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Puffer

Die gegen pH-Änderungen beständigen Puffer schützen Proben bei Zugabe von Säuren oder Basen. In Form von Pulvern oder Flüssigkeiten werden sie effizient zur elekrophoretischen Isolierung und Trennung von Proteinen eingesetzt. Die salzhaltigen Puffer können auch zur Kalibrierung und Standardisierung von pH-Messgeräten verwendet werden, um genaue Messergebnisse zu gewährleisten. Blocking-Puffer verhindern die Anbindung von Antikörpern an freie Membran-Bindungsstellen, die ansonsten einen störenden Hintergrund verursachen würden. Die Puffer sind je nach Bedarf in verschiedenen Konzentrationen sowie in flüssiger oder getrockneter Form erhältlich.

Dulbecco's phosphate buffered saline (DPBS), cell culture, MP Biomedicals

Dulbecco's phosphate buffered saline (DPBS), cell culture, MP Biomedicals

Supplier: MP Biomedicals

Phosphate buffered salts, Dulbecco's formula (DPBS) is a balanced salt solution (BSS) used for the handling and culturing of mammalian cells. DPBS is used to to irrigate, wash, and dilute mammalian cells.

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Hank's balanced salts, MP Biomedicals

Supplier: MP Biomedicals

Hanks' balanced salts solution is used to maintain the pH and osmotic balance in the medium and to provide the cells with water and essential inorganic ions.

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SSC 20X buffer, MP Biomedicals

Supplier: MP Biomedicals

Role of a balanced salt solution in cell culture is multifaceted and can be divided into four principal functions:

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2-D Protein Extraction Buffer

2-D Protein Extraction Buffer

Supplier: Cytiva

Efficient cell lysis and protein extraction are key steps for achieving high-quality results in downstream applications. The 2-D protein extraction buffer trial kit is designed for those who experiment for establishing a suitable extraction and solubilization buffer for their protein samples.

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EDTA disodium salt solution, 0,2% in phosphate buffered saline, MP Biomedicals

Supplier: MP Biomedicals

EDTA disodium salt, (2%) solution, in phosphate buffered saline without calcium and magnesium. Used as a gentle non-enzymatic cell dissociation reagent.

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Ab buffer kit

Ab buffer kit

Supplier: Cytiva

Ab buffer kit contains prepared buffer concentrates for binding, washing, and elution of IgG according to recommended protocols.

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DIGE buffer kit

DIGE buffer kit

Supplier: Cytiva

DIGE buffer kit is a tool to increase the quality, reproducibility, and convenience of 2-D DIGE using the Ettan™ DIGE system. The DIGE buffer kit is a specially designed two-buffer system for running up to twelve DIGE gels simultaneously.

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Saline solutions

Supplier: BALIS

Cell culture buffer, Physiological saline solution, 9 ml, no standards

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TISAB III-Lösung (Gesamtionenstärkenpuffer), Konzentrat für die Fluoridbestimmung

Supplier: REAGECON

TISAB III-Lösung (Gesamtionenstärkenpuffer), Konzentrat

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HEPES Pufferlösung (1 mol/l)

Supplier: EUROCLONE

HEPES Pufferlösung (1 mol/l)

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HEPES Pufferlösung (1 mol/l)

Supplier: EUROCLONE

HEPES Pufferlösung (1 mol/l)

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Waschlösungskonzentrat, 20X

Supplier: SERACARE LIFE SCIENCES

Praktisches 20-faches-Konzentrat mit Imidazol-gepufferter Kochsalzlösung und Tween® 20

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Buffer, EDTA buffer solution pH 8,0 (0,5 mol/l)

Supplier: EUROCLONE

Cloning Buffer, EDTA buffer solution pH 8,0 (0,5 mol/l)

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Buffer, EDTA buffer solution pH 8,0 (0,25 mol/L)

Supplier: EUROCLONE

Cloning Buffer, EDTA buffer solution pH 8,0 (0,25 mol/l)

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TRIS Pufferlösung pH 8 (2 mol/l)

Supplier: EUROCLONE

Cloning Buffer, TRIS Pufferlösung pH 8 (2 mol/l)

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TRIS Pufferlösung pH 7,5 (2 mol/l)

Supplier: EUROCLONE

Cloning Buffer, TRIS Pufferlösung pH 7,5 (2 mol/l)

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Glucose hemolysing solution

Glucose hemolysing solution

Supplier: DIAGNOSTIC SYSTEMS DIASYS

For use with glucose Gluc-DH FS and glucose Gluc-DH FSC.

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Ambion® buffer, potassium acetate, 3M™ solution, pH 5,5, RNase-free, Invitrogen™

Supplier: Ambion

Cloning Buffer, Potassium acetate buffer solution pH 5,5 (3 mol/l)

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Ambion® Buffer, EDTA buffer solution pH 8,0 (0,5 mol/l), Invitrogen™

Supplier: Ambion

Cloning Buffer, EDTA buffer solution pH 8,0 (0,5 mol/l)

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Ambion® buffer, sodium acetate buffer solution pH 5,5 (3 mol/L), RNase-free, Invitrogen™

Supplier: Ambion

Cloning Buffer, Sodium acetate buffer solution pH 5,5 (3 mol/l)

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Phosphate Buffered Saline

Supplier: THERMO FISHER DIAGNOSTICS

Cell culture buffer, Phosphate buffered saline tablets

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HEPES Pufferlösung (1 mol/l)

Supplier: Invitrogen

HEPES Pufferlösung (1 mol/l)

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