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Sie haben gesucht nach: qPCR/RT-qPCR Enzyme und Kits

Taq extender PCR additive

Supplier: AGILENT

Improves reliability and yield of conventional Taq-based PCR amplifications.

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Viability PCR starter kits

Supplier: Biotium

Kits designed to make it simple to start performing viability PCR with the choice of viability dye (PMA or PMAxx™).

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AMPIGENE® qPCR 1-Step Probe Kit No-ROX

Supplier: ENZO LIFE SCIENCES

Optimised and efficient cDNA synthesis and probe-based qPCR in a single tube.

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PerfeCTa® qPCR ToughMix®

Supplier: Quantabio

PerfeCTa® qPCR ToughMix® ist ein 2-fach konzentrierter gebrauchsfertiger Reaktionscocktail für die PCR-Amplifikation von DNA-Templates. Er widersteht vielen bekannten Inhibitoren von PCR, die sich häufig in Rohproben aus dem Umweltbereich, in Pflanzen- oder Tiergeweben finden. Es ist ein vielseitiges Echtzeit-qPCR-Reagenz mit maximaler Empfindlichkeit und PCR-Effizienz mit zahlreichen fluorogenen Fühlerchemien wie TaqMan® Hydrolysefühlern. PerfeCTa® qPCR ToughMix® enthält alle erforderlichen Reaktionskomponenten, mit Ausnahme von Primern, Fühler(n) und DNA-Template. Die hellblaue Farbe des AccuVue™ Farbmarkers erleichtert den Reaktionsaufbau in weißen oder transparenten Platten und minimiert Pipettierungs- und Mischfehler. Er hat keine negative Auswirkung auf die qPCR-Leistung oder die Stabilität des Produkts.

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qScript™ XLT One-Step-RT-qPCR ToughMix®

qScript™ XLT One-Step-RT-qPCR ToughMix®

Supplier: Quantabio

qScript™ XLT One-Step-RT-qPCR ToughMix® ist ein gebrauchsfertiger, hochempfindlicher Mastermix für die reverse Transkription quantitativer PCR (RT-qPCR) von RNA-Templates.

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Brilliant III Probe MM, Agilent Technologies

Brilliant III Probe MM, Agilent Technologies

Supplier: AGILENT

Brilliant III QPCR and QRT-PCR master mixes increase overall sensitivity and speed through the novel, quick-activating hot start and the engineered Taq-mutant.

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Brilliant II probe MM

Supplier: AGILENT

Brilliant II QPCR and QRT-PCR reagents were developed for improved sensitivity of detection, ensuring reproducible quantification even at low target concentrations.

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Brilliant II SYBR® Master mix

Supplier: AGILENT

Brilliant II SYBR® QPCR and QRT-PCR reagents were developed for improved sensitivity of detection, ensuring reproducible quantification even at low target concentrations.

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qPCR SuperMix, PerfeCTa® MultiPlex

Supplier: Quantabio

Dieser SuperMix ist ein zweifach konzentrierter, gebrauchsfertiger Reaktionscocktail für qRT-qPCR, mit allen Komponenten abgesehen von Primern, Sonden und Templates. Das System überschreitet die Multiplex-Grenzen von herkömmlichen PCR MasterMixes und ermöglicht die eindeutige Amplifikation von bis zu fünf Zielmolekülsequenzen in einem einzelnen Röhrchen. Die Unterdrückung von Ampliconen mit wenigen Kopien durch Referenzmoleküle mit vielen Kopien während der Amplifikation ist ein häufiges Problem bei der Multiplex-PCR. Dadurch kann die scheinbare Darstellung und Quantifizierung von Zielmolekülsequenzen mit wenigen Kopien verzerrt oder maskiert werden. PerfeCTa® MultiPlex qPCR SuperMix bietet einen dynamischen Bereich und eine Empfindlichkeit gegenüber einer Multiplex-qPCR, die vergleichbar mit denjenigen von Singleplex-qPCR-Fühler-Assays sind, ohne dass dabei begrenzte oder variable Primerkonzentrationen erforderlich sind.

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AMPIGENE® qPCR 1-Step Probe Kit Hi-ROX

Supplier: ENZO LIFE SCIENCES

Optimised and efficient cDNA synthesis and probe-based qPCR in a single tube.

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Ultra-Plex™ 1-Step ToughMix® (4X)

Ultra-Plex™ 1-Step ToughMix® (4X)

Supplier: Quantabio

Ultra-Plex™ 1-Step ToughMix® is a ready-to-use, 4X-concentrated master mix for reverse transcription quantitative PCR (RT-qPCR) of RNA templates using hybridisation probe detection chemistries such as TaqMan® 5’-hydrolysis probes on Real-Time PCR systems that utilise ROX passive reference with 490 nM excitation. First-strand cDNA synthesis and PCR amplification are carried out in the same tube without opening between procedures. It is ideal for highly sensitive quantification of RNA viruses or low abundance RNA targets in uni- or multiplexed RT-qPCR applications as well as high throughput gene-expression studies. The system has been optimised to deliver maximum RT-qPCR efficiency, sensitivity, and specificity in reduced reaction volumes and fast cycle times. Ultra-Plex™ 1-Step ToughMix® contains all required components for RT-qPCR except RNA template and probe. It is compatible with all dual-labeled probe chemistries.

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PerfeCTa® MultiPlex qPCR ToughMix®

PerfeCTa® MultiPlex qPCR ToughMix®

Supplier: Quantabio

PerfeCTa® MultiPlex qPCR ToughMix® ist ein 5-fach konzentrierter gebrauchsfertiger Reaktionscocktail für quantitative Echtzeit-PCR (qPCR). Er widersteht vielen bekannten Inhibitoren von PCR, die sich häufig in Rohproben aus dem Umweltbereich, in Pflanzen- oder in Tiergeweben finden. Er enthält alle Komponenten außer Primer, Sonden und Schablonen. Der 5-fach konzentrierte ToughMix® ermöglicht das Hinzufügen größerer Mengen von Templates und eine verbesserte Erkennungsempfindlichkeit bei Proben mit geringer Konzentration. PerfeCTa® MultiPlex qPCR ToughMix® wurde für maximale PCR-Effizienz, Empfindlichkeit und Spezifität in reduzierten Reaktionsvolumina mit schnellen Zykluszeiten oder bei konventionellen PCR-Cycling-Protokollen optimiert.

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One-Step SYBR® Green qRT-PCR, qScript™

Supplier: Quantabio

qScript™ One-Step SYBR® Green qRT-PCR Kits reliably deliver maximum qRT-PCR efficiency, sensitivity and specificity in with a simplified protocol. These kits offer reliable and unbiased amplification of all RNA targets regardless of abundance. The proprietary supermix maximises activities of both reverse transcriptase and AccuStart™ Taq DNA polymerase without compromising specificity of amplification. qScript™ One-Step SYBR® Green qRT-PCR kits are available and optimised for all real-time PCR instrument platforms including those requiring normalisation with ROX reference dye.

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Brilliant III Ultra-Fast SYBR® green ROX QPCR master mix

Supplier: AGILENT

High performance, ultra-sensitive, SYBR® Green QPCR master mix reagent with ROX concentration for reliable quantification across a wide range of targets and templates.

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qPCR master mixes, Forget-Me-Not™ EvaGreen®

Supplier: Biotium

EvaGreen® Dye and high-performance dye-based qPCR master mixes containing EvaGreen® qPCR dye, Cheetah™ HotStart Taq Polymerase, and Forget-Me-Not™ tracking dye. Available with 2-colour tracking or pre-mixed with low or high ROX.

   Nachhaltige Optionen verfügbar
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qPCR master mixes, Fast Plus EvaGreen®

qPCR master mixes, Fast Plus EvaGreen®

Supplier: Biotium

A qPCR master mix containing Evagreen® qPCR dye and Cheetah™ Taq hotstart DNA polymerase, suitable for qPCR using a fast cycling protocol.

   Nachhaltige Optionen verfügbar
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AMPIGENE® qPCR 1-Step Probe Kit Lo-ROX

Supplier: ENZO LIFE SCIENCES

Optimised and efficient cDNA synthesis and probe-based qPCR in a single tube.

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TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *No ROX*

Supplier: AAT BIOQUEST

TAQuest™ FAST qPCR Master mix with Helixyte™ green is a ready-to-use 2X solution optimised for qPCR and two-step RT-qPCR.

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TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *High ROX*

Supplier: AAT BIOQUEST

TAQuest™ FAST qPCR Master mix with Helixyte™ green is a ready-to-use 2X solution optimised for qPCR and two-step RT-qPCR.

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TAMRA Dye qPCR Calibration Solution *10000X*

TAMRA Dye qPCR Calibration Solution *10000X*

Supplier: AAT BIOQUEST

TAMRA dye qPCR Calibration Solution supplied as a 10000X concentrate is compatible with a wide variety of qPCR instruments, including the ABI7500 Fast, QuantStudio™, and ViiA™ 7 systems

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Maxima™ SYBR® Green qPCR Master Mix (2X), with ROX

Maxima™ SYBR® Green qPCR Master Mix (2X), with ROX

Supplier: Thermo Fisher Scientific

The Maxima™ SYBR® Green qPCR Master Mix (2X) is a ready to use solution optimised for quantitative real time PCR and two-step real time RT-PCR on most real time PCR machines. The master mix includes Maxima™ hot start Taq DNA polymerase, SYBR® Green dye I and dNTPs in an optimised PCR buffer. ROX solution is provided in a separate vial for use with machines that require ROX as a passive reference dye. Maxima™ hot start Taq DNA polymerase in combination with the optimised buffer ensures PCR specificity and sensitivity. The SYBR® Green I intercalating dye allows for DNA detection and analysis without using sequence specific probes. dUTP is included in the mix for optional carryover contamination control using uracil-DNA glycosylase (UDG). The use of Maxima™ SYBR® Green qPCR Master Mix (2X) in real time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates.

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DyNAmo™ ColorFlash SYBR® Green qPCR Kits

DyNAmo™ ColorFlash SYBR® Green qPCR Kits

Supplier: FINNZYMES REAGENTS

DyNAmo™ qPCR Kits offer excellent performance in detection and quantitation of DNA and RNA sequences from various sources. The DyNAmo™ qPCR kit family features optimised kits for fast and conventional qPCR. Kits utilising either SYBR® Green or probe chemistries for various platforms are available. All DyNAmo™ qPCR kits are provided as convenient 2X master mixes - only template and primers need to be added.

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JUN Dye qPCR Calibration Solution *10000X*

JUN Dye qPCR Calibration Solution *10000X*

Supplier: AAT BIOQUEST

JUN dye qPCR Calibration Solution supplied as a 10000X concentrate is compatible with a wide variety of qPCR instruments, including the ABI7500 Fast, QuantStudio™, and ViiA™ 7 systems.

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VIC Dye qPCR Calibration Solution *10000X*

VIC Dye qPCR Calibration Solution *10000X*

Supplier: AAT BIOQUEST

VIC dye qPCR Calibration Solution supplied as a 10000X concentrate is compatible with a wide variety of qPCR instruments, including the ABI7500 Fast, QuantStudio™, and ViiA™ 7 systems.

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TAQuest™ qPCR Master Mix for TaqMan Probes *Low ROX*

Supplier: AAT BIOQUEST

TAQuest™ qPCR Master mix for TaqMan probes is a ready-to-use 2X solution optimised for qPCR and two-step RT-qPCR compatible with TaqMan gene expression assays.

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TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *Low ROX*

Supplier: AAT BIOQUEST

TAQuest™ FAST qPCR Master mix with Helixyte™ green is a ready-to-use 2X solution optimised for qPCR and two-step RT-qPCR.

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TAQuest™ qPCR Master Mix with Helixyte™ Green *Low ROX*

Supplier: AAT BIOQUEST

TAQuest™ qPCR Master mix with Helixyte™ green is a ready-to-use 2X solution optimised for qPCR and two-step RT-qPCR.

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TAQuest™ qPCR Master Mix with Helixyte™ Green *No ROX*

Supplier: AAT BIOQUEST

TAQuest™ qPCR Master mix with Helixyte™ green is a ready-to-use 2X solution optimised for qPCR and two-step RT-qPCR.

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TAQuest™ qPCR Master Mix for TaqMan Probes *High ROX*

Supplier: AAT BIOQUEST

TAQuest™ qPCR Master mix for TaqMan probes is a ready-to-use 2X solution optimised for qPCR and two-step RT-qPCR compatible with TaqMan gene expression assays.

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Maxima™ SYBR green/ROX QPCR master mix (2X)

Maxima™ SYBR green/ROX QPCR master mix (2X)

Supplier: Thermo Fisher Scientific

The Maxima™ SYBR® Green/ROX qPCR Master Mix (2X) is a ready to use solution optimised for quantitative real time PCR and two-step real time RT-PCR on most real time PCR machines. The master mix includes Maxima™ hot start Taq DNA polymerase, SYBR® Green dye I and dNTPs in an optimised PCR buffer and is supplemented with ROX passive reference dye. Only template and primers need to be added. Maxima™ hot start Taq DNA polymerase in combination with the optimised buffer ensures PCR specificity and sensitivity. The SYBR® Green I intercalating dye allows for DNA detection and analysis without using sequence specific probes. dUTP is included in the mix for optional carryover contamination control using uracil-DNA glycosylase (UDG). The use of Maxima™ SYBR® Green/ROX qPCR Master Mix (2X) in real time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates.

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