Die gegen pH-Änderungen beständigen Puffer schützen Proben bei Zugabe von Säuren oder Basen. In Form von Pulvern oder Flüssigkeiten werden sie effizient zur elekrophoretischen Isolierung und Trennung von Proteinen eingesetzt. Die salzhaltigen Puffer können auch zur Kalibrierung und Standardisierung von pH-Messgeräten verwendet werden, um genaue Messergebnisse zu gewährleisten. Blocking-Puffer verhindern die Anbindung von Antikörpern an freie Membran-Bindungsstellen, die ansonsten einen störenden Hintergrund verursachen würden. Die Puffer sind je nach Bedarf in verschiedenen Konzentrationen sowie in flüssiger oder getrockneter Form erhältlich.
MMP-1 colorimetric assay buffer
Supplier: ENZO LIFE SCIENCES
MMP-1 colorimetric assay buffer
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Buffers, sodium phosphate buffer solutions (0,5 mol/L)
Supplier: Cayman Chemical
Sodium phosphate stock solution (0,5 mol/L), pH-Wert: 8,0
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Buffered NaCl Peptone Solution pH 7,0
Supplier: LIOFILCHEM
Buffered NaCl Peptone Solution pH 7,0 is used for dissolving, suspending and diluting test samples. Its composition conforms to recommendations in the Harmonised Pharmacopoeias for the microbiological examination of non-sterile products.
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Western-Blot-Transferpuffer, 10X, methanolfrei, Pierce™
Supplier: Thermo Fisher Scientific
Pierce™ 10X Western-Blot-Transferpuffer ohne Methanol ist eine platzsparende Stammlösung zur Zubereitung eines methanolfreien Transferpuffers für gekühlte, Tank- (nasse) Western-Blotting-Transfersysteme.
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J.T.Baker®, Sheath fluid, 3200/3400
Supplier: Avantor
Sheath fluid, 3200/4000, J.T.Baker®
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Blocking buffers, Protein-Free™
Supplier: G-Biosciences
Protein-Free™ Blocking buffer is a formulation of non-protein agents that eliminate non-specific binding sites in ELISA, blotting, immunohistochemistry and other applications. The absence of protein from the Protein-Free™ Blocking buffer eliminates problems associated with traditional protein based blockers, such as cross-reactivity and interference from glycosylated proteins.
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HEPES Pufferlösung pH 7,3 (1 mol/l)
Supplier: VWR Chemicals
HEPES Pufferlösung pH 7,3 (1 mol/l), VWR®
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TRIS Pufferlösung pH 7,5 (1 mol/l), ultrarein
Supplier: VWR Chemicals
TRIS Pufferlösung pH 7,5 (1 mol/l), VWR®
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SSC-Puffer-Lösung 20fach konzentriert (citratgepufferte Salzösung), Corning®
Supplier: Corning
20X Saline-Sodium Citrate (SSC) buffer is used in both nucleic acid hybridization and blot transfer procedures
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Dulbecco's phosphate buffered saline (DPBS), for cell culture, powder format, HyClone™
Supplier: HyClone products (Cytiva)
Balanced salt solution used for multiple cell culture applications (powder form).
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ES Buffer
Supplier: FUJIFILM WAKO CHEMICALS
The ES Buffer is an endotoxin-specific buffer that is ideal for untreated LAL.
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HDAC assay buffer II
Supplier: ENZO LIFE SCIENCES
50 mM Tris/Cl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, 1 mg/ml BSA.
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Protein blocking agent, BLOT-QuickBlocker™
Supplier: G-Biosciences
BLOT-QuickBlocker™ is a modified milk protein that is highly soluble and does not inhibit peroxidase detection. The modified milk protein has high blocking efficiency with a clear background.
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WB blocking buffer kit, TrueBlack®
Supplier: Biotium
The TrueBlack® WB blocking buffer kit is a ready-to-use buffer system that provides optimal specificity, sensitivity, and background signal suppression for fluorescence-based western blotting (WB).
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Dry buffer packs, Carbonate-Bicarbonate Buffer, JAW™
Supplier: G-Biosciences
Premixed buffer powders that simply require adding deionised water to generate a ready-to-use buffer.
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Tween-20 solution (10%)
Supplier: ENZO LIFE SCIENCES
This Tween®-20 solution (10%) is specially designed to create the Blocking Buffer and Assay Buffer in ImmunoSet products. Each bottle is sufficient for use with 5×96 wells microtiter plates if following the instructions in our ImmunoSet protocol.
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IHC PBS tablets
Supplier: ENZO LIFE SCIENCES
Solid. 100 ml PBS (1X) tablets containing 137 mM sodium chloride, 2.7 mM potassium chloride, and 10 mM phosphate buffer.
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Neutralising reagent
Supplier: ENZO LIFE SCIENCES
Neutralising buffer for low pH solutions and for use with cyclic nucleotide and isoprostane kits.
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Background suppressor system (Permeabilising), TrueBlack® IF
Supplier: Biotium
A buffer set that blocks non-specific antibody binding as well as direct interaction of fluorescent dyes on antibodies with cells or tissue sections.
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Transfer or Electro Blotting buffer (10X), pH 8,5
Supplier: Thermo Fisher Scientific
Elektrophorese-Puffer, Transfer buffer, Transfer Electroblot Buffer 10X
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Blocking buffers, FirstChoice™
Supplier: G-Biosciences
FirstChoice™ Blocking Buffer is ideal as a first choice for optimization of new assays and systems, or when determining the optimal blocking buffer for elimination of non specific binding sites in ELISA, blotting, immunohistochemistry and other applications. Buffers are conveniently supplied in widely used TBS (Tris-buffered saline at pH 7,5) and PBS (phosphate-buffered saline at pH 7,5) buffers as well as in separate formulations containing Tween® 20 for improving blocking efficiencies.
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Optimizer Buffer™ for optimal cross-linking and modification
Supplier: G-Biosciences
The conjugation and modification reactions used to cross-link proteins or couple labels to proteins, such as biotin, enzymes, and fluorescent dyes, require certain conditions, including pH and chemical composition for optimal conjugation. Many common buffers routinely used in laboratories have an inhibitory effect on conjugation reactions; for example, Tris buffers inhibit coupling to amines.