Zelltests
Zelluläre Assays waren schon immer ein leistungsstarkes Werkzeug im Forschungslabor. Sie sind äußerst vielseitig und können so konzipiert werden, dass sie nahezu jede zelluläre oder biochemische Funktion messen. Die empfindliche Detektion fluoreszierender Sonden unterstützt die Bewertung von Zellsignalisierung und Zelltodwegen, was für die schnelle Quantifizierung der Zellviabilität nützlich ist. Zelluläre Assays können verwendet werden, um die Auswirkungen verschiedener Behandlungen auf die Zellviabilität zu messen und toxische Effekte zu bewerten, die über eine Vielzahl von Mechanismen vermittelt werden.
Nuclear Green™ DCS1
Supplier: AAT Bioquest
Our Nuclear Green™ DCS1 is a fluorogenic, DNA-selective and cell-impermeant dye for analysing DNA content in dead, fixed or apoptotic cells.
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LysoBrite™ Orange
Supplier: AAT Bioquest
Lysosomes are cellular organelles which contain acid hydrolase enzymes to break up waste materials and cellular debris.
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Cell Explorer™ Live Cell Labelling Kit *Blue Fluorescence*
Supplier: AAT Bioquest
Our Cell Explorer™ fluorescence imaging kits are a set of tools for labelling cells for fluorescence microscopic investigations of cellular functions.
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Live or Dead™ Cell Viability Assay Kit *Red/Blue Dual Fluorescence*
Supplier: AAT Bioquest
This Live or Dead™ Cell Viability Assay Kit uses two fluorescent indicators: Cellbrite™ Red (Ex/Em = 613/631 nm) for labelling viable cells and a cell-impermeable DNA-binding dye Nuclear Blue™ DCS1 (Ex/Em = 360/450 nm) for labelling dead cells with damaged membranes.
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Helix Fluor™ 545 Succinimidyl Ester
Supplier: AAT Bioquest
FAM, TET, VIC, JOE, NED, TAMRA and ROX fluorescent dyes are frequently used for labelling oligos.
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Live or Dead™ Cell Viability Assay Kits *Green/Red Dual Fluorescence*
Supplier: AAT Bioquest
This Live or Dead™cell viability uses two fluorogenic indicators: calcein AM for viable cells and a cell-impermeable DNA-binding dye for the cells with compromised membranes.
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MitoLite™ Red CMXRos
Supplier: AAT Bioquest
MitoLite™ red CMXRos is chemically same to the MitoTracker™ Red CMXRos (ThermoFisher).
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Cell Meter™ Intracellular NADH/NADPH Fluorescence Imaging Kit *Deep Red Fluorescence*
Supplier: AAT Bioquest
The detection of intracellular dihydronicotinamide adenine dinucleotide NADH and its phosphate ester NADPH is important for disease diagnostics and drug discovery.
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PhosphoWorks™ Fluorimetric ATP Assay Kit
Supplier: AAT Bioquest
Adenosine triphosphate (ATP) plays a fundamental role in cellular energetics, metabolic regulation and cellular signaling.
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Live or Dead™ Fixable Dead Cell Staining Kit *NIR Fluorescence*
Supplier: AAT Bioquest
Our Live or Dead™ Fixable dead cell staining kits are a set of tools for labelling cells for fluorescence microscopic investigations of cellular functions.
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Bodi Fluor™ R6G NHS Ester [equivalent to Bodipy® R6G, NHS Ester]
Supplier: AAT Bioquest
Bodi Fluor™R6G, NHS ester is the same molecule to Bodipy® R6G, NHS ester (Bodipy® is the trademark of Invitrogen).
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G-Biosciences Calcein Release Assay (Cell Viability Assay)
Supplier: G-Biosciences
Calcein AM Cell Viability Assay can be easily adapted to various fluorescence setups such as microplate assays, fluorescence microscope and flow cytometry. The assay is useful for various studies such as cell viability, cell adhesion, chemotaxis, multidrug resistance, apoptosis and cytotoxicity.
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Firefly luciferase assay kit 2.0
Supplier: Biotium
This kit is designed to measure Renilla luciferase activity in transfected cell reporter gene assays.
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BCA protein assay kit
Supplier: Merck Millipore (Novagen)
BCA Protein Assay is a simple and reliable protein quantification method. It can be used to determine protein concentration in the range of 20-2000 µg/ml in either a standard assay or microassay configuration.
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Firefly and Renilla Luciferase single tube Assay
Supplier: Biotium
This kit is designed to measure Firefly and Renilla luciferase activities in transfected cell reporter assays. Firefly luciferase is widely used as a reporter for studying gene regulation and function, and for pharmaceutical screening. It is a very sensitive genetic reporter due to the absence of endogenous luciferase activity in mammalian cells or tissues. Renilla luciferase has been used as a reporter gene for studying gene regulation and function in vitro and in vivo. It commonly is used in multiplex transcriptional reporter assays or as a normalising transfection control for firefly luciferase assays.
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Alamar blue cell viability assay
Supplier: G-Biosciences
AlamarBlue Cell Viability Assay reagent quantitatively measures the proliferation of mammalian cell lines, bacteria and fungi. The dye incorporates an oxidation-reduction (REDOX) indicator that both fluoresces and change colour in response to the chemical reduction of growth medium due to cell growth.
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