Sie haben gesucht nach: Mikrobiologie-Lehrmittel

Supplier: EDVOTEK

In this experiment, students see a blue colour change in transformed cells due to the switching on of a gene. The pGAL plasmid gives a blue colour dye due to the production of the β-galactosidase protein by the lacZ gene. IPTG is not required in this experiment since pGAL contains the complete lacZ gene.

Supplier: EDVOTEK

In diesem Experiment nehmen transformierte Zellen ein Plasmid auf, welches das GFP-Gen (GFP: grün fluoreszierendes Protein) enthält. Das GFP-Gen wurde aus der Qualle Aequorea victoria isoliert. Die transformierten Kolonien, die das GFP-Protein enthalten, sind bei normalem Licht grün, fluoreszieren jedoch unter Langwellen-UV-Licht hell.

Supplier: EDVOTEK

Das grün fluoreszierende Protein (GFP) der Qualle Aequorea victoria wird in allen Bereichen der Wissenschaft sehr häufig eingesetzt. Viele Organismen sind mit dem GFP-Gen, der für die Biolumineszenz bei Quallen verantwortlichen Erbinformation, transformiert worden. Es hat sich so bewährt, dass Wissenschaftler durch Mutation blau fluoreszierendes Protein (BFP) hergestellt haben. In diesem einfachen Experiment transformieren die Studenten Bakterien entweder mit GFP, BFP oder beiden.

Supplier: EDVOTEK

Wenn zur Herstellung medizinisch brauchbarer Proteine durch Transformation Bakterien verwendet werden, müssen die fraglichen Proteine von allen anderen zellulären Proteinen getrennt werden. In diesem Experiment dienen die einmaligen Fluoreszenzeigenschaften des GFP und des BFP als Test im Verlauf der Aufreinigung der aus einem E. coli-Extrakt. Die Säulenfraktionen, die GFP oder BFP enthalten, werden durch Fluoreszenz identifiziert und anschließend aufgereinigt. Im Rahmen einer optionalen Übung können die aufgereinigten Proteinfraktionen durch SDS-Polyacrylamidgel-Elektrophorese (SDS-PAGE) getrennt werden, damit sich Reinheit und Größe der GFP und BFP einschätzen lassen.

Supplier: EDVOTEK

Educational Materials, Microbiology, Anwendung: Mikrobiologie

Supplier: G-Biosciences

Bacterial conjugation is a naturally occurring process that allows the transfer of DNA from one bacterium to another, which allows the transfer of genetic traits, particularly drug resistance. The kit contains two bacteria with different drug resistance genes and all the tools for students to study bacterial conjugation. This kit teaches the difference between bacterial genomic DNA and the transferable plasmid DNA and the mechanisms of bacterial conjugation. Students will also learn important basic microbiological techniques, including bacterial growth in liquid broth and on solid agar plates, antibiotic selection of bacteria and important aseptic techniques.

Supplier: EDVOTEK

Educational Materials, Microbiology, Anwendung: Mikrobiologie, ReadyPour™ LB-Agar mit Ampicillin

Supplier: G-Biosciences

The Gram staining method was first described in 1844 by the Danish bacteriologist Hans Christian Gram, after whom the test was named. The Gram staining test for bacteria is one of the most important tests in microbiology and is often one of the first tests performed in the identification of bacteria. The Gram staining method utilises the properties of bacterial cell walls and the stain crystal violet. Gram-positive microorganisms have a higher peptidoglycan and lower lipid content than Gram-negative bacteria in their cell walls and this makes them capable of retaining the Gram stain. This kit is supplied with two strains of bacteria and all the necessary components to carry out the Gram staining.

Supplier: G-Biosciences

This kit teaches aseptic handling techniques and cultivation of bacteria in liquid culture media and on solid phase agar plates. This kit is designed to educate students about the various stages of the bacterial growth cycle, i.e. lag, log or exponential, stationary and decline or death phases. The kit also teaches the importance of growing bacteria on solid phase agar plates to isolate single colonies of bacteria. This lab activity involves preparation of culture medium and solid agar plates.

Supplier: G-Biosciences

Antibiotics play a crucial role in the manipulation, screening and killing of bacteria in a range of biotechnology processes. This kit specifically teaches the basic principles of antibiotics, bacterial resistance and susceptibility. Students learn and understand the use of antibiotic resistance in screening for infectious diseases. Utilising a bacterial strain, students learn the effects of different antibiotics and visualise bacterial sensitivity and resistance to the supplied antibiotics. This method involves the use of filter paper discs impregnated with a specified concentration of antibiotics on the surface of an agar plate containing microbial cells. This kit will enable students to analyse the inhibitory effects of different antibiotics on selected bacterial cells and then determine which antibiotic is the most suitable to treat a bacterial infection.