Sie haben gesucht nach: Puffer
Die gegen pH-Änderungen beständigen Puffer schützen Proben bei Zugabe von Säuren oder Basen. In Form von Pulvern oder Flüssigkeiten werden sie effizient zur elekrophoretischen Isolierung und Trennung von Proteinen eingesetzt. Die salzhaltigen Puffer können auch zur Kalibrierung und Standardisierung von pH-Messgeräten verwendet werden, um genaue Messergebnisse zu gewährleisten. Blocking-Puffer verhindern die Anbindung von Antikörpern an freie Membran-Bindungsstellen, die ansonsten einen störenden Hintergrund verursachen würden. Die Puffer sind je nach Bedarf in verschiedenen Konzentrationen sowie in flüssiger oder getrockneter Form erhältlich.
Buffer, SSC 20X powder for molecular biology, MP Biomedicals
Supplier: MP Biomedicals
SSC buffer used in electrophoresis and in sedimentation studies of DNA-histone aggregates. SSC is used as a hybridisation buffer in immunoblotting and DNA microarray protocols.
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DNA gel loading buffer 6X
Supplier: LONZA
This buffer is a Ficoll based loading buffer containing Ficoll®, EDTA, xylene cyanol, and bromophenol blue. Ideal for separation of DNA fragments with improved resolution.
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PBS, 10X solution, molecular biology grade, ultrapure
Supplier: Thermo Fisher Scientific
Waschpuffer, PBS, 10X solution, molecular biology grade, ultrapure
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TRIS-buffered saline (TBS, 20X), with 0,5% Tween 20
Supplier: Thermo Fisher Scientific
Waschpuffer, TRIS-buffered saline (TBS, 20X), with 0,5% Tween 20
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His·Bind® buffer kit, Novagen®
Supplier: Merck Millipore (Novagen)
The His·Bind buffer kit is a set of pre-tested buffers designed for use with IDA-based His·Bind resin for convenient, rapid one-step purification of proteins by metal chelation chromatography.
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Protein A/G HP SpinTrap™ Buffer Kit
Supplier: Cytiva
Protein A/G HP SpinTrap™ Buffer kit is optimised for standard affinity-based enrichment/immunoprecipitation according to the protocol provided with protein A HP SpinTrap™, protein G HP SpinTrap™, and Ab SpinTrap™ columns.
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His buffer kit
Supplier: Cytiva
His Buffer Kit contains carefully prepared buffer concentrates for binding, washing, and elution of histidine-tagged proteins according to recommended protocols. Moreover, a method for optimization of imidazole concentration in the binding buffer is described.
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Transformation buffer-2, pH 7,5
Supplier: Thermo Fisher Scientific
Cloning Buffer, Transformation buffer
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Cell lysis buffers
Supplier: Rockland Immunochemicals
Cell lysis buffer is used to lyse cells under non-denaturing conditions.
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Enzyme storage buffer in PBS and glycerol, autoclaved
Supplier: Thermo Fisher Scientific
Enzyme Storage Buffer in PBS and Glycerol
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Tris-HCl, 1M solution, pH 8,0, Molecular Biology Grade, Ultrapure
Supplier: Thermo Fisher Scientific
1M solution of Tris, Ultrapure in high purity distilled H₂O, adjusted to pH 8,0.
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Ionisation buffer solutions (CRMs), for AAS and ICP, Fluka™
Supplier: Honeywell Chemicals
Matrix Modifier, 2% Cesium (Cs) in 1% HNO₃
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Lysis buffer, 0,5% Tween 20, pH 8,0, for isolation of His-tag modified proteins
Supplier: Thermo Fisher Scientific
Lysis buffer, 0.5% Tween 20, pH 8.0, for isolation of His-tag modified proteins, Thermo Fisher Scientific
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Protexidase™, peroxidase protection buffer, Immuno™, MP Biomedicals
Supplier: MP Biomedicals
A peroxidase protective buffer which stabilises HRP conjugates. It has 500 ml of PBS, pH 7,2, containing 0,5% BSA, antimicrobial preservatives and proprietary stabilising compounds.
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EDTA solution, 0,5 M, molecular biology grade, Calbiochem®, Millipore®
Supplier: Merck Millipore (Calbiochem)
Sterile-filtered solution of 0,5 M EDTA in H₂O treated with diethyl pyrocarbonate (DEPC). Used in molecular biology applications.
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Buffer, sodium acetate buffer solution pH 5,2 (3 mol/l), Millipore®
Supplier: Merck Millipore (Calbiochem)
Cloning Buffer, Sodium acetate buffer solution pH 5,2 (3 mol/l)
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NP-40 lysis buffer
Supplier: Thermo Fisher Scientific
NP-40 lysis buffer, Thermo Fisher Scientific
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Triton® X-100 lysis buffer, pH 7,4
Supplier: Thermo Fisher Scientific
Triton® X-100 lysis buffer, pH 7.4, Thermo Fisher Scientific
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TRIS Pufferlösung pH 7,4 (1 mol/l)
Supplier: Thermo Fisher Scientific
Cloning Buffer, TRIS Pufferlösung pH 7,4 (1 mol/l)
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NP-40 lysis buffer with glycerol (2X)
Supplier: Thermo Fisher Scientific
NP-40 lysis buffer with glycerol (2X), Thermo Fisher Scientific
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Dulbecco's phosphate buffered saline (DPBS), cell culture, MP Biomedicals
Supplier: MP Biomedicals
Phosphate buffered salts, Dulbecco's formula (DPBS) is a balanced salt solution (BSS) used for the handling and culturing of mammalian cells. DPBS is used to to irrigate, wash, and dilute mammalian cells.
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Hank's balanced salts, MP Biomedicals
Supplier: MP Biomedicals
Hanks' balanced salts solution is used to maintain the pH and osmotic balance in the medium and to provide the cells with water and essential inorganic ions.
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Buffer, EDTA buffer solution pH 8,0 (0,5 mol/l), Millipore®
Supplier: Merck Millipore (Calbiochem)
Cloning Buffer, EDTA buffer solution pH 8,0 (0,5 mol/l)
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Protein extraction reagent, BugBuster® 10X, Millipore®
Supplier: Merck Millipore (Novagen)
BugBuster® 10X is a concentrated formulation of the proprietary detergents employed in BugBuster® without the addition of salts or buffer components. Concentrated BugBuster® provides a flexible alternative to the ready-to-use standard 1X BugBuster®, allowing user-defined dilution and addition of buffer components. BugBuster® 10X has all of the bioprocessing benefits of standard BugBuster® plus the freedom to control pH, reagent concentration, and buffer additives necessary for maximum extraction and activity of your target protein.
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RIPA Lysis and Extraction Buffer
Supplier: G-Biosciences
A complete lysis buffer for the release of cytoplasmic, membrane and nuclear proteins from adherent and suspension cultured mammalian cells. The RIPA lysis buffer is fully compatible with many applications, including reporter assays, protein assays, immunoassays and other protein purification techniques.
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Factor XA protease digestion buffer
Supplier: Thermo Fisher Scientific
Factor XA Protease Digestion Buffer
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TRIS Pufferlösung pH 8 (1 mol/l)
Supplier: Thermo Fisher Scientific
Cloning Buffer, TRIS Pufferlösung pH 8 (1 mol/l)
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Tris-Glycine (TG) buffer,, MP Biomedicals
Supplier: MP Biomedicals
Tris-glycine buffer is used for western blotting and gel electrophoresis.
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HEPES Pufferlösung (1 mol/l)
Supplier: MP Biomedicals
HEPES (N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid) is a general purpose zwitterionic organic chemical buffering agent which does not bind magnesium, calcium, manganese(II) or copper (II) ions.
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Hank's balanced salt solution, modified, MP Biomedicals
Supplier: MP Biomedicals
Cell culture buffer, Hank's balanced salt solution, modified (1X)