Sie haben gesucht nach: Enzyme und Kits für Endpunkt-PCR

Supplier: VWR Chemicals

Für eine erfolgreiche PCR ist ein optimales Puffersystem notwendig. VWR® Taq DNA Polymerase Kits enthalten generell zwei verschiedene Puffer, einen Haupt- und einen Zusatzpuffer, die sich für unterschiedliche PCR-Anforderungen eignen. Alle Puffer enthalten Tris und 15 mM MgCl₂ (1,5 mM MgCl₂ Endkonzentration). Für eine einfache Optimierung ist zudem MgCl₂ in einem separaten Röhrchen enthalten.

Supplier: VWR Chemicals

VWR® TEMPase Hot Start DNA-Polymerasen sind hochstabile Polymerasen mit höherer Spezifität, überlegener Empfindlichkeit und höheren Ausbeuten im Vergleich zu Standard-DNA Polymerasen. Diese Eigenschaften sorgen für beste Prozessivität gerade bei geringen DNA-Konzentrationen. Weitere Verwendungen umfassen Screening, Verstärkung von GC-reichen Sequenzen, Multiplex-PCR, Direct PCR und qPCR. Eine glyzerinfreie TEMPase Hot Start DNA-Polymerase für die Automatisierung und Gefriertrocknung ist ebenfalls erhältlich.

Supplier: VWR Peqlab

PCR-Produkte von bis zu 12 kb und bis zu 40 kb können mit den Hochleistungs-PCR-Systemen peqGOLD 'Mid Range' und' Long Range' jeweils sowohl für genomische und episomale Templates erreicht werden. Einsetzbar anstelle von Taq-DNA-Polymerasen in allenn PCR-Reaktionen, wenn große Fragmente mit hohen Erträgen und größter Genauigkeit amplifiziert werden sollen, einschließlich derCharakterisierungen von Sequenzen, die in λ-Phagen oder Cosmide kloniert wurden.

Supplier: VWR Chemicals

VWR® TEMPase Hot Start DNA Polymerases are highly stable polymerases, featuring higher specificity, superior sensitivity and greater yields compared to standard DNA polymerases. These features make them well suited for the detection of low abundance targets. Other uses include screening, amplification of GC-rich sequences, multiplex PCR, direct PCR and qPCR.

Supplier: VWR Chemicals

Taq DNA Polymerase is ideal for general PCR and primer extension reactions to amplify DNA templates with high sensitivity. PCR can be initiated from as few as 6 primed templates. Simply add 0,5 µl of Taq DNA Polymerase to a 25 µl PCR reaction.

Supplier: Quantabio

AccuStart™ Genotyping ToughMix® ist ein 2X konzentrierter, gebrauchsfertiger Reaktionscocktail für die Amplifikation von DNA-Templates, der Inhibitoren widersteht, die sich häufig in Rohproben aus dem Umweltbereich, in Pflanzen- oder Tiergeweben finden. Es handelt sich um ein robustes PCR-Reagenz, das eine hoch spezifische und effiziente Amplifikation für Genotypisierungs-Sequenzvariation ermöglicht, einschließlich allelischer Diskriminierung von Einzelnukleotid-Polymorphismen (SNP) unter Verwendung zahlreicher Fluorogensondensysteme wie TaqMan® Hydrolysefühlern.

Supplier: VWR Chemicals

Taq DNA polymerase is a thermostable recombinant DNA polymerase, which exhibits very high activity in primer extension and other molecular biology applications. The enzyme is isolated from thermus aquaticus and has a molecular weight of approximately 94 kDa.

Supplier: VWR Chemicals

Highly concentrated Taq DNA polymerase glycerol free 50 U/µl is ideal for industrial use and manufactures of diagnostic PCR kits.

Supplier: VWR Chemicals

VWR® Red Taq DNA Polymerase Master Mix, which also contains an inert red dye, can be directly loaded onto an agarose gel without addition of electrophoresis loading buffers.

Supplier: VWR Chemicals

VWR® Taq DNA Polymerase is an ultra-pure, thermostable, recombinant DNA polymerase, which provides robust PCR performance in a wide range of PCR applications, without time-consuming optimisation. The enzyme is isolated from Thermus aquaticus, and has a molecular weight of approximately 94 kDa. VWR® Taq DNA Polymerase has both a 5' to 3' DNA polymerase and a double strand 5' to 3' exonuclease activity. It leaves an A overhang, which makes the enzyme ideal for TA cloning.

Supplier: VWR Chemicals

VWR® Taq DNA Polymerase Glycerol Free 5U/µl is a thermostable recombinant DNA polymerase, which exhibits very high activity in primer extension and other molecular biology applications. The enzyme is isolated from Thermus aquaticus and has a molecular weight of approximately 94 kDa.

Supplier: VWR Chemicals

VWR® Taq Plus is an optimised format of Taq DNA polymerase master mix and, therefore, is a good alternative to Taq DNA polymerase and Taq DNA polymerase master mix.

Supplier: VWR Chemicals

The fast extract DNA solution provides rapid and efficient extraction of PCR-ready DNA from mammalian and plant tissues. DNA is ready in eight minutes. The one-reagent protocol is divided into two simple heating steps, which can be directly followed by PCR analysis, such as screening and genotyping.

Supplier: VWR Chemicals

This fast extract genotyping PCR kit consists of a fast extraction DNA solution and VWR® Red Taq DNA polymerase 2X master mix. It is ideal for genotyping of DNA extracted from mammalian tissues, plant material, saliva or bacteria, providing PCR-ready DNA in 8 minutes and genotyping results in less than 1,5 hours.

Supplier: VWR Chemicals

Fast HiFi DNA Polymerase 2 U/µl is a proofreading DNA polymerase displaying the following features; high fidelity >60X Taq DNA Polymerase, ability to amplify problematic DNA targets, such as those with low to high GC content and ability to perform amplification on long DNA targets. lt is recommended for applications, which require extremely high fidelity such as cloning / sub cloning, NGS applications and mutagenesis.

Supplier: VWR Chemicals

Fast HiFi DNA Polymerase 2 U/µl is a proof reading DNA polymerase displaying the following features; high fidelity >60X Taq DNA Polymerase, ability to amplify problematic DNA targets, such as those with low to high GC content and ability to perform amplification on long DNA targets. lt is recommended for applications, which require extremely high fidelity such as cloning/subcloning, NGS applications and mutagenesis.

Supplier: VWR Chemicals

Red Taq DNA Polymerase is a blend of Taq DNA polymerase combined with an inert red dye. The dye enables quick visual recognition of reactions to which enzyme has been added, as well as confirmation of complete mixing.

Supplier: VWR Chemicals

TEMPase Hot Start DNA Polymerase Master Mix and Blue TEMPase Master Mix are good alternatives to TEMPase Hot Start DNA Polymerase. These master mixes offer easy reaction assembly at room temperature, reduced set-up time and fewer handling steps, which lead to increased reproducibility. As a consequence TEMPase Hot Start DNA Polymerase Master Mix is highly suited to standard tests.

Supplier: VWR Chemicals

VWR® Taq DNA Polymerase Master Mix is a ready to use 1,1X or 2X reaction mix. Simply add primers, template and water to carry out primer extensions and other molecular biology applications.

Supplier: VWR Chemicals

Hot Start PCR-To-Gel Taq Master Mix, 2X is a ready to use reaction cocktail that contains all required components, except primers and template DNA, for routine PCR amplification of DNA fragments up to 4 kb.

Supplier: Quantabio

AccuStart™ II PCR ToughMix® is a 2X concentrated ready to use reaction cocktail for PCR amplification of DNA templates that overcomes many known inhibitors of PCR often present in crude samples extracted from environmental specimens, plant tissues, or animal tissues. It contains all components, except primers and template.

Supplier: AGILENT

The MycoSensor PCR and qPCR assay kits detect cultured Mycoplasma infection by real-time qPCR or PCR.

Supplier: Quantabio

PerfeCTa® PreAmp SuperMix ist ein 5-fach konzentrierter, gebrauchsfertiger Reaktionscocktail für die eindeutige, ausgewählte Anreicherung von Zielmolekülsequenzen aus begrenzenden Mengen von Ausgangsmaterial für Downstream-Genexpressions-Profiling oder gezielte erneute Sequenzierungen. Er enthält alle Komponenten, mit Ausnahme von Primern und Schablonen. Der 5-fach konzentrierte SuperMix ermöglicht auch höhere Schablonenvolumen bei der Arbeit mit Proben geringer Konzentration und/oder reduzierten Reaktionsvolumen. Der inerte hellblaue Farbmarker erleichtert die Darstellung kleiner Reaktionsvolumen und ermöglicht genaues Pipettieren.

Supplier: Quantabio

AccuStart™ II GelTrack PCR SuperMix is a 2X concentrated, ready-to-use reaction cocktail for routine PCR amplification of DNA fragments up to 4 kb followed by analysis on agarose gels. It contains all components, except primers and template. AccuStart™ II GelTrack PCR SuperMix simplifies reaction assembly, improves assay reproducibility, and reduces the risk of contamination. The supermix includes electrophoresis tracking dyes that migrate at approximately 4 kb and 50 bp to allow direct loading of PCR product on agarose gels following amplification. AccuStart™ II Taq DNA polymerase in the master mix is inactivated with monoclonal antibodies that bind the polymerase and keep it inactive prior to the initial PCR denaturation step. Upon heat activation (1 minute at 94 °C), the antibodies denature irreversibly, releasing fully active, unmodified Taq DNA polymerase. This enables specific and efficient primer extension with the convenience of room temperature reaction assembly.

Supplier: AGILENT

This DNA polymerase is ideal for a variety of techniques requiring high-fidelity DNA synthesis by the polymerase chain reaction (PCR).

Supplier: AGILENT

Cloned Pfu DNA Polymerase for high fidelity and difficult, GC-Rich PCR.

Supplier: AGILENT

Detect as low as 300 fg of Porcine DNA in food and pharmaceutical samples, including gel caps.

Supplier: ENZO LIFE SCIENCES

Increased sensitivity standard PCR mix for a broader range of samples, with enhanced speed, yield, and specificity.

Supplier: Quantabio

AccuStart™ II Taq DNA Polymerase is a high purity, recombinant Taq DNA polymerase preparation with high avidity monoclonal antibodies that bind the polymerase and keep it inactive prior to the initial PCR denaturation step. Upon heat activation (1 minute at 94 °C), the antibodies denature irreversibly, releasing fully active, unmodified Taq DNA polymerase. This enables specific and efficient primer extension with the convenience of room temperature reaction assembly. Non-specific extension of primers at low temperatures is a common cause of artifacts and poor sensitivity in PCR. The AccuStart™ II automatic hot-start enables specific and efficient primer extension in the PCR process with the added convenience of room temperature reaction assembly. The included 10X PCR Buffer II is a new optimised buffer that provides higher product yield, improved specificity, and enhanced multiplexing capability.

Supplier: AGILENT

PfuUltra High-Fidelity DNA Polymerase AD features a reformulated buffer system for increased economy with the same high performance as the original PfuUltra DNA Polymerase for robust, ultra-high-fidelity PCR.