Enzymtests
Enzymassays dienen der Überwachung der Enzymaktivität, sei es zur Messung des Substratverbrauchs oder der Gesamtproduktion. Mit den erforderlichen Materialien und Reagenzien für die jeweilige Methode lassen sich Enzymkinetik- und Hemmungsstudien erfolgreich durchführen. Kontinuierliche Probenahmeverfahren liefern Aktivitätswerte während des gesamten Reaktionsprozesses, während diskontinuierliche Techniken die Messung nach Reaktionsstopp ermöglichen. Standardisierte Enzymassays gewährleisten die präzise Identifizierung und Quantifizierung der in den Proben vorhandenen Proteinmoleküle.
Yeast β-galactosidase assay kit, Pierce™
Supplier: Thermo Fisher Scientific
Yeast β-Galactosidase Assay Kit is specially formulated for yeast cells and ideal for identifying protein interactions in vivo using two-hybrid systems.
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Amplite® Fluorimetric NADPH Assay Kit *Red Fluorescence*
Supplier: AAT Bioquest
Nicotinamide adenine dinucleotide (NAD+) and nicotinamide adenine dinucleotide phosphate (NADP+) are two important cofactors found in cells.
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Amplite® Colorimetric Butyrylcholinesterase Activity Assay Kit
Supplier: AAT Bioquest
Butyrylcholinesterase (EC 3.1.1.8; BChE), also known as pseudocholinesterase or plasma cholinesterase, is mainly synthesised in liver and present in blood.
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Amplite® Fluorimetric Sphingomyelin Assay Kit *Red Fluorescence*
Supplier: AAT Bioquest
Sphingomyelin (SM) is largely found in the exoplasmic leaflet of the cell membrane, primarily in nervous tissue.
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Amplite® Fluorimetric Goat Anti-Mouse IgG-HRP Conjugate ELISA Assay Kit *Red Fluorescence*
Supplier: AAT Bioquest
Horseradish Peroxidase (HRP) is a small molecule (MW ~40 Kd) that is widely used in a variety of biological detections.
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Amplite® Colorimetric Oxaloacetate Assay Kit *Red Color*
Supplier: AAT Bioquest
Oxaloacetate is an important part of citric acid cycle, where it reacts with Acetyl-CoA to form citrate.
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Amplite® Rapid Colorimetric Protein Aldehyde Content Quantitation Kit
Supplier: AAT Bioquest
Site-selective modification of proteins is of major interest in chemical biology.
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β-Galactosidase Assay Kit and Reagents
Supplier: Thermo Fisher Scientific
The Mammalian β-Galactosidase Assay Reagent Kit is a simple and versatile assay to measure β-galactosidase activity in cultured mammalian cells. Cells grown on a plate may be lysed and tested for β-galactosidase activity by adding a single all-in-one lysis-and-assay solution. Alternatively, M-PER™ Mammalian Protein Extraction Reagent may be used to prepare lysate from washed adherent or pelleted cells, and then a portion of the lysate used to assay for β-galactosidase activity. With M-PER Reagent, there is no need to scrape cells or to perform freeze-thaw cycles. The remaining lysates prepared with M-PER™ Reagent may be used for applications such as reporter assays (luciferase, β-galactosidase and chloramphenical acetyltransferase), protein kinase assays (PKA, PKC, and tyrosine kinase), immunoassays (Western blot, ELISA, RIA) and protein purification.
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Amplite® Fluorimetric Total Thiol Quantitation Assay Kit *Green Fluorescence*
Supplier: AAT Bioquest
The detection and measurement of free thiol (such as free cysteine, glutathione and cysteine residues in proteins) is one of the essential tasks for investigating biological processes and events in many biological systems.
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Amplite® Fluorimetric Total NAD and NADH Assay Kit *Red Fluorescence*
Supplier: AAT Bioquest
Nicotinamide adenine dinucleotide (NAD+) and nicotinamide adenine dinucleotide phosphate (NADP+) are two important cofactors found in cells.
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Amplite® Fluorimetrisches Beta-Galactosidase-Assay-Kit *Grüne Fluoreszenz*
Supplier: AAT Bioquest
E. coli Beta-Galactosidase ist ein 464 Kd-Tetramer.
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Amplite® Colorimetric Zinc Ion Quantitation Kit
Supplier: AAT Bioquest
Zinc is an essential trace mineral element that plays an important role in a number of biological processes.
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Amplite® Fluorimetrisches Wasserstoffperoxid-Assay-Kit *Rote Fluoreszenz*
Supplier: AAT Bioquest
Wasserstoffperoxid (H₂O₂) ist ein reaktives Nebenprodukt des Sauerstoffstoffwechsels, das als Schlüsselregulator für eine Reihe von Zuständen im Zusammenhang mit oxidativem Stress dient.
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Amplite® Fluorimetric Neutrophil Elastase Activity Assay Kit
Supplier: AAT Bioquest
Designed for consistent and dependable results. The Amplite® fluorimetric neutrophil elastase activity assay kit provides a robust and accurate method for quantifying neutrophil elastase (NE) activity.
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Amplite® Colorimetric Bradford Protein Quantitation Assay Kit
Supplier: AAT Bioquest
The traditional Bradford protein assay is widely used for quantifying protein concentrations.
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Amplite® Colorimetric Glucose and Sucrose Assay Kit
Supplier: AAT Bioquest
Designed for consistent and dependable results. The Amplite® Colorimetric Glucose and Sucrose Assay Kit offers a convenient method for measuring glucose and sucrose levels in various biological samples, such as serum, plasma, body fluids, food, tissue extracts, and growth medium.
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