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Sie haben gesucht nach: Proteinkonjugate und Markierungen

Proteinkonjugate und Markierungen

Protein linking and labelling systems provide a convenient means for attaching fluorescent labels or biotins to antibodies and larger proteins. These kits are ideal for use with multiple applications, including flow cytometry, fluorescent microscopy, immunohistochemistry, primary detection, ELISAs, immunocytochemistry, and FISH. These systems are available with a variety of dye colours, biotin, fluorescent agents, and all the components needed to perform multiple conjugations and purifications. Protein labelling follows a simple protocol of reaction, separation, and then use.

Carboxyl coupling resin

Carboxyl coupling resin

Supplier: G-Biosciences

Carboxyl coupling resin consists of 6% cross-linked agarose with covalently linked diaminodipropylamine (DADPA) to generate a free primary amine at the end of a long spacer arm. Molecules, including proteins, peptides and nucleic acids, are covalently coupled to the free primary amines, and the stable columns are ideal for affinity purification of antibodies and other interacting partners.

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GFP-Certified™ FluoForte™ Reagent

Supplier: ENZO LIFE SCIENCES

Calcium dye

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Bio-7-dATP

Bio-7-dATP

Supplier: ENZO LIFE SCIENCES

Bio-7-dATP (Biotin-7-2’-deoxyadenosine-5’-triphosphate) can replace dATP in reactions in which it serves as a substrate for E. coli DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase.

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CGH labeling kit, CYTAG®

Supplier: ENZO LIFE SCIENCES

Comparative genomic hybridisation (CGH) is a powerful diagnostic tool for detecting DNA copy number gains and losses associated with chromosome abnormalities. CGH provides an understanding of genetic disorders, cancers and other genomic aberrations.

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Fluorescein-12-UTP

Supplier: ENZO LIFE SCIENCES

Fluorescein-12-UTP is ideal for in vitro transcription reactions catalysed by T3, T7, or SP6 RNA polymerases and reverse transcriptase to produce fluorophore-labeled RNA transcripts. RNA-based probes can be used to identify complementary sequences by in situ hybridisation to fixed cells and tissues by direct or indirect fluorescence detection or by membrane hybridisation procedures.

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Red 650 dUTP

Supplier: ENZO LIFE SCIENCES

Red 650 [Cyanine-5E] dUTP is a cyanine-5 derivative with enhanced spectral properties. This nucleotide analog can replace TTP in reactions in which it serves as a substrate for E.coli DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase. Fluorescently labeled probes can be prepared with this fluorescent nucleotide by a variety of methods including nick translation, cDNA labelling and 3'-end labelling.

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NEDDylation kit

Supplier: ENZO LIFE SCIENCES

This kit provides the means of generating a thioester-linked NEDD8-conjugated E2 enzyme, utilizing the first two steps in the NEDD8 cascade, for use in the NEDDylation of E3 ligases and target substrate proteins (user supplied). A NEDD8-specific antibody is provided for detection of NEDDylated proteins via SDS-PAGE and Western blotting.

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Phosphotyrosine detection set

Supplier: ENZO LIFE SCIENCES

This kit offers a set of seven different MAbs specific for tyrosine phosphorylation sites. Phosphorylation patterns in a given cell extract may differ when probed with different antibodies due to sequence specificity. Recognition therefore depends on two criteria: Phosphorylation and surrounding aa motif. If one of the two criteria is not fulfilled, the antibody will not detect the phosphorylation site. Specificity has been determined by epitope mapping using degenerated phosphopeptide libraries.

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Bio-N⁶-ddATP

Bio-N⁶-ddATP

Supplier: ENZO LIFE SCIENCES

Bio-N⁶-ddATP (Biotin-N⁶-(2’,3’-dideoxy)adenosine-5’ triphosphate) can be used for 3’-end labelling of single-stranded or double-stranded DNA. It is especially useful as a substrate for terminal transferase for the 3’-terminal addition of a single Bio-N⁶-ddAMP. It is also a substrate for Taq DNA Polymerase and other DNA polymerases.

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Phosphothreonine detection set

Supplier: ENZO LIFE SCIENCES

This kit offers a set of three different monoclonal antibodies specific for threonine phosphorylation sites. Phosphorylation patterns in a given cell extract may differ when probed with different antibodies due to sequence specificity. Recognition therefore depends on two criteria: Phosphorylation and surrounding aa motif. If one of the two criteria is not fulfilled, the antibody will not detect the phosphorylation site.

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Allylamine-dUTP

Allylamine-dUTP

Supplier: ENZO LIFE SCIENCES

Allylamine-2’-deoxyuridine-5’-triphosphate (Allylamine-dUTP) can replace TTP in reactions where it serves as a substrate for E.coli DNA polymerase 1 (holoenzyme and Klenow fragment), terminal deoxynucloetide transferase, T4 and Taq DNA polymerases, and reverse transcriptases (from ALV and MLV).

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Nanobody labelling kits, Mix-n-Stain™

Nanobody labelling kits, Mix-n-Stain™

Supplier: Biotium

The simplicity and convenience of Mix-n-Stain™ labelling of nanobodies with fluorescent CF® dyes or biotin.

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CF® Reaktive Farbstoffe und Protein Etiketten Kits

CF® Reaktive Farbstoffe und Protein Etiketten Kits

Supplier: Biotium

Biotium bietet eine Auswahl an Formaten mit Reaktivfarbstoffen zum Markieren von Proteinen, Nukleinsäuren und anderen Biomolekülen mit Fluoreszenzfarbstoffen an. CF®-Farbstoffe haben Vorteile in Bezug auf Helligkeit und Photostabilität im Vergleich zu anderen ähnlichen kommerziellen Farbstoffen. CF®-Farbstoffe sind in einer Reihe von Formaten für CF®-Farbstofffarben erhältlich, die das sichtbare und nahe Infrarotspektrum abdecken.

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Stabiliser for proteins and standards, SeramunStab® Stpur

Supplier: Apollo Scientific

Liquid product colourless to slightly yellow or greenish odourless pH 7,3 to 7,5 boiling point 104 °C. Stabiliser for controls and protein standards.

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CF®DI Hydrazide

CF®DI Hydrazide

Supplier: Biotium

CF®DI dye hydrazides react with molecules containing aldehyde or ketone groups to form a hydrazone bond. The dyes are size- and charge-matched and do not significantly alter the molecular weight or pI of the labeled proteins. They are used for detection of protein carbonylation by fluorescence 2D-DIGE.

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Immobilisation kit, SDC™ (Steroid/Drug/Compound)

Immobilisation kit, SDC™ (Steroid/Drug/Compound)

Supplier: G-Biosciences

SDC™ (Steroid/Drug/Compound) Immobilisation Kit is designed for the immobilisation of steroids, drugs and chemical compounds that lack primary amines, sulfhydryls, carbonyls and other common coupling groups to a solid-phase agarose support for the use in affinity purification. The kit uses Immobilised DADPA (diaminodipropylamine) resin to bind steroids, drugs and chemicals through their active hydrogens.

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HRP - conjugate stabiliser, SeramunStab® STM

Supplier: Apollo Scientific

Liquid product orange to red odourless pH 6,4 to 6,6. Stabiliser for conjugates with monomeric HRP. Preservation of very low conjugate concentrations.

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Peroxidase anti-peroxidase (PAP) soluble complexes

Supplier: Rockland Immunochemicals

Peroxidase anti-peroxidase soluble complexes are used for peroxidase enzymatic labelling experiments.

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Reactive dye conjugates

Supplier: Biotium

Reactive dyes containing an amine functional group.

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D-luciferin luminescent substrates

Supplier: Biotium

Luciferin is a substrate for the enzyme firefly luciferase.

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Photoreactive dyes and reagents

Supplier: Biotium

INA (5-iodonaphthyl-1-azide) is a lipophilic photoreactive probe, which has been used to selectively label membrane-embedded cysteine residues of proteins.

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Streptavidin, R-phycoerythrin (R-PE) conjugate

Supplier: Biotium

Streptavidin conjugates can be used to detect biotinylated molecules with extremely high sensitivity. They are commonly used as secondary reagents to detect biotinylated primary probes such as antibodies in flow cytometry, Western blotting, immunocytochemistry and other applications.

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CF® Dye Alkyne

CF® Dye Alkyne

Supplier: Biotium

CF® Dye alkynes react with azide groups via copper-catalysed bioorthogonal cycloaddition. Can be used to fluorescently detect or label azide groups on target molecules. CF® Dye alkynes can also be used as building blocks to form fluorescent polymers.

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Small ligand labelling kits, Mix-n-Stain™ CF® Dye

Small ligand labelling kits, Mix-n-Stain™ CF® Dye

Supplier: Biotium

Easily label SNAP-tag®, CLIP-tag™, HaloTag®, or other ligands with fluorescent dyes, no purification needed. Cell membrane-permeant and impermeant dye options for intracellular or surface targets.

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Glogos II autorad markers

Supplier: AGILENT

Luminescent peel-off stickers are used for accurately marking and aligning autoradiographs.

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Gold 525 dUTP

Supplier: ENZO LIFE SCIENCES

Gold 525 [5(6)-Carboxyrhodamine 6G] dUTP can replace TTP in reactions in which it serves as a substrate for E. coli DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase. Fluorescently labelled probes can be prepared with this fluorescent nucleotide by a variety of methods including nick translation, random prime labelling, cDNA labelling and 3’-end labelling. Probes generated by these methods are suitable for use for the identification of specific sequences by in situ hybridization procedures on fixed cells and tissues by direct fluorescence detection. Gold 525 dUTP can also be used for multicolor fluorescence labelling.

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BIOARRAY™ Low Input RNA Amplification with Biotin Labeling System

Supplier: ENZO LIFE SCIENCES

The BIOARRAY™ Low Input RNA Amplification with Biotin Labeling System provides an optimised protocol and reagents for the production of biotin-labeled antisense RNA (aRNA) from total cellular RNA samples in less than 24 hours for array analysis.

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100-reaction single-round RNA amplification and Biotin labeling system

Supplier: ENZO LIFE SCIENCES

Improved data quality and analysis results through greater biotin incorporation.

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Red 580 dUTP (lyophilised)

Supplier: ENZO LIFE SCIENCES

Red 580 dUTP (lyophilised)

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Fluor phosphine reagents, DyLight™, Pierce™

Fluor phosphine reagents, DyLight™, Pierce™

Supplier: Thermo Fisher Scientific

DyLight™ Fluor phosphine reagents are phosphine-activated fluorescent dyes for specific labeling and detection of azide-tagged molecules, which enables use of fluorescence imaging in metabolic labeling strategies.

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