Sie haben gesucht nach: Proteinkonjugate und Markierungen

Supplier: Thermo Fisher Scientific

The NHS-Azide and NHS-Phosphine Reagents are amine-reactive compounds for derivatising primary amines of proteins or amine-coated polymer surfaces. Once a protein or surface is azide- or phosphine-labelled, the two components are mixed for effective and stable conjugation. Phosphine groups react with azides via a Staudinger reaction to produce an aza-ylide intermediate that is trapped to form a stable, covalent amide bond. Because phosphines and azides are absent from biological systems, there is minimal background labelling of macromolecules found in cells or lysates.

Supplier: Thermo Fisher Scientific

EZ-Link® Phosphine-PEG3-Biotin is a versatile biotinylation reagent for labeling azide-containing molecules, which enables biotin-based detection and affinity purification of molecules via Staudinger ligation strategies.

Supplier: Thermo Fisher Scientific

Pierce™ FITC antibody labeling kit is a multi-component kit containing a high performance derivative of fluorescein dye, activated for easy and reliable labeling of antibodies, proteins and other molecules for use as fluorescent probes.

Supplier: Thermo Fisher Scientific

Photoactivatable Biotin, Biotin-LC-ASA , Psoralen-PEG3-Biotin and TFPA-PEG3-Biotin each contain a photoactivatable group. When exposed to UV light, they become activated and insert non specifically into nearby molecules. These reagents may be used to label proteins and peptides, but they are also useful in labeling DNA, RNA and other molecules that do not contain any readily reactive functional groups. Psoralen-PEG3-Biotin contains a reactive psoralen group and is
designed to efficiently label nucleic acids. The psoralen group intercalates into the helix of DNA, allowing it to label efficiently and selectively. The psoralen can also stack along with the bases of single-stranded DNA or RNA increasing labeling efficiency and selectivity.

Supplier: Thermo Fisher Scientific

Cross linkers are used for the identification of near-neighbour protein relationships and ligand-receptor interactions. Homobifunctional, amine-reactive NHS esters or imidates, and heterobifunctional, amine-reactive, photoactivatable phenyl azides are the most commonly used crosslinkers for these applications.

Supplier: Thermo Fisher Scientific

Das Pierce™ Antikörper-Biotinylierungs-Kit für IP bietet Biotinylierungs-Reagenzien speziell für die Markierung von primären Antikörpern in Immunpräzipitations-Anwendungen.

Supplier: Thermo Fisher Scientific

Das Thermo Scientific™ Pierce™ MS-kompatible magnetische IP-Kit (Protein A/G) bietet massenspektrometriefreundliche Reagenzien und ein optimiertes Protokoll, um eine hocheffektive und effiziente Immunpräzipitation und Co-Immunpräzipitation von Zielantigenen vor der LC-MS-Analyse zu ermöglichen.

Supplier: Thermo Fisher Scientific

The iodoTMTsixplex™ labelling reagents enable concurrent identification and multiplexed quantitation of proteins in different samples using tandem mass spectrometry.

Supplier: AAT BIOQUEST

XFD488 is manufactured by AAT Bioquest, and it has the same chemical structure of Alexa Fluor® 488 (Alexa Fluor® is the trademark of ThermoFisher).

Supplier: AAT BIOQUEST

Es ist mit einer Vielzahl von Probenmaterialien kompatibel, einschließlich bakterieller künstlicher Chromosomen-DNA (BAC), menschlicher genomischer DNA, gereinigten PCR-Produkten sowie superspiralisierter und linearisierter Plasmid-DNA.

Supplier: AAT BIOQUEST

CytoWatch™ Wash-free fluorescence cell imaging buffer *10X* is a ready-to-use buffer optimised for fluorescence cell imaging.

Supplier: AAT BIOQUEST

iFluor® 532 WGA conjugate, like Alexa Fluor® 532 WGA conjugate, retains its ability to bind to sialic acid and N-acetylglucosaminyl residues, enhancing its utility in fluorescence imaging and analysis for various scientific investigations.

Supplier: AAT BIOQUEST

iFluor® 568 Tris-NTA Chelator is used as a sensitive fluorescent probe for detecting polyhistidine-labelled proteins in combination with the addition of a heavy metal ion (such as Ni²⁺) in cells, solution and solid surfaces.

Supplier: AAT BIOQUEST

iFluor 647-Tris NTA compound is used as a sensitive fluorescent probe for detecting polyhistidine-labelled proteins in cells, solution and solid surfaces.

Supplier: AAT BIOQUEST

Microscale optimised for labeling 50 µg antibody per reaction. ReadiLink™ Rapid mFluor™ Violet 610 antibody labelling kits provide a quick and convenient method to label antibodies, proteins (<10 kDa), or other amine-containing biomolecules.

Supplier: AAT BIOQUEST

DY677 CEP (DyLight 677 phosphoramidite) is an excellent replacement to the commonly used Cy5.5 phosphoramidite.

Supplier: AAT BIOQUEST

APC-iFluor® 700 Tandem ist ein hervorragender Ersatz für APC-Alexa Fluor® 700 Tandem, da beide nahezu identische Spektren aufweisen.

Supplier: AAT BIOQUEST

APC-Cy7 ist eine beliebte Farbe, die in der Durchflusszytometrie verwendet wird.

Supplier: AAT BIOQUEST

Our Buccutite™crosslinking technology provides the most convenient and effective crosslinking method to link two biomolecules with a high conjugation yield.

Supplier: AAT BIOQUEST

ReadiLink™ Rapid antibody labelling kits, designed for production scale, provide a convenient and efficient method for labelling large volumes of antibodies with XFD dyes (equivalent to Alexa Fluor®).

Supplier: AAT BIOQUEST

ReadiLink™ Rapid antibody labelling kits, designed for production scale, provide a convenient and efficient method for labelling large volumes of antibodies with XFD dyes (equivalent to Alexa Fluor®).

Supplier: AAT BIOQUEST

ReadiLink™ Rapid antibody labelling kits, designed for production scale, provide a convenient and efficient method for labelling large volumes of antibodies with our superior iFluor® dyes.

Supplier: AAT BIOQUEST

ReadiLink™ xtra rapid antibody labelling kits require essentially only two simple mixing steps without a column purification needed.

Supplier: AAT BIOQUEST

XFD488 is manufactured by AAT Bioquest, and it has the same chemical structure of Alexa Fluor® 488 (Alexa Fluor® is the trademark of ThermoFisher).

Supplier: AAT BIOQUEST

Oxidation of MitoROS™ 580 by superoxide generates red fluorescence.

Supplier: AAT BIOQUEST

AAT Bioquest's iFluor® dyes are developed for labelling proteins, in particular, antibodies.

Supplier: AAT BIOQUEST

AAT Bioquest's mFluor™ dyes are developed for flow cytometry-focused applications.

Supplier: AAT BIOQUEST

AAT Bioquest's mFluor™ dyes are developed for flow cytometry-focused applications.

Supplier: AAT BIOQUEST

XFD647, manufactured by AAT Bioquest, has an identical chemical structure to Alexa Fluor® 647, a trademark of ThermoFisher.

Supplier: Thermo Fisher Scientific

Carboxyl groups, in the form of carboxy termini, aspartate residues or glutamate residues, can be targeted for biotin labeling using amine-derivatised biotin molecules. This reaction is mediated by a class of crosslinkers known as carbodiimides and results in the formation of an amide bond. The reaction with EDC, the most common carbodiimide crosslinker, is generally performed in an MES buffer at pH 4,5 to 5 and requires just minutes to complete.