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Supplier: EDVOTEK

Kit for teaching students how to use a micropipette with ease and accuracy using multi-coloured dyes.

Supplier: EDVOTEK

In this experiment, students see a blue colour change in transformed cells due to the switching on of a gene. The pGAL plasmid gives a blue colour dye due to the production of the β-galactosidase protein by the lacZ gene. IPTG is not required in this experiment since pGAL contains the complete lacZ gene.

Supplier: EDVOTEK

Das grün fluoreszierende Protein (GFP) der Qualle Aequorea victoria wird in allen Bereichen der Wissenschaft sehr häufig eingesetzt. Viele Organismen sind mit dem GFP-Gen, der für die Biolumineszenz bei Quallen verantwortlichen Erbinformation, transformiert worden. Es hat sich so bewährt, dass Wissenschaftler durch Mutation blau fluoreszierendes Protein (BFP) hergestellt haben. In diesem einfachen Experiment transformieren die Studenten Bakterien entweder mit GFP, BFP oder beiden.

Supplier: EDVOTEK

Wenn zur Herstellung medizinisch brauchbarer Proteine durch Transformation Bakterien verwendet werden, müssen die fraglichen Proteine von allen anderen zellulären Proteinen getrennt werden. In diesem Experiment dienen die einmaligen Fluoreszenzeigenschaften des GFP und des BFP als Test im Verlauf der Aufreinigung der aus einem E. coli-Extrakt. Die Säulenfraktionen, die GFP oder BFP enthalten, werden durch Fluoreszenz identifiziert und anschließend aufgereinigt. Im Rahmen einer optionalen Übung können die aufgereinigten Proteinfraktionen durch SDS-Polyacrylamidgel-Elektrophorese (SDS-PAGE) getrennt werden, damit sich Reinheit und Größe der GFP und BFP einschätzen lassen.

Supplier: EDVOTEK

This experiment introduces chromatographic theory and methods of thin layer chromatography (TLC). A mixture of dyes are separated on a cellulose-based TLC plate using different solvent systems.

Supplier: EDVOTEK

Most molecules have a net charge within a pH range of 2 to 10. When the pH is altered, the net charge on molecules can change drastically. In this experiment, a mixture of 2 chemicals is absorbed onto a solid support ion-exchange column and separated during elution under conditions that influence their net charge.

Supplier: EDVOTEK

In diesem Experiment nehmen transformierte Zellen ein Plasmid auf, welches das GFP-Gen (GFP: grün fluoreszierendes Protein) enthält. Das GFP-Gen wurde aus der Qualle Aequorea victoria isoliert. Die transformierten Kolonien, die das GFP-Protein enthalten, sind bei normalem Licht grün, fluoreszieren jedoch unter Langwellen-UV-Licht hell.

Supplier: EDVOTEK

This experiment introduces chromatographic separation to the class and shows students how dyes of different colours separate on the basis of their size and shape. Columns may be rinsed and reused.

Supplier: G-Biosciences

This lab activity is designed to demonstrate the different classes of protein molecules and their classification based on solubility. Students learn fractionation of soluble, insoluble membrane proteins, and cytoskeleton proteins from a tissue sample. The insoluble protein fraction is further fractionated into hydrophilic and hydrophobic membrane proteins. Cell membrane structure and the role of hydrophobic membrane proteins are considered. This lab activity also provides an opportunity to understand characteristics of various classes of detergents and the role of detergents in solubilisation of hydrophobic membrane proteins.

Supplier: G-Biosciences

This DNA fingerprinting kit allows students to carry out their own criminal investigation by comparing DNA samples collected from suspects to DNA collected at a pseudo-crime scene.

Supplier: EDVOTEK

In this PCR experiment, students make billions of copies of a small amount of DNA in only 90 minutes. They just need to mix template DNA and primers with PCR beads that contain all of the other components required to carry out a PCR reaction. Students see the increasing amounts of DNA for themselves, taking samples every few cycles and analysing them on a DNA gel.

Supplier: EDVOTEK

In DNA fingerprinting, variable number tandem repeats (VNTR) are used to identify individuals. Using this kit, students type themselves at the D1S80 locus on chromosome 1. This region contains between 14 and 40 copies of a 16 base pair repeat.

Supplier: GLASWARENFABRIK KARL HECHT

Five glass tubes of different shape and inner dimensions. Down to each other. When filling the liquid in the pipes, it is clear that the height of the stowage in the tubes. pipes are independent of the design of the pipes. Comes with foot.

Supplier: G-Biosciences

The Physical Properties of Proteins kit is a lab activity that enables students to investigate the physical properties of several different proteins. Students will learn about protein solubility and how it is affected by various parameters; including temperature, pH, salt and dielectric constant. They will understand about protein precipitation due to pH, high salt and in the presence of organic solvents and about protein denaturation as a result of high temperature. In addition, the kit will demonstrate how non-protein agents, such as detergents drastically alter the physical properties of protein molecules and as a result, understand the importance of detergents in protein solubilisation. This lab activity involves analysis of three different types of pure proteins and then students alter some of those properties with a detergent and re-examine physical properties of those proteins. Students are challenged to consider how physical properties of protein molecules can be exploited for purification and characterisation of proteins and apply their findings on a test sample of complex tissue extract.

Supplier: GLASWARENFABRIK KARL HECHT

Five communicating glass tubes with different inner diameters. Used to show capillary forces and how they are affected by the inner diameter of the pipes.

Supplier: EDVOTEK

This experiment uses PCR to amplify a small section of Lambda DNA via a 2-step process, saving valuable classroom time and allowing completion of the lab in one session.

Supplier: EDVOTEK

Students can solve a crime using PCR. Plasmid DNA is provided that, when amplified by PCR, provides products that represent individual DNA profiles.

Supplier: EDVOTEK

This simulation experiment demonstrates the process of DNA amplification by PCR and how the amplified product is detected by separating the reaction mixture by agarose gel electrophoresis.

Supplier: EDVOTEK

Mit dem Ready-to-Load™ PCR-Kit werden Studenten in die Prinzipien und Anwendungen der Polymerasekettenreaktion (PCR) eingeführt. Das Simulationsexperiment enthält keine Human-DNA und erfordert keinen Thermocycler.

Supplier: EDVOTEK

Drinking water is routinely tested for contamination. If a screening tests positive, more sophisticated tests are required. One such test uses PCR in multiplex format. In this experiment, students test for the presence of three separate, classroom-safe organisms in a water sample using a single PCR reaction.

Supplier: EDVOTEK

Students use primers for a 300 base pair Alu insertion in chromosome 16 (PV92) to determine their own genotype. They can then compare their class results with others around the world over the internet.

Supplier: EDVOTEK

The mitochondria are thought to have evolved from a symbiotic relationship between prokaryotic and eukaryotic cells. Mitochondria have their own DNA and are only inherited via the maternal line. In this experiment, students amplify two regions of their mitochondrial DNA.

Supplier: G-Biosciences

Dot blotting is a simple technique to identify a known protein in a biological sample. The ease and simplicity of the technique makes dot blotting an ideal diagnostic tool.

Supplier: G-Biosciences

The Size Exclusion Chromatography kit teaches gel filtration or size exclusion chromatography and the use of this method in purification of biological samples. This method is based on separation of protein molecules based on their molecular size. This lab activity involves running size exclusion chromatography for separation of molecules based on their molecular sizes.

Supplier: G-Biosciences

Bacterial conjugation is a naturally occurring process that allows the transfer of DNA from one bacterium to another, which allows the transfer of genetic traits, particularly drug resistance. The kit contains two bacteria with different drug resistance genes and all the tools for students to study bacterial conjugation. This kit teaches the difference between bacterial genomic DNA and the transferable plasmid DNA and the mechanisms of bacterial conjugation. Students will also learn important basic microbiological techniques, including bacterial growth in liquid broth and on solid agar plates, antibiotic selection of bacteria and important aseptic techniques.

Supplier: MACHEREY-NAGEL

Advanced kit for science education.

Supplier: G-Biosciences

The Affinity Chromatography kit teaches the basic principle of affinity chromatography utilising highly specific affinity columns. This lab activity involves preparation of a crude protein extract and running affinity exchange chromatography for isolation of a protein.

Supplier: EDVOTEK

ABO and Rh typing of blood left at the scene of a crime can help to narrow down a list of suspects. In this experiment, students use agglutination to identify the blood group of unknown blood samples as a step to identify a criminal.

Supplier: EDVOTEK

Ready-to-Load™ kit for teaching DNA fingerprinting and to show how DNA evidence is used in modern forensics. This experiment allows for varied results depending on the selection of DNA fingerprinting patterns.

Supplier: EDVOTEK

Die grundlegenden Konzepte des genetischen Fingerabdrucks werden anhand dieses Ready-to-Load™ Kits erläutert, DNA vom Tatort wird mit der DNA von Verdächtigen verglichen. Fingerabdruckmuster werden mittels Agarose-Gelelektrophorese separiert, und die Studenten erarbeiten, wer das Verbrechen begangen haben könnte.