Protein- Expression

Supplier: TriLink BioTechnologies

Ovalbumin (OVA) is a member of the serpin superfamily and the predominant glycoprotein found in egg whites. It is a commonly used antigen for immunisation and biochemical studies and is an established model allergen for airway hyper-responsiveness.

Supplier: TriLink BioTechnologies

NLS-Cre Recombinase mRNA is a capped and polyadenylated messenger RNA encoding Cre recombinase fused to a nuclear localisation sequence (NLS). Cre recombinase is a tyrosine recombinase that catalyses recombination between two loxP sites.

Supplier: TriLink BioTechnologies

This construct expresses a firefly luciferase (FLuc) protein, originally isolated from the firefly Photinus pyralis, as self-amplifying RNA (saRNA). FLuc is commonly used in mammalian cell culture to measure both gene expression and cell viability. It emits bioluminescence in the presence of the substrate, luciferin.

Supplier: TriLink BioTechnologies

mCherry mRNA encodes the fluorescent protein, mCherry, which is derived from DsRed, a protein found in Discosoma sp. mCherry is a monomeric fluorophore with a peak absorption at 587 nm and emission at 610 nm. It is stable and resistant to photobleaching.

Supplier: TriLink BioTechnologies

The FLuc mRNA expresses a luciferase protein, originally isolated from the firefly, Photinus pyralis. FLuc is commonly used in mammalian cell culture to measure both gene expression and cell viability. It emits bioluminescence in the presence of the substrate, luciferin.

Supplier: TriLink BioTechnologies

EPO mRNA encodes the human erythropoietin (EPO) protein, a hormone that controls erythropoiesis, or red blood cell production. EPO also plays a role in wound healing and the brain’s response to neural injury. Kariko et al. showed that transfection of EPO mRNA in vivo resulted in significant increases of both reticulocyte counts and hematocrits.

Supplier: LENIOBIO

From ALiCE® for Research to ALiCE® for Scale-Up Protein Expression, we offer cell-free protein expression products with radical results for every production setup.

Supplier: TriLink BioTechnologies

β-galactosidase (β-gal), a protein product of the bacterial LacZ gene, catalyzes the conversion of β-galactosides into monosaccharides. β-gal is a common marker gene used to assess transfection efficiency.

Supplier: TriLink BioTechnologies

Cas9 Nickase mRNA expresses a version of the Streptococcus pyogenes SF370 Cas9 protein (CRISPR Associated Protein 9) that contains a D10A amino acid substitution. This mRNA also contains a C-terminal nuclear localisation signal followed by a HA tag.

Supplier: TriLink BioTechnologies

Ovalbumin (OVA) is a member of the serpin superfamily and the predominant glycoprotein found in egg whites. It is a commonly used antigen for immunisation and biochemical studies and is an established model allergen for airway hyper-responsiveness.

Supplier: TriLink BioTechnologies

Cas9 mRNA expresses a version of the Streptococcus pyogenes SF370 Cas9 protein (CRISPR Associated Protein 9). Cas9 functions as part of the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) genome editing system. In the CRISPR system, an RNA guide sequence targets the site of interest and the Cas9 protein is employed to perform double stranded DNA cleavage.

Supplier: TriLink BioTechnologies

Dieses mRNA-Konstrukt, hergestellt über in-vitro- Transkription (IVT), exprimiert ein Luciferase-Protein, das ursprünglich aus dem Glühwürmchen isoliert wurde, Photinus pyralis. Glühwürmchen-Lucifrase (FLuc) wird häufig in der Säugetierzellkultur verwendet, um sowohl die Genexpression als auch die Zelllebensfähigkeit zu messen. In Gegenwart des Substrats Luciferin emittiert es Biolumineszenz.

Supplier: TriLink BioTechnologies

Dieses mRNA-Konstrukt, hergestellt über in-vitro- Transkription (IVT), drückt eine Version der Streptococcus pyogenes SF370 CRISPR Associated Protein 9 (Cas9) Protein. Cas9 fungiert als Teil des CRISPR-Genomeditierungssystems (Clustered Regularly Interspaced Short Palindromic Repeats). Im CRISPR-System wird eine RNA-Leitsequenz auf die gewünschte Stelle gerichtet, und das Cas9-Protein wird zur Spaltung doppelsträngiger DNA eingesetzt.

Supplier: TriLink BioTechnologies

TriLink’s CleanCap® AG (3′ OMe) CleanScript™ IVT Kit provides essential components for mRNA synthesis by in vitro transcription (IVT) with co-transcriptional capping. The kit components are sufficient for 25×100 µl or 125×20 µl reactions following our recommended IVT protocol. It includes a cap analog, modified and unmodified nucleotides, IVT enzymes, a reaction buffer, and a control DNA template. Each kit is expected to yield 20-25 mg of capped mRNA.

Supplier: TriLink BioTechnologies

The EGFP mRNA expresses an enhanced version of the green fluorescent protein, originally isolated from the jellyfish, Aequorea victoria. EGFP is a commonly used direct detection reporter in mammalian cell culture, yielding bright green fluorescence with an emission peak at 509 nm.

Supplier: TriLink BioTechnologies

Renilla luciferase (Renilla Luc) is a bioluminescent reporter protein derived from Renilla reniformis (sea pansy). In the presence of the cofactor, coelenterazine, Renilla luciferase produces light. Renilla Luc mRNA can be used as an internal control in combination with FLuc mRNA to test different conditions, such as dosage or chemical modifications, and their effect on translation.