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You searched for: Proteinkonjugate und Markierungen

Proteinkonjugate und Markierungen

Protein linking and labelling systems provide a convenient means for attaching fluorescent labels or biotins to antibodies and larger proteins. These kits are ideal for use with multiple applications, including flow cytometry, fluorescent microscopy, immunohistochemistry, primary detection, ELISAs, immunocytochemistry, and FISH. These systems are available with a variety of dye colours, biotin, fluorescent agents, and all the components needed to perform multiple conjugations and purifications. Protein labelling follows a simple protocol of reaction, separation, and then use.

ZnAF-2 DA

Supplier: ENZO LIFE SCIENCES

Fluorescent probe for Zn²⁺. Cell permeable.

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Organelle-ID™ RGB reagent I

Supplier: ENZO LIFE SCIENCES

Mixture of fluorescent dyes for detection of lysosomes, mitochondria and nucleic acids

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Aqua 431 dUTP (lyophilised)

Supplier: ENZO LIFE SCIENCES

Aqua 431 dUTP (lyophilised)

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Phosphoserine detection set

Supplier: ENZO LIFE SCIENCES

The kit offers a set of six different MAbs specific for serine phosphorylation sites. Phosphorylation patterns in a given cell extract may differ when probed with different antibodies due to sequence specificity. Recognition therefore depends on two criteria: phosphorylation and surrounding aa motif. If one of the two criteria is not fulfilled, the antibody will not detect the phosphorylation site. Specificity has been determined by epitope mapping using degenerated phosphopeptide libraries. Clone 16B4 strongly interacts with pSK and pSP motifs, thus specifically recognizes substrates of MAP/SAP Kinases and CDC kinases.Clones 1C8, 4A3, 4A9, 4H4 and 7F12 interact with epitopes containing phosphoserine in the context of positive or neutral aa (e.g. substrates of PKA, PKB, PKC, PKG etc.) Though revealing a similar pattern in epitope mapping using phosphopeptide libraries, these antibodies interact with different sets of phosphoproteins in whole cell lysates.

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Green 496 dUTP

Supplier: ENZO LIFE SCIENCES

Green 496 [5-Fluorescein] dUTP can replace TTP in reactions in which it serves as a substrate for E. coli DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase.

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Green 496 dUTP (lyophilized)

Green 496 dUTP (lyophilized)

Supplier: ENZO LIFE SCIENCES

Green 496 dUTP (lyophilised)

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Wheat germ agglutinin (WGA) conjugates

Wheat germ agglutinin (WGA) conjugates

Supplier: Biotium

A wheat germ agglutinin (WGA) lectin labelled with Biotium’s CF® Dyes or HRP. It is a carbohydrate-binding lectin that has high affinity for sialic acid and N-acetylglucosamine moieties of glycoproteins. As a result, WGA conjugates will label yeast bud scars and the cell membrane of gram-positive bacteria and mammalian cells.

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ExoBrite™ True EV-Membranfarbstoffe

Supplier: Biotium

Lipophile Membranfarbstoffe, die speziell für die EV-Forschung entwickelt wurden und die beste Wahl für die allgemeine Pan-EV-Färbung sind.

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Coelenterazine Substrates for Renilla Luciferase and Apoaequorin

Coelenterazine Substrates for Renilla Luciferase and Apoaequorin

Supplier: Biotium

Coelenterazine and its analogs are luminescent enzyme substrates for apoaequorin and Renilla luciferase.

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Antikörper-Markierungs-Kits, Mix-n-Stain™ FITC

Antikörper-Markierungs-Kits, Mix-n-Stain™ FITC

Supplier: Biotium

Das Mix-n-Stain™ FITC Antikörper-Markierungs-Kit nutzt eine Technologie, die eine schnelle Antikörpermarkierung mit einem grün fluoreszierenden Farbstoff (FITC) ohne Aufreinigungsschritt ermöglicht.

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CF™ dye streptavidin and monoclonal mouse anti-biotin conjugates

Supplier: Biotium

CF™ dyes are superior to other fluorescent dyes for protein labelling due to advantages in brightness, photostability and water solubility.

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HRP-conjugate stabiliser, SeramunStab® STA2

Supplier: Apollo Scientific

Liquid product red odourless pH-value 7,3 to 7,5 Boiling point 102 °C. Stabiliser for a broad range of HRP conjugates.

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CF® Dye cAMP

Supplier: Biotium

Fluorescently labelled cAMP analogs that can be used to probe cAMP receptors.

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Tyramide amplification buffer plus

Tyramide amplification buffer plus

Supplier: Biotium

An optimized buffer with enhanced sensitivity for tyramide signal amplification of immunostaining in cells or tissue sections.

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R-Phycoerythrin (RPE)

R-Phycoerythrin (RPE)

Supplier: Columbia Biosciences

Many applications and instruments were developed specifically for R-phycoerythrin.

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Gold 525 dUTP (lyophilised)

Supplier: ENZO LIFE SCIENCES

Gold 525 dUTP (lyophilised)

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Bio-16-ddUTP, tetralithium salt

Bio-16-ddUTP, tetralithium salt

Supplier: ENZO LIFE SCIENCES

Bio-16-ddUTP (Biotin-16-2’,3’-dideoxyuridine-5’-triphosphate) can be used for 3’-end labelling of single-stranded or double-stranded DNA. It is especially useful as a substrate for terminal transferase for the 3’-terminal addition of a single Bio-16-ddUMP. Biotin-labelled oligonucleotides produced by this reaction are suitable as hybridisation probes and nucleic acid array assays. It is also a substrate for Taq DNA polymerase and other DNA polymerases.

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Fluorescein-12-ddUTP

Fluorescein-12-ddUTP

Supplier: ENZO LIFE SCIENCES

Fluorescein-12-ddUTP can be used for 3’-end labeling of oligodeoxynucleotides using terminal transferase. It also serves as a substrate for E.coli DNA Polymerase I (holoenzyme and Klenow fragment), T4 and T7 DNA polymerases, Taq DNA polymerase and reverse transcriptase. Oligonucleotide probes labelled with fluorescein-12-ddUTP can be detected by direct fluorescence detection (green fluorescence) or by methods using horseradish peroxidase- or alkaline phosphatase-linked anti-fluorescein antibodies.

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Organelle-ID™ RGB reagent II

Supplier: ENZO LIFE SCIENCES

Mixture of fluorescent dyes for detection of lysosomes, endoplasmic reticulum and nucleic acids

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Gold 550 dUTP

Supplier: ENZO LIFE SCIENCES

Gold 550 [Cyanine-3E] dUTP is a cyanine-3 derivative with enhanced spectral properties. This nucleotide analogue can replace TTP in reactions in which it serves as a substrate for E.coli DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase.

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Reactive biotin compounds

Supplier: Biotium

Biotium offers reactive biotin compounds with a variety of reactive functional groups.

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Amine reactive biotin reagents, HOOK™

Supplier: G-Biosciences

HOOK™ d-Biotin (Vitamin H), 500 mg

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Annexin V conjugates

Annexin V conjugates

Supplier: Biotium

Annexin V fluorescent conjugates can be used to detect apoptotic cells. Annexin V is available conjugated to a wide variety of dyes.

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Phalloidin conjugates

Phalloidin conjugates

Supplier: Biotium

Phalloidin is a toxin isolated from the deadly Amanita phalloides mushroom. It is a bicyclic peptide that binds specifically to F-actin. Fluorescent conjugates of phalloidin are convenient tools to investigate the distribution of F-actin in fixed and permeabilised cells or tissue sections. One unit (U) of phallodin is sufficient to stain one sample (200 µl staining volume). A highly selective actin filament stain for fixed and permeabilised cells. Labeled phalloidins have similar affinity for both large and small filaments.

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CF® Dye hydrazide

CF® Dye hydrazide

Supplier: Biotium

Fluorescent CF® Dye hydrazides are bright, extremely water-soluble, nontoxic molecules, useful as aldehyde-fixable cell tracers. They can also be used to label aldehyde or ketone groups of polysaccharides, carbohydrates or glycoproteins.

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Tyramide amplification kit with HRP goat, anti-rabbit and CF® dye or biotin tyramide

Supplier: Biotium

Kits for increasing immunofluorescence sensitivity using HRP-catalysed tyramide signal amplification with the choice of next-generation fluorescent CF® dyes or biotin.

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CF™ Dyes Cholera Toxin Subunit B, CF™ Dye Conjugates

CF™ Dyes Cholera Toxin Subunit B, CF™ Dye Conjugates

Supplier: Biotium

Cholera toxin subunit B conjugated to Biotium's next-generation fluorescent CF™ dyes.

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Antikörper-Markierung-Kits, Mix-n-Stain™ Biotin

Antikörper-Markierung-Kits, Mix-n-Stain™ Biotin

Supplier: Biotium

Das Mix-n-Stain™ Biotin Antikörper-Markierungs-Kit nutzt eine Technologie, die eine schnelle Antikörpermarkierung mit Biotin ohne Aufreinigungsschritt ermöglicht.

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Stabiliser for calibrators and controls, SeramunStab® STD

Supplier: Apollo Scientific

Liquid product yellow odourless pH-value 7,3 to 7,5 Boiling point 102 °C. Stabiliser for complex mixtures of biomolecules.

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Stabiliser for sensitive biomolecules, SeramunStab® STB

Supplier: Apollo Scientific

Liquid product colourless to slightly yellow, freshly solutions opaleszent, odour weakly pH 7,3 to 7,5 boiling point 104 °C. Stabiliser for sensitive biomolecules like proteohormones or membrane proteins.

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