Die gegen pH-Änderungen beständigen Puffer schützen Proben bei Zugabe von Säuren oder Basen. In Form von Pulvern oder Flüssigkeiten werden sie effizient zur elekrophoretischen Isolierung und Trennung von Proteinen eingesetzt. Die salzhaltigen Puffer können auch zur Kalibrierung und Standardisierung von pH-Messgeräten verwendet werden, um genaue Messergebnisse zu gewährleisten. Blocking-Puffer verhindern die Anbindung von Antikörpern an freie Membran-Bindungsstellen, die ansonsten einen störenden Hintergrund verursachen würden. Die Puffer sind je nach Bedarf in verschiedenen Konzentrationen sowie in flüssiger oder getrockneter Form erhältlich.
Dulbecco's phosphate buffered saline (DPBS), cell culture, MP Biomedicals
Supplier: MP Biomedicals
Phosphate buffered salts, Dulbecco's formula (DPBS) is a balanced salt solution (BSS) used for the handling and culturing of mammalian cells. DPBS is used to to irrigate, wash, and dilute mammalian cells.
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Hank's balanced salts, MP Biomedicals
Supplier: MP Biomedicals
Hanks' balanced salts solution is used to maintain the pH and osmotic balance in the medium and to provide the cells with water and essential inorganic ions.
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SSC 20X buffer, MP Biomedicals
Supplier: MP Biomedicals
Role of a balanced salt solution in cell culture is multifaceted and can be divided into four principal functions:
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2-D Protein Extraction Buffer
Supplier: Cytiva
Efficient cell lysis and protein extraction are key steps for achieving high-quality results in downstream applications. The 2-D protein extraction buffer trial kit is designed for those who experiment for establishing a suitable extraction and solubilization buffer for their protein samples.
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EDTA disodium salt solution, 0,2% in phosphate buffered saline, MP Biomedicals
Supplier: MP Biomedicals
EDTA disodium salt, (2%) solution, in phosphate buffered saline without calcium and magnesium. Used as a gentle non-enzymatic cell dissociation reagent.
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Ab buffer kit
Supplier: Cytiva
Ab buffer kit contains prepared buffer concentrates for binding, washing, and elution of IgG according to recommended protocols.
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DIGE buffer kit
Supplier: Cytiva
DIGE buffer kit is a tool to increase the quality, reproducibility, and convenience of 2-D DIGE using the Ettan™ DIGE system. The DIGE buffer kit is a specially designed two-buffer system for running up to twelve DIGE gels simultaneously.
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Saline solutions
Supplier: BALIS
Cell culture buffer, Physiological saline solution, 9 ml, no standards
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TISAB III-Lösung (Gesamtionenstärkenpuffer), Konzentrat für die Fluoridbestimmung
Supplier: REAGECON
TISAB III-Lösung (Gesamtionenstärkenpuffer), Konzentrat
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Waschlösungskonzentrat, 20X
Supplier: SERACARE LIFE SCIENCES
Praktisches 20-faches-Konzentrat mit Imidazol-gepufferter Kochsalzlösung und Tween® 20
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Buffer, EDTA buffer solution pH 8,0 (0,5 mol/l)
Supplier: EUROCLONE
Cloning Buffer, EDTA buffer solution pH 8,0 (0,5 mol/l)
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Buffer, EDTA buffer solution pH 8,0 (0,25 mol/L)
Supplier: EUROCLONE
Cloning Buffer, EDTA buffer solution pH 8,0 (0,25 mol/l)
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TRIS Pufferlösung pH 8 (2 mol/l)
Supplier: EUROCLONE
Cloning Buffer, TRIS Pufferlösung pH 8 (2 mol/l)
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TRIS Pufferlösung pH 7,5 (2 mol/l)
Supplier: EUROCLONE
Cloning Buffer, TRIS Pufferlösung pH 7,5 (2 mol/l)
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Glucose hemolysing solution
Supplier: DIAGNOSTIC SYSTEMS DIASYS
For use with glucose Gluc-DH FS and glucose Gluc-DH FSC.
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Ambion® buffer, potassium acetate, 3M™ solution, pH 5,5, RNase-free, Invitrogen™
Supplier: Ambion
Cloning Buffer, Potassium acetate buffer solution pH 5,5 (3 mol/l)
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Ambion® Buffer, EDTA buffer solution pH 8,0 (0,5 mol/l), Invitrogen™
Supplier: Ambion
Cloning Buffer, EDTA buffer solution pH 8,0 (0,5 mol/l)
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Ambion® buffer, sodium acetate buffer solution pH 5,5 (3 mol/L), RNase-free, Invitrogen™
Supplier: Ambion
Cloning Buffer, Sodium acetate buffer solution pH 5,5 (3 mol/l)
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Phosphate Buffered Saline
Supplier: THERMO FISHER DIAGNOSTICS
Cell culture buffer, Phosphate buffered saline tablets