Sie haben gesucht nach: Biologie-Lehrmittel

Supplier: EDVOTEK

Koronare Herzerkrankungen und Schlaganfälle sind die Haupttodesursachen in der westlichen Welt. Erhöhte Blutcholesterinwerte sind in diesen Fällen ein großer Risikofaktor. Die familiäre Hypercholesterolämie (FH), eine erblich bedingte Erkrankung, führt zu einem erhöhten Spiegel des „bösen“ Cholesterins, dem Low Density Lipoprotein (LDL, Lipoprotein niedriger Dichte). Bei unbehandelten Patienten mit dem mutierten FH-Gen kann die Erkrankung zum vorzeitigen Tod führen. In diesem Experiment wird die kolorimetrische Enzymreaktion vorgestellt, die die Grundlage für den klinischen Cholesterintest bildet. Außerdem können Studenten mit der Agarose-Gelelektrophorese ein simuliertes Gen-Screening für eine Krankheit analysieren.

Supplier: EDVOTEK

Investigate how agarose gel electrophoresis unlocks the color code used by food scientists to make colorful candies.

Supplier: EDVOTEK

Milk proteins are the most common food allergens in children. Accurate detection and labeling is vital to inform consumers about potentially dangerous foods.

Supplier: EDVOTEK

In this experiment, students see a blue colour change in transformed cells due to the switching on of a gene. The pGAL plasmid gives a blue colour dye due to the production of the β-galactosidase protein by the lacZ gene. IPTG is not required in this experiment since pGAL contains the complete lacZ gene.

Supplier: EDVOTEK

In diesem Experiment nehmen transformierte Zellen ein Plasmid auf, welches das GFP-Gen (GFP: grün fluoreszierendes Protein) enthält. Das GFP-Gen wurde aus der Qualle Aequorea victoria isoliert. Die transformierten Kolonien, die das GFP-Protein enthalten, sind bei normalem Licht grün, fluoreszieren jedoch unter Langwellen-UV-Licht hell.

Supplier: EDVOTEK

Das grün fluoreszierende Protein (GFP) der Qualle Aequorea victoria wird in allen Bereichen der Wissenschaft sehr häufig eingesetzt. Viele Organismen sind mit dem GFP-Gen, der für die Biolumineszenz bei Quallen verantwortlichen Erbinformation, transformiert worden. Es hat sich so bewährt, dass Wissenschaftler durch Mutation blau fluoreszierendes Protein (BFP) hergestellt haben. In diesem einfachen Experiment transformieren die Studenten Bakterien entweder mit GFP, BFP oder beiden.

Supplier: EDVOTEK

Wenn zur Herstellung medizinisch brauchbarer Proteine durch Transformation Bakterien verwendet werden, müssen die fraglichen Proteine von allen anderen zellulären Proteinen getrennt werden. In diesem Experiment dienen die einmaligen Fluoreszenzeigenschaften des GFP und des BFP als Test im Verlauf der Aufreinigung der aus einem E. coli-Extrakt. Die Säulenfraktionen, die GFP oder BFP enthalten, werden durch Fluoreszenz identifiziert und anschließend aufgereinigt. Im Rahmen einer optionalen Übung können die aufgereinigten Proteinfraktionen durch SDS-Polyacrylamidgel-Elektrophorese (SDS-PAGE) getrennt werden, damit sich Reinheit und Größe der GFP und BFP einschätzen lassen.

Supplier: G-Biosciences

The final goal in molecular biology is often the expression of a recombinant protein. The transformed plasmids can be used as templates by the bacteria to produce protein. Students learn about essential promoters and other elements necessary for successful protein expression in bacteria, including the differences between inducible and constitutive (unregulated) expression.The Expression of a Recombinant Protein kit allows students to express a protein either constitutively or under the control of an inducible promoter, which is activated with IPTG (isopropyl-beta-D-thiogalactopyranoside).

Supplier: G-Biosciences

The kit utilises the principle of diffusion of nucleic acids on a nylon membrane to determine their concentration. No spectrophotometers required.

Supplier: G-Biosciences

This lab activity involves analysis of an enzyme reaction using a specific enzyme substrate and inhibitor. Students study how the rate of enzyme reaction is dependent on substrate concentration and the influence of agents (inhibitors) and conditions that affects the enzyme reaction such as pH and temperature.

Supplier: G-Biosciences

Following restriction enzyme digestion of DNA molecules, researchers need to rejoin the ends of the DNA to generate recombinant DNA, a process known as ligation. Ligation of DNA is achieved with the bacterial enzyme T4 DNA ligase, which catalyses the formation of phosphodiester bonds. The DNA Ligation kit teaches students about ligation as they ligate several DNA fragments together to make larger pieces of DNA that are easily identified by agarose electrophoresis.

Supplier: G-Biosciences

In this lab activity, students learn to perform simple protein isolation procedures to isolate the protein fingerprint from various fresh tissues. They will compare the protein fingerprints of 4 different tissues to understand that the function of a particular organ is due to the proteins that are localised to the specific organ. Also included in this kit are four dried protein samples (from mouse liver, brain, heart and lungs) to compare as a control, if fresh tissues cannot be obtained.

Supplier: G-Biosciences

A major challenge for molecular biologists and genetic engineers is to easily detect and analyze genetic mutations that occur naturally, causing diseases, or during genetic engineering or cloning, whether deliberate or accidental. Students learn about different types of genetic mutations, including substitutions, deletions and point mutations and about various techniques used to detect genetic mutations. The kit contains all the reagents to screen simulated clinical samples for a mutant gene, using both the polymerase chain reaction (PCR) and restriction digestion mapping. Students conduct a simple clinical diagnostic experiment in order to identify a diseased patient.

Supplier: G-Biosciences

In this lab activity, students learn to perform carefully controlled experiments to generate protein fragments using a proteolysis enzyme and then analyse the protein fragments by electrophoresis. By analysis of protein fragmentation patterns, i.e. protein fingerprints, students learn about protein sequence, structure, and their conservation. Students resolve a set of 3 functionally identical protein samples selected from throughout the animal kingdom; including human, bovine and sheep. After generating fingerprints by electrophoresis, students examine the protein fingerprint of each sample to determine the degree of conservation.

Supplier: G-Biosciences

This lab activity has two objectives - to deepen the understanding of protein molecules and for students to learn the potential of electrophoresis in protein analysis. Students study the fundamentals of protein structure from their primary structure to the more complex tertiary and quaternary structures, utilising protein electrophoresis. Complex mixtures of protein samples and characterised pure protein samples, in conjunction with electrophoresis, are utilised to study protein structure and the potential of protein electrophoresis. Using non-denaturing and denaturing electrophoresis, students understand the difference between primary, tertiary and quaternary structures, the importance of disulfhydryl bridges in maintaining protein structure and electrophoresis in studying complete proteins and protein subunits.

Supplier: G-Biosciences

Using the Protein Electrophoresis kit, students will learn the principles of various types of electrophoresis, including denaturing and non-denaturing electrophoresis, and how this powerful technique is used to analyse proteins. The kit will introduce students to the different separation matrices currently in use and will understand their differing separation properties and their role in protein analysis. Students have an option of casting their own electrophoresis gels using polyacrylamide or using pre-cast commercially available gels. This kit is provided with all of the reagents, buffers and supplies needed for casting acrylamide gels, preparing protein samples, running electrophoresis, and staining the gels for visualisation of protein bands. Test protein samples and protein standards are also provided.

Supplier: G-Biosciences

Biodiversity lab activity is designed for determination of protein contents and biomass in diverse biological samples to study how biomass is related to biodiversity in nature. Students collect and catalogue plant leaf samples from a diverse group of locally available plants. This lab activity involves determination of natural weight of each plant sample, grinding a predetermined amount of each sample, and the subsequent extraction of proteins from the samples. Students then learn to determine protein contents of each plant sample and attempt to relate the protein content with biomass. Students in this lab activity are challenged to think, analyse, and seek answers as to why protein biomasses vary for a given natural weight for different plants. Finally, they will relate that finding to the biodiversity of nature.

Supplier: G-Biosciences

This lab activity is designed to study highly specific lock-key matching properties of antigen-antibody and how this highly specific interaction can be exploited as a tool for research and analysis. This study involves the use of an immunodiffusion technique in which antigen and antibody are allowed to diffuse in a solid agarose medium. When antigen and antibody meet, antigen-antibody complex is formed, which leads to precipitation. Antigen-antibody precipitate is formed in the zone where the concentration of the two matching pairs reaches an optimal known as the zone of equivalence, which results in formation of a visible opaque precipitate region in agarose medium. Those regions of precipitation can be used for determination of concentration or titer of both antigen and antibody. This Antigen-Antibody Interaction kit is a hands-on study of both Ouchterlony Double Diffusion and Radial Immunodiffusion techniques. This kit also provides additional guidance materials for teaching other types of antigen-antibody interactions concepts such as immunoelectrophoresis and immunoprecipitation.

Supplier: G-Biosciences

Determination of protein concentration is an essential technique in all aspects of protein studies and proteomics. The Assays for Protein Quantification kit includes three of the most widely used protein assays and allows for a direct comparison of the three assays that teaches students the benefits and limitations of each assay. Each assay is available individually to allow teaching of a specific assay, without the option of comparing and contrasting with other assays. The three assays covered are the Biuret Protein Assay, Lowry Protein Assay and the Coomassie Blue Dye Protein Assay. The Assays for Protein Quantification kit provides all the reagents required to perform both protein assays, including protein standards for accurate quantification, in a single lab activity. An often underestimated factor in quantifying protein is the presence of non-protein interfering agents, such as salts and detergents. This kit teaches students about common laboratory agents that affect the protein assays, the reasoning behind their interferences and how to overcome the interference. Students also learn how to select a protein assay for different applications.

Supplier: G-Biosciences

Bacterial conjugation is a naturally occurring process that allows the transfer of DNA from one bacterium to another, which allows the transfer of genetic traits, particularly drug resistance. The kit contains two bacteria with different drug resistance genes and all the tools for students to study bacterial conjugation. This kit teaches the difference between bacterial genomic DNA and the transferable plasmid DNA and the mechanisms of bacterial conjugation. Students will also learn important basic microbiological techniques, including bacterial growth in liquid broth and on solid agar plates, antibiotic selection of bacteria and important aseptic techniques.

Supplier: EDVOTEK

Educational Materials, Microbiology, Anwendung: Mikrobiologie

Supplier: EDVOTEK

Educational Materials, Microbiology, Anwendung: Mikrobiologie, ReadyPour™ LB-Agar mit Ampicillin

Supplier: EDVOTEK

Link important STEM concepts using Agarose Gel Electrophoresis.

Supplier: EDVOTEK

Hands-on simulation of CRISPR-Cas9 to target and treat Cystic Fibrosis.

Supplier: EDVOTEK

Hands-on simulation of molecular testing for COVID-19.

Supplier: G-Biosciences

This kit teaches aseptic handling techniques and cultivation of bacteria in liquid culture media and on solid phase agar plates. This kit is designed to educate students about the various stages of the bacterial growth cycle, i.e. lag, log or exponential, stationary and decline or death phases. The kit also teaches the importance of growing bacteria on solid phase agar plates to isolate single colonies of bacteria. This lab activity involves preparation of culture medium and solid agar plates.

Supplier: G-Biosciences

The Gram staining method was first described in 1844 by the Danish bacteriologist Hans Christian Gram, after whom the test was named. The Gram staining test for bacteria is one of the most important tests in microbiology and is often one of the first tests performed in the identification of bacteria. The Gram staining method utilises the properties of bacterial cell walls and the stain crystal violet. Gram-positive microorganisms have a higher peptidoglycan and lower lipid content than Gram-negative bacteria in their cell walls and this makes them capable of retaining the Gram stain. This kit is supplied with two strains of bacteria and all the necessary components to carry out the Gram staining.

Supplier: G-Biosciences

Antibiotics play a crucial role in the manipulation, screening and killing of bacteria in a range of biotechnology processes. This kit specifically teaches the basic principles of antibiotics, bacterial resistance and susceptibility. Students learn and understand the use of antibiotic resistance in screening for infectious diseases. Utilising a bacterial strain, students learn the effects of different antibiotics and visualise bacterial sensitivity and resistance to the supplied antibiotics. This method involves the use of filter paper discs impregnated with a specified concentration of antibiotics on the surface of an agar plate containing microbial cells. This kit will enable students to analyse the inhibitory effects of different antibiotics on selected bacterial cells and then determine which antibiotic is the most suitable to treat a bacterial infection.

Supplier: 3B Scientific

Educational Materials, Physiology and Health, Anwendung: Biologie, Poster, Drogenabhängigkeit, laminiert

Supplier: 3B Scientific

Educational Materials, Physiology and Health, Anwendung: Biologie, Poster, Alkoholabhängigkeit, laminiert