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Cellular assays have always been a powerful tool in the research lab. They are incredibly versatile, and can be designed to measure virtually any cellular or biochemical function. Sensitive detection of fluorescent probes aid in evaluating cell signalling and death pathways, which is useful for the rapid quantification of cell viability. Cellular assays can be used to measure the effects of different treatments on cell viability and to evaluate toxic effects mediated through a variety of mechanisms.
NUCLEAR-ID® green chromatin condensation detection kit
Supplier: ENZO LIFE SCIENCES
This kit provides a rapid assay for nuclear condensation, a prominent hallmark of apoptosis.A highly permeable green-emitting dye for enhanced detection of apoptosis-induced chromatin condensation.
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Autophagie-Färbelösung, CYTO-ID®
Supplier: ENZO LIFE SCIENCES
CYTO-ID kann die Autophagozytose in lebenden Zellen und den autophagischen Fluss in lysosomal inhibierten lebenden Zellen mittels eines neuartigen Farbstoffs, der akkumulierte autophagische Vakuolen selektiv markiert, überwachen.
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GFP-CERTIFIED® FLUOFORTE® Calcium assay kit
Supplier: ENZO LIFE SCIENCES
The GFP-Certified™ FluoForte™ Calcium Assay kit provides a homogeneous fluorescence-based assay for detecting intracellular calcium mobilization across a broad spectrum of biological targets.
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Cellular senescence live cell analysis assay
Supplier: ENZO LIFE SCIENCES
Simple assay to quantify senescence-associated β-galactosidase activity, in live cells, using flow cytometry or fluorescent microscopy.
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ApoSENSOR™ ADP/ATP ratio assay kit
Supplier: ENZO LIFE SCIENCES
The assay is designed to detect ADP/ATP ratio. The changes in ADP/ATP ratios have been used to conveniently differentiate apoptosis from necrosis.
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DPPIV/CD26 assay kit for biological samples
Supplier: ENZO LIFE SCIENCES
The DPPIV/CD26 Assay kit for biological samples is a complete assay system designed to measure DPPIV activity in biological fluids such as plasma, serum, urine, and saliva. Uses for the kit include correlation of DPPIV activity with disease states or determination of the efficacy of DPPIV inhibitors administered in vivo.
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CV-Caspase 3 & 7 Detection kit
Supplier: ENZO LIFE SCIENCES
Based on the dye cresyl violet and the caspase 3/7 DEVD peptide recognition sequence, this kit allows the detection of caspases 3 and 7 in living cells by fluorescence microscopy.
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Auto-Ubiquitinylation Kit
Supplier: ENZO LIFE SCIENCES
This E3 ligase auto-ubiquitinylation kit enables proteins to be tested for ubiquitin E3 ligase activity through assessment of their ability to under auto-ubiquitinylation. Utilizing the first three steps in the ubiquitin cascade the kit facilitates ubiquitinylation of known or putative E3 ligase enzymes followed by Western blot analysis using the highly sensitive reagents provided or using antibodies to the specific protein of interest (user supplied). A high integrity ubiquitin E3 ligase enzyme is also provided for use as a positive control.
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Apoptosis and necrosis assay kit, NucView™488 RedDot™2
Supplier: Biotium
This NucView™488 and RedDot™2 Apoptosis and Necrosis Assay Kit contains NucView™488 caspase-3 substrate for detection of caspase-3/7 activity. The far-red dead-cell stain RedDot™2 is included for staining necrotic and late apoptotic cells that have compromised plasma membrane integrity. This kit provides a convenient tool for profiling apoptotic and necrotic cell populations by fluorescence microscopy or flow cytometry.
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TUNEL Assay-Kits
Supplier: Biotium
TUNEL (terminal deoxynucleotidyl transferase (TdT) mediated dUTP Nick-Enden-Markierung) ist hochselektiv für den Nachweis von apoptotischen Zellen, aber nicht von nekrotischen Zellen oder Zellen mit DNA-Strangbrüchen infolge von Bestrahlung oder medikamentöser Behandlung. In diesem Assay katalysiert das TdT-Enzym die Addition von markiertem dUTP an die 3'-Enden der gespaltenen DNA-Fragmente. Fluoreszenzfarbstoff-konjugiertes dUTP kann zum direkten Nachweis fragmentierter DNA durch Fluoreszenzmikroskopie oder Durchflusszytometrie verwendet werden.
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S-Glutathionylated Protein Detection Kit
Supplier: Cayman Chemical
A convenient method for the direct visualisation of S-glutathionylated proteins in whole (permeabilised) cells.
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CF® Dye Dextran 3500 MW, anionic and fixable
Supplier: Biotium
CF® dye labeled dextrans could be used as a fluorescent fluid-phase markers to study cell permeability, endocytosis, or mechanisms of biomolecular delivery. The dextran is 3,500 MW, and contains a formaldehyde-fixable free-amine group.
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Zytoplasmamembran-Markierungskits, CellBrite™
Supplier: Biotium
CellBrite-Kits wurden für die Markierung von Zellmembranen mit einem vorgelösten Membran-Fluoreszenzfarbstoff als optimierte Färbelösung entwickelt. Durch die Kombination von CellBrite Membran-Farbstoffen ist es möglich, mehrere Zellpopulationen mit unterschiedlichen Farben zu markieren. Dies ist vor allem für die Untersuchung von Zell-Zell-Interaktionen hilfreich.
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Epigenetics Screening Library
Supplier: Cayman Chemical
For screening a variety of epigenetic compounds.
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Apoptosis inducer set
Supplier: G-Biosciences
Apoptosis inducers are used for induction of apoptosis in cultured cells. The selection of apoptosis inducers include actinomycin D, camptothecin, cycloheximide, dexamethasone, doxorubicin and etoposide.
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HIF-1α transcription factor assay Kit
Supplier: Cayman Chemical
HIF-1α Transcription Factor Assay Kit is a sensitive, non radioactive method for detecting HIF-1α from whole cell lysates.
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p53 transcription factor assay kit
Supplier: Cayman Chemical
96-Well assay for measurement of p53.
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PPARδ transcription factor assay kit
Supplier: Cayman Chemical
A 96-well assay for measurement of PPARδ DNA binding activity.
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Neutrophil/Monocyte Respiratory Burst Assay Kit
Supplier: Cayman Chemical
Neutrophil/Monocyte Respiratory Burst Assay Kit provides PMA, dihydrorhodamine 123, and additional reagents necessary for inducing and quantifying a respiratory burst response in neutrophils and monocytes by flow cytometry. The assay can be performed on whole blood or on cells in various types of cell culture media. Because the assay reagents are not species-specific, this assay can be used in any species or cell type capable of producing a NADPH oxidase-dependent respiratory burst response.
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Membranpotenzial-Assay-Kit, FLIPR®
Supplier: Molecular Devices
Die FLIPR® Membranpotenzial-Assay-Kits erzeugen homogene fluoreszenzbasierte Rezepturen zur Beobachtung von Veränderungen des Membranpotenzials in Zusammenhang mit der Aktivierung von Ionenkanälen und Ionentransporterproteinen in Echtzeit.
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Maybridge Screening library
Supplier: THERMO SCIENTIFIC MAYBRIDGE
The Maybridge portfolio consists of high-quality hit-like, lead-like and drug-like compounds which can generate valuable data in screening programs. They are developed specifically to support the pharmaceutical drug discovery process.
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Glutathione Cell-Based Detection Kit (Blue Fluorescence)
Supplier: Cayman Chemical
For rapid and easy determination of GSH in whole cells.
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Prostaglandin Screening Library III
Supplier: Cayman Chemical
For A and J series prostaglandin analog screening.
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Nitrotyrosine IP Kit
Supplier: Cayman Chemical
For the capture and pulldown of nitrated proteins from cell and tissue lysates.
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Big blue
Supplier: AGILENT
These are used in all Big Blue rodents and cell lines.
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Glucose Uptake Cell-Based Assay Kit
Supplier: Cayman Chemical
A cell-based assay for studying modulators of cellular glucose uptake.
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Blaues IHC-Chromogen (HRP), HIGHDEF®
Supplier: ENZO LIFE SCIENCES
Das blaue IHC-Chromogen (HRP) ist für die Verwendung in Meerrettichperoxidase- (HRP)-basierten Immunfärbe- oder In-situ-Hybridisierungssystemen vorgesehen.
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Kolorimetrische DNMT-Tests für die Arzneimittelforschung
Supplier: ENZO LIFE SCIENCES
Das kolorimetrische DNMT-Nachweiskit für die Arzneimittelforschung stellt ein einfaches Verfahren zum Nachweis der DNA-Methylierungsaktivität oder -hemmung dar.
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LEADING LIGHT® Wnt cell line assay reagents
Supplier: ENZO LIFE SCIENCES
For use with the LEADING LIGHT® Wnt Reporter Cell Line.
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LEADING LIGHT® Wnt reporter cell line
Supplier: ENZO LIFE SCIENCES
Wnt reporter cell line for discovering Wnt pathway modulators.
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