Sie haben gesucht nach: Zellkultur-Ergänzungen und -Zusätze

Supplier: VWR Chemicals

Eigelb-Tellurit-Emulsion, VWR®

Supplier: VWR Chemicals

Beinhaltet eine Mischung von Protease-Inhibitoren: AEBSF, Aprotinin, E-64, Bestatin und Leupeptin. Jedes Gefäß kann in 1 ml deionisiertem Wasser zu einer 100X Lösung wiederhergestellt werden.

Supplier: VWR Chemicals

VWR® Trypsin-EDTA solutions are used in cell culture to remove adherent cells from a culture surface. Trypsin is a porcine pancreas-derived enzyme that is commonly used for the dissociation and disaggregation of anchorage-dependent mammalian cells and tissues. The concentration of trypsin necessary to separate cells from their substrate is dependent primarily on the cell type and the age of the culture. EDTA, a chelating agent, enhances enzymatic activity by removing calcium and magnesium ions. These ions obscure the peptide bonds on which trypsin acts as well as enhancing cell to cell adhesion.

Supplier: VWR Chemicals

Contains a mix of protease inhibitors: AEBSF, E-64, Bestatin, Pepstatin, Leupeptin and 1, 10-Phenanthroline. Each vial can be reconstituted in 1 ml dimethyl sulfoxide to form a 100X solution.

Supplier: VWR Chemicals

Der wasserlösliche Teil der autolysierten Hefe enthält einen Vitamin-B-Komplex.

Supplier: VWR Chemicals

Eigelb-Emulsion, steril, VWR®

Supplier: Biowest

L-Glutamin ist eine essentielle Aminosäure, die von praktisch allen in Kultur gezüchteten Säugetier- und Insektenzellen benötigt wird. Es ist ein entscheidender Bestandteil vieler Zellkulturmedien und dient als wichtige Energiequelle für Zellen in Kultur. L-Glutamin ist als Trockenpulver und als gefrorene Lösung sehr stabil. In flüssigen Medien oder Stammlösungen kann L-Glutamin jedoch relativ schnell abgebaut werden. L-Glutamin ist labiler in Zellkulturlösungen als andere Aminosäuren.

Supplier: PROTIDE PHARMACEUTICALS

Serum replacement with similar properties to TCM™ but contains additional components for cell types that have required higher concentrations of serum (typically more than 10%). TM-235™ is defined and multi-purpose. Contains highly purified, heat-treated bovine serum albumin, heat-treated bovine transferrin and recombinant insulin.

Supplier: G-Biosciences

Protease-PhosphataseArrest™ provides full protection of protein samples from proteases and phosphatases released during the preparation of cell and tissue lysates. Protease-PhosphataseArrest™ contains both irreversible and reversible protease inhibitors to inhibit serine, cysteine and other proteases along with five phosphatase inhibitors that target all the phosphatase categories: serine/threonine (Ser/Thr) specific, tyrosine specific and dual specificity phosphatases.

Supplier: MP Biomedicals

M9 Minimal medium salts are suitable for the preparation of M9 Minimal Medium for use in propagation of E.coli and plasmid amplification.

Supplier: HyClone products (Cytiva)

With HyrTryp™ cell dissociation reagent, an animal-derived component-free (ADCF) recombinant alternative to porcine trypsin, you can dissociate and harvest cells with confidence. Simply substitute it in your existing protocol.

Supplier: ENZO LIFE SCIENCES

Natural products are an unsurpassed source of chemical diversity and an ideal starting point for any screening program for pharmacologically active small molecules. Historically, natural products have been the most successful source of new drugs. The Screen-Well® natural product library offers over 500 isolated natural compounds of known structure and includes many compounds from ethnopharmacology. Compounds are provided in solution in DMSO. Individual compounds or subsets can be resupplied in gram quantities.

Supplier: G-Biosciences

A ready to use, 100X concentrated, broad range protease inhibitor cocktail that is fully compatible with 2D electrophoresis and subsequent mass spectrometry.

Supplier: G-Biosciences

The PhosphataseArrest™ phosphatase inhibitor cocktails are ready to use 100X solutions that are simply added to either extraction buffers or samples.

Supplier: G-Biosciences

A broad range, 100X concentrated, ready-to-use protease inhibitor cocktail, Plant ProteaseArrest™ inhibits plant serine, cysteine and other plant specific proteases including aminopeptidases, aspartic and metalloproteases.

Supplier: G-Biosciences

Protease inhibitor set contains 12 ready to use individual protease inhibitors, each at a 100X concentration. Various concentrations and/or combinations of protease inhibitors may be used to inhibit a broad spectrum of protease activity. The Protease inhibitor set can be used to design specific protease inhibitor cocktails, supplement existing cocktails or to screen for specific protease classes.

Supplier: HyClone products (Cytiva)

HyClone™ L-glutamine is used as a supplement in serum- and protein-free media. It supports the growth of cells that have high energy demands and synthesise large amounts of proteins and nucleic acids.

Supplier: ENZO LIFE SCIENCES

The Screen-Well® epigenetics library contains 43 compounds with defined activity against enzymes which carry out epigenetic modification of lysine. It also includes DNA methylation inhibitors. Compounds are dissolved in DMSO at 10 mM and aliquoted into deep-well plates at 100 µl or 500 µl per well. The library is a useful tool for chemical genomics, assay development and other pharmacological applications. The library contains inhibitors of these important enzymes: HDACs, SIRTs, Lysine demethylases, HATs, Histone methyl transferases, DNA methyltransferases as well as SIRT activators. A variety of structurally and mechanistically different compound classes are included.

Supplier: UPM Biomedicals

GrowDex®-T Hydrogel is a bio-friendly hydrogel extracted from birch and sourced from sustainable and responsibly managed forests.

Supplier: Biowest

Concentrated solutions of amino acids, vitamins or non essential amino acids can be added to a basal medium to enrich it and the resulting medium used as a classical MEM or BME. The final concentration of the MEM or BME must be 1X.

Supplier: Biowest

Phenol red is used as a pH indicator in cell culture. Its colour exhibits a gradual transition from yellow to red over the pH range 6,8 to 8,2. Above pH 8,2, phenol red turns a bright pink (fuchsia) colour.

Supplier: HyClone products (Cytiva)

Supplements cell culture with amino acids, vitamins, glucose, trace elements, and growth factors. Manufactured to meet cGMP manufacturing standards and QC specifications.

Supplier: ENZO LIFE SCIENCES

The Screen-Well® histaminergic and melatonin ligands plate contains 41 ligands including endogenous neurotransmitters, agonists, antagonists, and marketed drugs. The library is ideal for screening or identifying recombinant orphan G protein coupled receptors, target validation, secondary screening, assay development, and other pharmacological applications. The Histaminergic and Melatonin Ligands plate is 1 plate of the larger neurotransmitter library which contains over 680 ligands.

Supplier: HyClone products (Cytiva)

Dissociation reagents, such as trypsin, are used to detach and disaggregate adherent cells from the surface or substrate to which they are attached, as well as for dissociating tissues for primary cell culture.

Supplier: PROVITRO

Das Passagekit unmittelbar nach der Lieferung bei −20 °C im Dunkeln lagern. Vor Gebrauch das Passagekit auftauen. Nach dem Auftauen das Passagekit bei 2 °C bis 8 °C im Dunkeln maximal 4 Wochen lagern. Achtung: Nach dem Auftauen ist die Haltbarkeit auf 4 Wochen begrenzt.
Nach dem Auftauen kann das Passagekit bei 2 °C bis 8 °C im Dunkeln bis zu 1 Monat gelagert werden.. Das Medium nicht über 37 °C erhitzen oder unkontrollierbare Wärmequellen (z. B. Mikrowellengeräte) verwenden.
Frieren Sie das Kit nicht wieder ein. Dies kann zu hohen Salzkonzentrationen durch Ausfrieren von reinem Wasser führen, was zu irreversiblen Schäden führt.
Übermäßige Trypsinierung führt zu irreversiblen Schäden.
Überschreiten Sie aufgrund der tryptischen Aktivität dieses Passagekits nicht die empfohlene Inkubationszeit. Wenn die Zellen zu lange der Inkubation ausgesetzt werden, führt dies zu irreversiblen Schäden an Ihrer Kultur.

Supplier: PROTIDE PHARMACEUTICALS

General serum replacement for many cell types from a variety of species. TCM™ is a defined, multi-purpose serum replacement useful in studying the effects of growth factors and the production of proteins. TCM™ contains highly purified, heat-treated bovine serum albumin, heat-treated bovine transferrin and recombinant insulin.

Supplier: PROTIDE PHARMACEUTICALS

Material grade - USP, Ph. Eur.

Supplier: PROTIDE PHARMACEUTICALS

A unique cryopreservation solution that does not contain DMSO or foetal bovine serum. Cellvation™ is convenient to use and provides optimal recovery. Cells can be exposed to Cellvation™ for longer periods of time before the freezing process is initiated. Cellvation™ is ready to use and can be used for cells cultured in serum-free medium or with serum-containing medium.

Supplier: HyClone products (Cytiva)

Supplements cell culture with amino acids, vitamins, glucose, and trace elements and manufactured to meet cGMP manufacturing standards and QC specifications.

Supplier: ENZO LIFE SCIENCES

The Screen-Well® hepatotoxicity library is a focused collection of 238 compounds with defined and diverse hepatotoxicity, including steatosis, mitochondrial toxicity, Mallory body formation, cholestatic effects, and many more. A variety of structurally and mechanistically different compound classes are included, as well as nontoxic controls. The compounds are dissolved in DMSO at 10 mM and aliquoted into deep-well plates. The library is an essential tool for predictive toxicology screening and assay development.