RT-PCR- und cDNA-Reagenzien

Supplier: Enzo Life Sciences

AMPIGENE® cDNA Synthesis Kit for efficient and sensitive cDNA synthesis, for downstream qPCR.

Supplier: AGILENT

AccuScript High-Fidelity Reverse Transcriptase delivers the highest RT accuracy currently available.

Supplier: Quantabio

qScript™ cDNA Synthese-Sets enthalten qScript™ Reverse Transkriptase, eine Mischung aus MMLV-Reversen-Transkriptase und einem Ribonuklease-Inhibitorprotein, optimiert für empfindliche und zuverlässige cDNA-Synthese über einen breiten dynamischen Bereich ursprünglicher RNA. cDNA-Synthesereagenzien sind sowohl als SuperMix als auch als Set für spezifische Anwendungen erhältlich.

Supplier: Quantabio

Das qScript™ XLT One-Step-RT-PCR-Kit ist ein praktisches und hochempfindliches System für die Verstärkung von RNA-Templates bis zu 2 kb. cDNA-Synthese und PCR-Verstärkung werden im selben Röhrchen ohne Öffnen zwischen den einzelnen Verfahren durchgeführt. Dieses optimierte System bietet maximale RT-PCR-Effizienz, Empfindlichkeit und Spezifität.

Supplier: Enzo Life Sciences

AMPIGENE® 1-Step RT-PCR Kit for optimised and efficient cDNA synthesis and PCR in a single tube.

Supplier: AGILENT

Provides the highest efficiency of conversion of RNA to cDNA, fully optimised for qPCR applications.

Supplier: Abcam

cDNA Kit ab286905 (previously known as Novo cDNA Kit M1165-100) contains A Moloney-Murine Leukemia Virus Reverse Transcriptase With Genetic Modifications To Abolish Rnase H Activity To Achieve Thermal Stability.

Supplier: Quantabio

Faster, better, tougher next generation cDNA SuperMix for cDNA synthesis.

Supplier: OriGene

qSTAR first-strand cDNA synthesis kit for miRNA is a two-step protocol kit.

Supplier: Thermo Fisher Scientific

The RevertAid™ First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from RNA templates. The kit is suitable for synthesis of cDNA up to 13 kb. The kit uses RevertAid™ Reverse Transcriptase which has lower RNase H activity compared to AMV reverse transcriptase. The recombinant RiboLock™ RNase Inhibitor, supplied with the kit, effectively protects RNA template from degradation. It is fully compatible with reverse transcription reaction, as it maintains activity at temperatures up to 55 °C.

Supplier: Thermo Fisher Scientific

The Maxima™ First Strand cDNA Synthesis Kit is a convenient system optimised for use as a first step in two step RT-qPCR. The kit includes the Maxima™ Reverse Transcriptase, which is an advanced enzyme derived by in vitro evolution of M-MuLV RT for RT-qPCR application. The enzyme features high thermostability, robustness and increased synthesis rates in RT-qPCR.

Supplier: Thermo Fisher Scientific

Thermo Scientific™ Maxima™ H Minus cDNA Synthesis Master Mix provides all cDNA synthesis reaction components in a convenient one-tube master mix. It is optimised for highly efficient cDNA synthesis for two-step quantitative RT-PCR (RT-qPCR) applications.

Supplier: Thermo Fisher Scientific

The First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from mRNA or total RNA templates. The kit uses M-MuLV Reverse Transcriptase, which has lower RNase H activity compared to AMW reverse transcriptase. The enzyme maintains activity at 37 °C and is suitable for synthesis of cDNA up to 9 kb. The recombinant Thermo Scientific RiboLock RNase Inhibitor, supplied with the kit, effectively protects RNA from degradation at temperatures up to 55 °C.

Supplier: Thermo Fisher Scientific

RevertAid™ H Minus First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from RNA templates. The kit includes RevertAid™ H Minus Reverse Transcriptase, which has a point mutation that completely eliminates RNase H activity. Therefore, it does not degrade RNA in RNA-DNA hybrids during synthesis of the first strand cDNA.

Supplier: Thermo Fisher Scientific

Maxima™ H Minus First Strand cDNA Synthesis Kit is a complete system for highly efficient synthesis of first strand cDNA. The kit uses the Maxima™ H Minus Reverse Transcriptase (RT), which is an advanced enzyme derived by in vitro evolution of M-MuLV RT. The enzyme features the highest thermostability among the derivatives of M-MuLV RT and lacks RNase H activity. The Maxima™ H Minus First Strand cDNA Synthesis Kit allows synthesis of long cDNAs up to 20 kb at elevated temperatures (up to 65 °C), superseding other systems' abilities to produce full-length cDNA. Due to increased synthesis rates the reaction can be completed in 30 minutes.

Supplier: Thermo Fisher Scientific

RevertAid™ Reverse Transcriptase (RT) is a genetically modified M-MuLV RT. It differs from the M-MuLV RT by its structure and catalytic properties. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity and a RNase H activity specific to RNA in RNA-DNA hybrids, which is significantly lower than that of Avian Myeloblastosis Virus (AMV) reverse transcriptase.