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Zelltests

Zelltests

Zelluläre Assays waren schon immer ein leistungsstarkes Werkzeug im Forschungslabor. Sie sind äußerst vielseitig und können so konzipiert werden, dass sie nahezu jede zelluläre oder biochemische Funktion messen. Die empfindliche Detektion fluoreszierender Sonden unterstützt die Bewertung von Zellsignalisierung und Zelltodwegen, was für die schnelle Quantifizierung der Zellviabilität nützlich ist. Zelluläre Assays können verwendet werden, um die Auswirkungen verschiedener Behandlungen auf die Zellviabilität zu messen und toxische Effekte zu bewerten, die über eine Vielzahl von Mechanismen vermittelt werden.

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LEADING LIGHT® Wnt cell line medium pack

Supplier: Enzo Life Sciences

For use with the LEADING LIGHT® Wnt Reporter Cell Line.

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Epigenetics Screening Library

Epigenetics Screening Library

Supplier: Cayman Chemical

For screening a variety of epigenetic compounds.

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Apoptosis assay kit (Dual), NucView™ 488

Apoptosis assay kit (Dual), NucView™ 488

Supplier: Biotium

NucView® 488 and CF™dye (594, 640R and Texas RED) conjugated annexin V provides two apoptosis events, caspase-3 activity and phosphatidylserine (PS) translocation.

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Steady-Luc™ Firefly HTS assay kit

Steady-Luc™ Firefly HTS assay kit

Supplier: Biotium

Firefly luciferase is widely used as a transcriptional reporter gene for research and drug screening. The Steady-Luc™ HTS kit can be used with luminescence plate readers without sample injection systems or for high-throughput screening.

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ViaFluor® 647 live cell microtubule stain 1000X in DMSO

Supplier: Biotium

Stain microtubules in live cells with far-red fluorescence.

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Calcium assay kits, Fura-2 QBT™ Calcium kit

Calcium assay kits, Fura-2 QBT™ Calcium kit

Supplier: Molecular Devices

The Fura-2 QBT™ Calcium Kit eliminates the cause of data variability and reduces the number of steps compared to conventional wash protocols using Fura-2.

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Reporter assay kits, SpectraMax® Glo Steady-Luc™

Reporter assay kits, SpectraMax® Glo Steady-Luc™

Supplier: Molecular Devices

The SpectraMax® Glo Steady-Luc™ Reporter Assay Kit provides a highly sensitive assay for the quantitation of firefly luciferase expression in mammalian cells. The assay kit is optimised for Molecular Devices SpectraMax® microplate readers with a preconfigured protocol provided in SoftMax® Pro Software for simplified data acquisition and analysis.

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Green detection kit, MITO-ID®

Supplier: Enzo Life Sciences

MITO-ID® Green Detection Kit is a potent mitochondria-selective dye suitable for live, permeabilised and fixed cell staining.

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Catalase fluorometric detection kit

Supplier: Enzo Life Sciences

The Catalase fluorometric detection kit provides a higher throughput sensitive method for measuring catalase activity in just one hour. This is a homogeneous mix-and-read assay that enables you to monitor multiple time points for a kinetic read. Positive control is provided.

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Comet SCGE assay kit

Supplier: Enzo Life Sciences

COMET SCGE Assay measures DNA damage by fluorescently detecting the integrity of DNA liberated from cells embedded in low melting point agarose. Upon electrophoresis, fragmented DNA produces a characteristic "comet" shaped tail as small DNA fragments migrate in the gel more rapidly than in-tact genomic DNA.

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Reagents for Fluor-de-Lys®-Green HDAC fluorometric activity assay kit

Supplier: Enzo Life Sciences

Cellular assay, Fluor-de-Lys®-green substrate, in 50 mM solution in DMSO

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Exosome Isolation and Analysis Kit - Flow Cytometry, Plasma (CD9 / CD81), Abcam

Supplier: Abcam

Exosome Isolation and Analysis Kit - Flow Cytometry, Plasma (CD9/CD81) (ab267479) is a simple immunobead assay for isolation/detection of exosome, using a bead-bound anti-CD9 capture antibody and a fluorochrome conjugated anti-CD81 detection antibody.

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ViaFluor® 405 live cell microtubule stain 1000X in DMSO

Supplier: Biotium

Stain microtubules in live cells with blue fluorescence.

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LipidLaunch™ BODIPY SM-102 LNP Kit (Loadable)

Supplier: Cayman Chemical

Cayman’s LipidLaunch™ BODIPY SM-102 LNP kit (loadable) provides cargo-ready, empty, BODIPY-labelled SM-102 LNPs. LNPs are capable of rapid encapsulation of nucleic acids, without the need for incubation steps, enabling subsequent delivery to target cells or other downstream research applications. Also demonstrate very low toxicity compared to traditional lipid-based transfection methods.

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Red detection kit (GFP-CERTIFIED®), MITO-ID®

Supplier: Enzo Life Sciences

MITO-ID® Red Detection Kit (GFP-CERTIFIED®) is a photostable, non-toxic and selective mitochondrial dye that stains live, detergent permeabilised and aldehyde fixed cells. This kit is specifically designed for use with GFP expressing cell lines, as well as cells expressing blue, cyan or yellow fluorescent proteins (BFP's, CFP's, YFP's).

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Färbelösungen, Ghost Dye™

Färbelösungen, Ghost Dye™

Supplier: Tonbo Biosciences

Ghost Dye Färbelösungen ermöglichen eine klare Unterscheidung von lebenden und toten Zellen für eine präzisere durchflusszytometrische Analyse und sind besonders nützlich für intrazelluläre Färbeprotokolle.

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