Zelltests
Zelluläre Assays waren schon immer ein leistungsstarkes Werkzeug im Forschungslabor. Sie sind äußerst vielseitig und können so konzipiert werden, dass sie nahezu jede zelluläre oder biochemische Funktion messen. Die empfindliche Detektion fluoreszierender Sonden unterstützt die Bewertung von Zellsignalisierung und Zelltodwegen, was für die schnelle Quantifizierung der Zellviabilität nützlich ist. Zelluläre Assays können verwendet werden, um die Auswirkungen verschiedener Behandlungen auf die Zellviabilität zu messen und toxische Effekte zu bewerten, die über eine Vielzahl von Mechanismen vermittelt werden.
Calcium assay kits, FLIPR® Calcium 4 assay kit
Supplier: Molecular Devices
The FLIPR® Calcium 4 Assay Kit provides a fast, simple and reliable fluorescence-based assay for detecting changes in intracellular calcium.
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Cyclic-AMP fluorescent assay kits, CatchPoint™
Supplier: Molecular Devices
CatchPoint™ cAMP Fluorescent Assay Kit measures cAMP levels via a competitive immunoassay format. Cell signaling via G protein-coupled receptors (GPCRs) can be assessed by monitoring the downstream effectors calcium or Cyclic AMP (cAMP). Monitoring levels of cAMP, a second messenger produced in response to activation of adenylate cyclase, is one of the most common ways to screen for agonists and antagonists of GPCRs.
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ROS-ID™ superoxide detection kit
Supplier: Enzo Life Sciences
This kit is designed to directly monitor real time superoxide production in live cells using fluorescence microscopy and/or flow cytometry.
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CFDA SE Cell proliferation assay kit
Supplier: Biotium
Cell proliferation dyes diffuse passively into cells and covalently label intracellular proteins, resulting in long term cell labelling. They are non-fluorescent until they are hydrolysed by intracellular esterase's. The dyes then react with intracellular amines forming fluorescent conjugates that are retained in the cell. Immediately after staining, a single, bright fluorescent population will be detected by flow cytometry. With each cell division, daughter cells inherit roughly half of the fluorescent label, allowing the number of cell divisions that occur after labelling to be detected by the appearance of successively dimmer fluorescent peaks on a flow cytometry histogram compared to cells analysed immediately after staining. Staining is formaldehyde fixable.
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PRC Pathway Modulator Set Abcam
Supplier: Abcam
PRC Pathway Modulator Set (ab284177) is part of the multiplex kits range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.
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LipidLaunch™ LNP Apparent pKa Assay Kit (TNS Method)
Supplier: Cayman Chemical
Caymans LipidLaunch™ LNP Apparent pKa Das Testkit (TNS-Methode) bietet eine robuste und benutzerfreundliche Plattform zur Bestimmung des scheinbaren/globalen p-Werts.Ka von formulierten LNPs.
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Firefly luciferase assay kit (Lyophilised)
Supplier: Biotium
This kit is designed to measure firefly luciferase activity in transfected cell reporter assays.
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Gelbes IHC-Chromogen (HRP), HIGHDEF®
Supplier: Enzo Life Sciences
Das gelbe IHC-Chromogen (HRP) ist für die Verwendung in Meerrettichperoxidase(HRP)-basierten Immunfärbe- oder In-situ-Hybridisierungssystemen vorgesehen.
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Hydrogen peroxide chemiluminescent detection Kit
Supplier: Enzo Life Sciences
For the quantitative determination of hydrogen peroxide in culture supernatants and cell lysates from any species.
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Green detection kit, LYSO-ID®
Supplier: Enzo Life Sciences
LYSO-ID® Green Detection Kit is an acidic organelle-selective dye specially designed to use with RFP-expressing cell lines, as well as cells expressing blue, cyan or orange fluorescent proteins (BFPs, CFPs, OFPs).
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Cell viability assay kits, EarlyTox™ Caspase-3/7 and Caspase-3/7-D NucView™ 488 Assay Kits
Supplier: Molecular Devices
EarlyTox™ Cell Viability Assay Kits are a family of fluorescence-based reagents for the assessment of cell viability, cell proliferation, and various apoptosis events using mammalian cells. Optimised for use with microplate readers, these assay kits employ a no-wash, homogeneous assay protocol that enables characterisation of a full concentration-response profile of test compounds.
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Ubiquitin conjugating kit (HeLa lysate-based)
Supplier: Enzo Life Sciences
1. Ubiquitinylation of specific endogenous HeLa lysate proteins, followed by their immediate detection/analysis using antibodies to the protein/s of interest, indicating a particular protein is a substrate for the ubiquitin-proteasome pathway. 2. Ubiquitinylation of proteins of interest contained in exogenously added expression culture/cell extracts or tissue lysates/extracts, followed by their immediate detection/analysis or isolation/purification for use in subsequent experiments. 3. Modification of proteins using ubiquitin derivatives or mutants for improved detection/analysis or investigation of alternative (non-proteasomal) ubiquitin related pathways in subsequent experiments. For example, biotinylated-ubiquitin (Prod. No. BML-UW8705), for sensitive detection
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SOD activity kit
Supplier: Enzo Life Sciences
ELS offers a large collection of superoxide dismutase products for oxidative stress research.
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Ubiquitin activating kit
Supplier: Enzo Life Sciences
Ubiquitin Activating Kit for activation of ubiquitin for use in ubiquitinylation experiments. The kit provides the means of generating thioester linked, activated ubiquitin-E1 conjugates, utilising the first step in the ubiquitin cascade, for investigation of ubiquitin activation, subsequent ubiquitin transfer to/interaction with E2 conjugating enzymes, and their use in the ubiquitinylation of E3 ligases and target substrate proteins. Ubiquitin antibody provided allows high sensitivity detection of ubiquitin and ubiquitin-conjugates.
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p70 S6K activity kit
Supplier: Enzo Life Sciences
The p70 S6K activity kit is an efficient assay that utilizes little sample volume. This kit gives you the ability to an end-point or kinetic assay read-out in a convenient 96-well plate based assay and offers easy sample handling protocols. The assay also offers a high signal to background ratio.
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Calcium assay kit
Supplier: Cayman Chemical
For the determination of total calcium concentration in a variety of biological samples, as well as tissue homogenates and cell lysates.
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