Extraction buffers are provided ready to use and are effective in the isolation of total RNA and other proteins, improving protein yields and sample complexity from cells and tissues. Optimized extraction reagents enhance protein extraction in native or denaturing buffers compatible with downstream analytical techniques. An appropriate buffer solution added to a protein mixture during the extraction process can help improve the stability of protein molecules as these molecules are subjected to various forces designed to isolate them for study.

Protein extraction reagent, BugBuster® 10X, Millipore®

Supplier: Merck Millipore (Novagen)

BugBuster® 10X is a concentrated formulation of the proprietary detergents employed in BugBuster® without the addition of salts or buffer components. Concentrated BugBuster® provides a flexible alternative to the ready-to-use standard 1X BugBuster®, allowing user-defined dilution and addition of buffer components. BugBuster® 10X has all of the bioprocessing benefits of standard BugBuster® plus the freedom to control pH, reagent concentration, and buffer additives necessary for maximum extraction and activity of your target protein.

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Succinate, 0,2 M buffer solution, pH 5,0

Supplier: Thermo Fisher Scientific

Succinate, 0.2M buffer soln., pH 5.0, Thermo Fisher Scientific

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Triton® X-100 lysis buffer with glycerol (2X), pH 7,4

Supplier: Thermo Fisher Scientific

Triton® X-100 lysis buffer with glycerol (2X), pH 7.4, Thermo Fisher Scientific

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NP-40 lysis buffer (2X)

Supplier: Thermo Fisher Scientific

NP-40 lysis buffer (2X), Thermo Fisher Scientific

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Lysis buffers, red blood cells

Supplier: G-Biosciences

These DNase- and RNase-free buffers and reagents are designed for high quality research.

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MES lysis buffer with Triton® X-100

Supplier: Thermo Fisher Scientific

MES lysis buffer with Triton® X-100, Thermo Fisher Scientific

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PIPES lysis buffer with NP-40 (2X)

Supplier: Thermo Fisher Scientific

PIPES lysis buffer with NP-40 (2X), Thermo Fisher Scientific

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HEPES protein extraction buffer (5X)

Supplier: Thermo Fisher Scientific

Thermo Fisher Scientific

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ACK Lysing Buffer (1x), TheraPEAK™

Supplier: LONZA

Ammonium-Chloride-Potassium (ACK) Lysing Buffer is used to lyse red blood cells in preparations containing white blood cells.

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CSPL buffer

Supplier: OMEGA BIO-TEK

Component of the Mag-Bind® plant DNA 96 plus kit.

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2-D Protein Extraction Buffer

Supplier: Cytiva

Efficient cell lysis and protein extraction are key steps for achieving high-quality results in downstream applications. The 2-D protein extraction buffer trial kit is designed for those who experiment for establishing a suitable extraction and solubilization buffer for their protein samples.