Sie haben gesucht nach: Tests

Supplier: Aviva Systems Biology

Das Trap1a ELISA-Kit (Maus) basiert auf der Standard-Sandwich-Enzyme-Linked-Immuno-Sorbent-Assay-Technologie. Ein für Trap1a spezifischer Antikörper wurde auf eine 96-Well-Platte (12×8-Well-Streifen) Block vorbeschichtet. Standards oder Testproben werden in die Vertiefungen gegeben, inkubiert und entnommen. Ein für Trap1a spezifischer biotinylierter Detektorantikörper wird hinzugefügt, inkubiert und anschließend gewaschen. Anschließend wird das Avidin-Peroxidase-Konjugat zugegeben, inkubiert und nicht gebundenes Konjugat abgewaschen. Durch die Zugabe von TMB-Substrat wird eine enzymatische Reaktion erzeugt, die durch HRP katalysiert wird und ein blaues Farbprodukt erzeugt, das sich nach Zugabe einer sauren Stopplösung in Gelb ändert. Die anhand der Absorption bei 450 nm abgelesene Dichte der Gelbfärbung ist quantitativ proportional zur Menge der in der Well eingefangenen Probe Trap1a.

Supplier: Aviva Systems Biology

Das B2m ELISA-Kit (Maus) (OKEH00344) von Aviva Systems Biology basiert auf der standardmäßigen Sandwich-Enzym-Linked-Immunosorbent-Assay-Technologie. Ein für B2m spezifischer Antikörper wurde auf eine 96-Well-Platte (12×8-Well-Streifen) vorbeschichtet. Standards oder Testproben werden in die Vertiefungen gegeben, inkubiert und entnommen. Ein für B2m spezifischer biotinylierter Detektorantikörper wird hinzugefügt, inkubiert und anschließend gewaschen. Anschließend wird das Avidin-Peroxidase-Konjugat zugegeben, inkubiert und nicht gebundenes Konjugat abgewaschen. Durch die Zugabe von TMB-Substrat wird eine enzymatische Reaktion erzeugt, die durch HRP katalysiert wird und ein blaues Farbprodukt erzeugt, das sich nach Zugabe einer sauren Stopplösung in Gelb ändert. Die anhand der Absorption bei 450 nm abgelesene Dichte der Gelbfärbung ist quantitativ proportional zur Menge der in der Vertiefung eingefangenen Probe B2m.

Supplier: Biosensis

The Biosensis Mouse and Rat proNGF Rapid enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of rodent full-length proNGF protein in less than 4 hours in cell culture supernatants, cell lysates and tissue homogenates only if used as directed. This ELISA Kit has also successfully been used on mouse urine samples (Ryu JC et al., 2018). Please refer to the kit protocol for specific use instructions for each substrate application.

This ELISA kit contains a recombinant mouse proNGF standard expressed in E.coli and consists of a pre-coated anti-proNGF capture antibody, a biotinylated anti-proNGF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of proNGF present in samples and protein standards. A proNGF positive control (QC sample) is provided to assure consistent assay performance. This ELISA kit detects rat proNGF due to high degree of homology (96%) with mouse proNGF based on amino acid sequence, and the ability of this kit in detecting proNGF in rat PC12 cell lysates and rat brain tissue homogenate. In the absence of a true rat proNGF standard, results may be expressed as 'mouse proNGF equivalents'. This ELISA kit shows only 20% reactivity with the human form of proNGF and is therefore not suitable to quantify human proNGF. No cross-reactivity was observed with mature mouse NGF and full-length proBDNF when tested in assay buffer.

The antibodies used in this ELISA kit bind epitopes within the pro-domain (capture) and mature domain (detection) of the protein, thus this ELISA assay does not detect the pro-domain peptide. This kit has not been tested for other applications. Sufficient amount of proNGF standard is supplied to allow for spike- and recovery experiments in order to validate this ELISA assay for other sample matrices if required.

Supplier: Biotium

Calcein-AM ist eine nicht fluoreszierende, membranpermeable Verbindung. Esteraseaktivität in den Zytoplasmen lebensfähiger Zellen wandelt Calcein-AM in die grün fluoreszierende, membranimpermeable Verbindung Calcein um, welche in lebensfähigen Zellen mit intakten Plasmamembranen eingeschlossen wird. Das Zellviabilitäts-/Zytotoxizitäts-Assay-Kit für lebende und tote Tierzellen paart das Calcein-AM mit dem Lebendfarbstoff Ethidium-Homodimer-III zur Quantifizierung von lebenden und toten Zellen.

Supplier: Biotium

NucView® 488 caspase-3 substrate is a novel cell membrane-permeable fluorogenic caspase substrate designed for detecting caspase-3 activity within live cells in real time.

Supplier: 3M Food Safety

3M™ Clean-Trace™ Surface Protein (Allergen) Test Swab ALLTEC60 is a rapid, simple, and reliable solution to monitor the presence of allergens on surfaces to effectively manage cleaning.

Supplier: 3M Food Safety

3M™ Clean-Trace™ Surface protein plus test swab MPRO50 is a simple, quick, and reliable method of checking for the removal of protein residues after cleaning and quickly identifies contamination.

Supplier: AAT BIOQUEST

Cholesterol is required to build and maintain cell membranes.

Supplier: AAT BIOQUEST

Glycerol is a precursor for the synthesis of triglycerides and phospholipids in liver and adipose tissue.

Supplier: AAT BIOQUEST

Pyruvate is an important chemical compound in intracellular metabolic pathways.

Supplier: AAT BIOQUEST

Maleimides can be directly assayed spectrophotometrically at 302 nm.

Supplier: AAT BIOQUEST

Die Matrix-Metalloproteasen (MMPs) bilden eine Familie zinkabhängiger Endopeptidasen, die innerhalb der extrazellulären Matrix funktionieren.

Supplier: AAT BIOQUEST

L-alanine (L-Ala) plays a crucial role as a building block of important proteins.

Supplier: 3M Food Safety

Kit contains 96 tests and is used with the 3M™ Molecular Detection System to check for inhibition of the matrix sample during validation periods, when adopting the 3M™ method or testing new or unknown matrices.

Supplier: AAT BIOQUEST

Designed for consistent and dependable results. Amplite® Fluorimetric Biotin Quantitation Kit uses Biotinylite™ Green, a fluorogenic biotin sensor.

Supplier: AAT BIOQUEST

Glucose-6-phosphate (G6P) is a key intermediate for glucose transport into cells.

Supplier: AAT BIOQUEST

Designed for consistent and dependable results. The Amplite® Fluorimetric Lipase Activity Assay Kit provides a simple and quick protocol for measuring lipase activity.

Supplier: AAT BIOQUEST

Lactate dehydrogenase (LDH) is an oxidoreductase enzyme that catalyses the interconversion of pyruvate and lactate.

Supplier: AAT BIOQUEST

Nicotinamide adenine dinucleotide (NAD+) and nicotinamide adenine dinucleotide phosphate (NADP+) are two important cofactors found in cells.

Supplier: AAT BIOQUEST

Designed for consistent and dependable results. The Amplite® Colorimetric Hexokinase Assay Kit is designed to accurately measure the activity of hexokinases from various sources using a simple and quick protocol.

Supplier: AAT BIOQUEST

Designed for consistent and dependable results. The Amplite® Fluorimetric Asparaginase Activity Assay Kit provides a simple and straightforward procedure for measuring Asparaginase activity in a variety of biological samples.

Supplier: AAT BIOQUEST

Formaldehyde is a naturally occurring substance.

Supplier: AAT BIOQUEST

Ammonia is an important source of nitrogen for living systems.

Supplier: AAT BIOQUEST

Melanins have very diverse roles and functions in various organisms.

Supplier: AAT BIOQUEST

Hydrogen peroxide (H2O2) is a reactive oxygen metabolic by-product that serves as a key regulator for a number of oxidative stress-related states.

Supplier: AAT BIOQUEST

Urea is the final degradation product of protein and amino acid metabolism in animals.

Supplier: AAT BIOQUEST

E. coli Beta-Galactosidase ist ein 464 Kd-Tetramer.

Supplier: AAT BIOQUEST

Protein thiols are very important to protein structure, protein function and biological system redox environment.

Supplier: AAT BIOQUEST

The avidin/streptavidin-biotin interaction is the strongest known non-covalent biological interaction (Kd = 10 to 15 M⁻¹) between a protein and its ligand.

Supplier: 3M Food Safety

3M™ Clean-Trace™ Surface Protein Plus Test Swab PRO100 is a simple, quick, and reliable method of checking for the removal of protein residues after cleaning and quickly identifies contamination.