyl-5-L-alanine amide), Pierce™

Supplier: Thermo Fisher Scientific

Marfey's Reagent (FDAA or 1-fluoro-2-4-dinitrophenyl-5-L-alanine amide) reacts with primary amines to enable the quick and easy separation and quantitation of optical isomers of amino acids by reverse-phase chromatography.

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Compat-Able™ Protein assay preparation reagent set

Compat-Able™ Protein assay preparation reagent set

Supplier: Thermo Fisher Scientific

The Compat-Able™ Protein assay preparation reagent set eliminates interfering substances from protein assay samples to make them compatible with BCA and Bradford assays.

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Coomassie Plus™ protein assay kit, Compat-Able™

Supplier: Thermo Fisher Scientific

The Thermo Scientific™ Compat-Able™ Coomassie Plus Protein Assay Kit eliminates interfering substances from protein assay samples to make them compatible with BCA and Bradford assays.

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Firefly luciferase flash assay kit, Pierce™

Firefly luciferase flash assay kit, Pierce™

Supplier: Thermo Fisher Scientific

The Pierce™ Firefly Luciferase Flash Assay Kit provides reagents to measure red firefly luciferase activity in whole cell producing a very strong flash signal which decays rapidly.

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Antibody labelling kits, HisTag® Antibody HRP Conjugate kit

Supplier: Merck Millipore (Novagen)

The HisTag® Antibody HRP Conjugate kit contains a horseradish peroxidase-conjugated HisTag® Monoclonal Antibody that is useful as a single detection reagent, thus eliminating cross-reactivity associated with secondary antibody reagents. It recognises five consecutive histidine residues regardless of the surrounding amino acid context. The purified antibody conjugate produces a strong signal at a 1:1000 to 1:2000 dilution on Western blots and dot blots. The HisTag® Antibody HRP Conjugate also detects the recombinant marker bands in the Novagen Perfect Protein™ Western Markers for convenient visualisation of accurate internal standards on Western blots.

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Octet® Anti-penta-HIS (HIS1K) biosensors

Supplier: Sartorius

For label-free quantitation and kinetic analysis of his-tagged proteins.

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Surface protein (allergen) test swab ALLTEC60, Clean-Trace™

Surface protein (allergen) test swab ALLTEC60, Clean-Trace™

Supplier: 3M Food Safety

3M™ Clean-Trace™ Surface Protein (Allergen) Test Swab ALLTEC60 is a rapid, simple, and reliable solution to monitor the presence of allergens on surfaces to effectively manage cleaning.

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Surface protein plus test swabs, PRO100, Clean-Trace™

Surface protein plus test swabs, PRO100, Clean-Trace™

Supplier: 3M Food Safety

3M™ Clean-Trace™ Surface Protein Plus Test Swab PRO100 is a simple, quick, and reliable method of checking for the removal of protein residues after cleaning and quickly identifies contamination.

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Tetracysteine FLASH Reagent *2 mM DMSO Solution*

Tetracysteine FLASH Reagent *2 mM DMSO Solution*

Supplier: AAT BIOQUEST

FLASH is a fluorescein derivative, modified to contain two arsenic atoms at a set distance from each other.

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ReadiUse™ TCA Deproteinization Sample Preparation Kit

ReadiUse™ TCA Deproteinization Sample Preparation Kit

Supplier: AAT BIOQUEST

The analysis of amino acids, ions, metabolites and other small molecules is frequently hindered by the interference of lipids, protein and enzymes present in biofluids, cell and tissue lysates.

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