Either measuring substrate consumption or overall production, the enzyme assays are used to monitor enzymatic activity. Provided with the required supplies and reagents for any preferred method, enzyme kinetics and inhibition studies can be successfully completed. While continuous assay sampling methods give activity readings throughout development, discontinuous techniques calculate after the reaction has been stopped. The standardized enzyme assays will provide accurate identification and measurement of the protein molecules present in samples.
MMP inhibitor profiling kit
Supplier: ENZO LIFE SCIENCES
The MMP inhibitor profiling kit, fluorometric RED is a complete assay system designed to examine the specificity of inhibitors against a panel of ten matrix metalloproteinase enzymes, using a quenched fluorogenic substrate OMNIMMP® RED.
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Soluble Epoxide Hydrolase Inhibitor Screening Kit (Fluorometric)
Supplier: Abcam
Simplest high-throughput assay to screen thioredoxin 1 inhibitors.
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Glutaminase (GLS1) Inhibitor Screening Kit (Fluorometric)
Supplier: Abcam
Sensitive, reproducible assay for initial screening of potential glutaminase inhibitors.
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Neutrophil Elastase Activity Assay Kit
Supplier: Cayman Chemical
A convenient all-in-one tool to measure neutrophil elastase activity.
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Arginase I (ARG1) Inhibitor Screening Kit (Colorimetric)
Supplier: Abcam
The most sensitive kit to evaluate potential human Arginase I inhibitors.
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Matrix Metalloproteinase-10 (MMP-10) Fluorometrisches Wirkstoffentdeckungs-Kit
Supplier: ENZO LIFE SCIENCES
Ein QuantiZyme™ Assay System. Das MMP-10 Fluorometrisches (auch bekannt als fluorimetrisch) Wirkstoffentdeckungs-Kit ist ein vollständiges Assay-System, das zum Screening von Inhibitoren der Matrix-Metalloproteinase-10 (MMP-10, Stromelysin-2) unter Verwendung eines gequenchten, fluorogenen Peptids entwickelt wurde: Fluorogenes OmniMMP™-Substrat Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH. Die Mca-Fluoreszenz wird durch die Dpa-Gruppe gequencht, bis die Spaltung durch MMPs an der Gly-Leu-Bindung die zwei Einheiten trennt.
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Sclerostin-LRP interaction screening system, LEADING LIGHT®
Supplier: ENZO LIFE SCIENCES
Novel Cell-free Sclerostin/LRP Binding Assay Ideal for Primary Inhibitor Screens.
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Matrix Metalloproteinase-2 (MMP-2) Kolorimetrisches Wirkstoffentdeckungs-Kit
Supplier: ENZO LIFE SCIENCES
Ein QuantiZyme™ Assay System. Das MMP-2 Kolorimetrisches Wirkstoffentdeckungs-Kit ist ein vollständiges Assaysystem, das zum Screening von Inhibitoren der Matrix-Metalloproteinase-2 (MMP-2, Stromelysin-1) unter Verwendung eines Thiopeptids als chromogenes Substrat entwickelt wurde. Die Assays werden in einem praktischen Mikroplattenformat mit 96 Wells durchgeführt. Das Kit ist nützlich, um Inhibitoren von MMP-2, einem potenziellen therapeutischen Ziel, zu screenen.
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Amplite® Fluorimetric α-Ketoglutarate Quantitation Kit
Supplier: AAT BIOQUEST
Alpha-ketoglutarate (α-ketoglutarate) is a key molecule in the Krebs cycle determining the overall rate of the citric acid cycle of the organism.
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Amplite® Fluorimetric Pyruvate Assay Kit
Supplier: AAT BIOQUEST
Pyruvate is an important chemical compound in intracellular metabolic pathways.
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Amplite® Rapid Colorimetric PE-Maleimide Quantitation Kit
Supplier: AAT BIOQUEST
Designed for consistent and dependable results. The Amplite® rapid colorimetric PE-maleimide quantitation kit facilitates the quantification of maleimide groups on maleimide-activated PE proteins using a proprietary sensor, Maleimide 680™, which has an absorption peak at ~680 nm.
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Pierce™ Gaussia Luciferase Flash Assay Kit
Supplier: Thermo Fisher Scientific
The Thermo Scientific Pierce Gaussia Luciferase Flash Assay Kit provides researchers with the most sensitive assay for transcriptional activity of regulatory elements in mammalian cell culture media and whole cell lysate.
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Serine hydrolase probes, ActivX™ FP
Supplier: Thermo Fisher Scientific
The ActivX® FP serine hydrolase probes enable selective labeling and enrichment of active serine hydrolases. Fluorophosphonate (FP) probes specifically label serines of enzymatically active serine hydrolases. FP probes are also effective for screening small molecule inhibitors against enzymes derived from cell lysates, subcellular fractions, tissues and recombinant proteins. Depending on the active-site probe tag group and the enzyme class to be profiled, active site-labeled enzymes are detected and quantified by Western blot, fluorescent gel imaging or mass spectrometry.
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Amplite® Red Peroxidase Substrate
Supplier: AAT BIOQUEST
Amplite® Red is a sensitive fluorogenic peroxidase substrate that generates a highly red fluorescent product that has maximum absorption of 571 nm and maximum emission of 585 nm.
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Amplite® Fluorimetric Protein Quantitation Kit *Orange Fluorescence*
Supplier: AAT BIOQUEST
Protein quantification is an essential task in protein purification, electrophoresis, cell biology, molecular biology and other research applications.
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Amplite® Fluorimetric Xanthine Oxidase Assay Kit *Red Fluorescence*
Supplier: AAT BIOQUEST
Xanthine oxidase (XO) is an enzyme that catalyses the oxidation of hypoxanthine to xanthine and can further catalyze the oxidation of xanthine to uric acid.
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Amplite® Fluorimetric Myeloperoxidase Assay Kit *Red Fluorescence*
Supplier: AAT BIOQUEST
Myeloperoxidase (MPO), most abundantly present in neutrophils and monocytes, is a green hemoprotein having peroxidase activity.
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Peroxide assay kit, PEROXsay™
Supplier: G-Biosciences
PEROXsay™ is a colorimetric quantitative peroxide assay that measures the oxidation of ferrous (Fe²⁺+) ions to ferric (Fe³⁺) ions. Peroxides react with a sugar alcohol converting it to a peroxyl radical that subsequently starts the oxidation of ferrous ions to ferric ions. The acidic pH of the assay allows the ferric (Fe³⁺) ion to complex with the colorimetric reagent, resulting in a change in absorbance that is proportional to the peroxide concentration.
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MMP-3 Green™ Substrate
Supplier: AAT BIOQUEST
The matrix metalloproteinases (MMPs) constitute a family of zinc-dependent endopeptidases that function within the extracellular matrix.
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Amplite® Ethanol Quantitation Kit
Supplier: AAT BIOQUEST
The ability to rapidly perform quantitative measurements of ethanol is highly desirable in life science research, clinical evaluations, and food and drug industries.
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ONPG assay kit
Supplier: OZ BIOSCIENCES
ONPG assay kit is ideal to quantify high expression level of beta-Gal.
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Amplite® Fluorimetric Tyrosinase Assay Kit
Supplier: AAT BIOQUEST
Tyrosinase is an enzyme expressed across a vast range of species from bacteria and fungi to animals.
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Amplite® Fluorimetric Monoamine Oxidase Assay Kit *Red Fluorescence*
Supplier: AAT BIOQUEST
Monoamine oxidases (MAO) are a family of flavin-containing amine oxidoreductases that catalyse the oxidation of monoamines.
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Amplite® Fluorimetric NADH Assay Kit *Red Fluorescence*
Supplier: AAT BIOQUEST
Nicotinamide adenine dinucleotide (NAD+) and nicotinamide adenine dinucleotide phosphate (NADP+) are two important cofactors found in cells.
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Amplite® Colorimetric Peroxidase (HRP) Assay Kit *Blue Color*
Supplier: AAT BIOQUEST
Peroxidase is a small molecule (MW ~40 KD) that can usually be conjugated to an antibody in a 4:1 ratio.
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Amplite® Fluorimetric Proteasome 20S Activity Assay Kit *Green Fluorescence*
Supplier: AAT BIOQUEST
Our Amplite® Fluorometric 20S Proteasome Assay Kit is a homogeneous fluorescent assay that measures the chymotrypsin-like protease activity associated with the proteasome complex either in cultured cells or cell lysates.
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Amplite® Colorimetric L-Lactate Dehydrogenase (LDH) Assay Kit
Supplier: AAT BIOQUEST
Lactate dehydrogenase (LDH) is an oxidoreductase enzyme that catalyses the interconversion of pyruvate and lactate.
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Amplite® Fluorimetric Glucose-6-Phosphate Dehydrogenase (G6PD) Assay Kit
Supplier: AAT BIOQUEST
Glucose-6-phosphate dehydrogenase (G6PD) catalyses the conversion of glucose-6-phosphate to 6-phosphoglucono-δ-lactone, the first and rate-limiting step in the pentose phosphate pathway.
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Amplite® Fluorimetric Calcium Quantitation Kit *Red Fluorescence*
Supplier: AAT BIOQUEST
Calcium is essential for all living organisms, particularly in cell physiology, where movement of the calcium ion Ca²⁺ into and out of the cytoplasm functions as a signal for many cellular processes.
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Amplite® Colorimetric Asparaginase Activity Assay Kit
Supplier: AAT BIOQUEST
Designed for consistent and dependable results. The Amplite® colorimetric asparaginase activity assay kit provides a simple and straightforward procedure for measuring asparaginase activity in a variety of biological samples.