Protein- Expression
Protein-Expressionssysteme erleichtern erheblich die sensitive Detektion verschiedener Proteine, die häufig in der Bioanalytik und Molekularbiologie eingesetzt werden. Fluorophore oder bestimmte Vitamine werden verwendet, um Antikörper oder andere Proteine zu markieren, und diese gekennzeichneten Moleküle können dann mithilfe von anderen Lösungen wie Streptavidin oder monomeres Avidin gereinigt oder nachgewiesen werden. Diese Protein-Expressionssysteme sind optimal auf eine schnelle Synthese von funktionell aktiven Proteinen ausgerichtet.
Cell-free protein expression system ALiCE for Scale-Up
Supplier: LENIOBIO
ALiCE® - Linearly scalable eukaryotic cell-free protein expression. Scalable protein expression with ALiCE® works as effectively for assessment of future development as it does in the exploration phase. The same simple protocol applies across all scales. Add the DNA sequence and let ALiCE® make your protein.
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TriLink® CleanCap® M6 EGFP mRNA (N1MePsU)
Supplier: TriLink BioTechnologies
The EGFP mRNA expresses an enhanced version of the green fluorescent protein, originally isolated from the jellyfish, Aequorea victoria. EGFP is a commonly used direct detection reporter in mammalian cell culture, yielding bright green fluorescence with an emission peak at 509 nm.
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Alum adjuvant, AlumVax hydroxide
Supplier: OZ BIOSCIENCES
AlumVax hydroxide is a wet gel (colloidal) of aluminium hydroxide 2%. It is the most common adjuvant used in approved prophylactic vaccines because of its excellent safety profile and ability to enhance the protective humoral immune response.
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Freund's incomplete adjuvant, Imject™
Supplier: Thermo Fisher Scientific
Imject™ Freund's Incomplete Adjuvant (FIA) is a popular non specific stimulator of the immune response for use in antibody production immunisation protocols.
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ProLite™ His-Tag Protein Gel Staining Kit
Supplier: AAT Bioquest
This kit requires very little hands-on time, allowing for rapid protein expression screening in a variety of gel types using an imaging equipment with a standard FITC filter.
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Squalene emulsion, SqualVax
Supplier: OZ BIOSCIENCES
SqualVax is an oil-in-water emulsion made of squalene droplets in a continuous aqueous phase. It is an important advantage over alternative oils that have been used in emulsion adjuvants, like Freund’s adjuvant that contains mineral oil (paraffin oil) and has long term persistence in organisms.
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HA(FL)-H1N1 mRNA
Supplier: OZ BIOSCIENCES
HA(FL)-H1N1 mRNA has been designed to produce high expression level of the full length (HA) hemagglutinin surface glycoproteins.
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Freund's incomplete adjuvant, IFAVax
Supplier: OZ BIOSCIENCES
Incomplete Freund’s Adjuvant (IFA) is a water-in-oil emulsion that is similar to the complete Freund’s Adjuvant (CFA) without addition of heat-killed mycobacteria (Mycobacterum butyricum).
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Cell-free protein expression kits, 1-step human coupled IVT kit - DNA
Supplier: Thermo Fisher Scientific
The 1-Step Human Coupled IVT Kit – DNA is a mammalian in vitro translation (IVT) system based on HeLa cell lysates. The kit contains all of the cellular components required for protein synthesis, including ribosomes, initiation factors, elongation factors and tRNA. When supplemented with the included proprietary accessory proteins, reaction mix and a DNA template cloned into the Thermo Scientific pT7CFE1-based vector, this system can synthesise protein for up to 6 hours. The kit includes a pT7CFE-CHis vector for ease of use in downstream applications.
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Cell-free protein expression kits, 1-step human high yield mini IVT kit
Supplier: Thermo Fisher Scientific
The Thermo Scientific 1-Step Human High-Yield In Vitro Translation (IVT) Kits enable the expression of functional proteins with 10 to 100 times greater yield per mL than other mammalian IVT systems.
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Pierce™ Cell-free Protein Expression Kits, 1-Step Human High Yield Maxi IVT Kit
Supplier: Thermo Fisher Scientific
The 1-Step Human High-Yield In Vitro Translation (IVT) Kits enable the expression of functional proteins with 10 to 100 times greater yield per ml than other mammalian IVT systems.
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Autoinduction systems, Overnight Express™ Instant LB and TB Media
Supplier: Merck Millipore (Novagen)
The Overnight Express™ Autoinduction Systems enable regulated protein expression in E. coli, without monitoring the culture or adding inducer during cell growth.
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Freund's complete adjuvant, Imject™
Supplier: Thermo Fisher Scientific
Imject™ Freund's Complete Adjuvant (FCA) is a popular non specific stimulator of the immune response for use in antibody production immunisation protocols.
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Freund's complete adjuvant, CFAVax
Supplier: OZ BIOSCIENCES
Complete Freund’s Adjuvant (CFA) is a water-in-oil emulsion containing 1 mg per ml heat-killed Mycobacterium tuberculosis.
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Alum adjuvant, AlumVax phosphate
Supplier: OZ BIOSCIENCES
AlumVax phosphate is a wet gel (colloidal) of aluminium phosphate 2%. Aluminium salts are the most common adjuvants used in approved prophylactic vaccines because of their excellent safety profile and ability to enhance the protective humoral immune response.
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Autoinduction systems, Overnight Express™ NMR Media
Supplier: Merck Millipore (Novagen)
The Overnight Express™ Autoinduction Systems enable regulated protein expression in E. coli, without monitoring the culture or adding inducer during cell growth.
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