Vektoren, Plasmide und Bibliotheken
Bei der molekularen Klonierung sind Vektoren DNA-Moleküle, die zur künstlichen Übertragung von fremdem Genmaterial in eine andere Zelle eingesetzt werden. Die vier wichtigsten Arten von Vektoren sind Plasmide, virale Vektoren, Cosmide und künstliche Chromosomen, wobei die Plasmide am häufigsten verwendet werden. Vektoren können zur Klonierung eingesetzt werden, und einige Vektoren sind speziell auf diesen Verwendungszweck zugeschnitten, während andere für die Transkription und Proteinexpression vorgesehen sind. Einfachere Transkriptionsvektoren werden zur Amplifikation des eingefügten genetischen Materials verwendet.
Slc7a11 (NM_011990) mouse tagged ORF clone
Supplier: OriGene
Slc7a11 (GFP-tagged) - Mouse solute carrier family 7 (cationic amino acid transporter y+ system) member 11 (Slc7a11), (10 µg).
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E. coli BL21
Supplier: Cytiva
The E. coli BL21 strain is protease-deficient for optimal expression of a variety of recombinant proteins.
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M13KO7 Helper Phage, Cytiva
Supplier: Cytiva
M13 phage for the GST expression system.
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Polyadenylic acid (poly-A)
Supplier: Cytiva
Poly(A) (polyadenylic acid) can be used to evaluate the binding of potential therapeutic agents to mRNA in drug development research.
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Expression vectors, pGEX
Supplier: Cytiva
The pGEX vectors have an expanded multiple cloning site (MCS) that contains six restriction sites. The expanded MCS facilitates the unidirectional cloning of cDNA inserts obtained from libraries constructed using many available lambda vectors. pGEX-6P-1, pGEX-6P-2, and pGEX-6P-3 each encode the recognition sequence for site-specific cleavage by PreScission Protease between the GST domain and the multiple cloning site. pGEX-4T-1, pGEX-4T-2, and pGEX-4T-3 are derived from pGEX-2T and contain a Thrombin recognition site. pGEX-5X-1, pGEX-5X-2, and pGEX5X-3 are derivatives of pGEX-3X and possess a Factor Xa recognition site.
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Gaussia Luc vector for luciferase assays, expression vectors, Pierce™
Supplier: Thermo Fisher Scientific
Gaussia Luc vectors contain a gene cloned from the copepod, Gaussia princeps. The gene encodes a naturally-secreted bioluminescent Gaussia luciferase (approx. 20 kDa), which enables measurement of the reporter activity in media (for real-time assays) and in cell lysates.
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Expression vectors, cypridina Luc vector for luciferase assays, Pierce™
Supplier: Thermo Fisher Scientific
Cypridina Luc vectors contain a gene cloned from the marine ostracod, Cypridina noctiluca.
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Expression vectors, green renilla Luc vector for luciferase assays, Pierce™
Supplier: Thermo Fisher Scientific
Green Renilla Luc vectors contain a gene cloned from the sea pansy, Renilla reniformis
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Expression vectors, red firefly Luc vector for Luciferase assays, Pierce™
Supplier: Thermo Fisher Scientific
Red Firefly Luc vectors contains a mutant form of the firefly luciferase gene that has a red-shifted emission spectrum.
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Pierce™ Gaussia-Dura Luc-Vektor für Luciferase-Assays, Expressionsvektoren
Supplier: Thermo Fisher Scientific
Gaussia Dura Luc-Vektoren enthalten eine mutierte Form des Gaussia Luciferase-Gens, das eine bessere Stabilität des Biolumineszenzsignals verleiht als native Luciferase. Gaussia-Dura-Luciferase (ca. 20 kDa) ist ein sezerniertes Protein, das die Messung der Reporteraktivität in Medien (für Echtzeit-Assays) und in Zelllysaten ermöglicht.
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Vector, pBR322, MP Biomedicals
Supplier: MP Biomedicals
pBR322 is a 4363 bp vector purified from Escherichia coli. It is one of the most commonly used cloning vectors, that confers resistance to ampicillin and tetracycline. It contains single restriction sites for Pst I and Pvu I within the ampicillin-resistance gene (ampr) and single restriction sites for BamH I, Sal I, Sph I, EcoR V, Nru I and Xma III within the tetracycline-resistance gene (tetr).
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Vector, pUC 18, MP Biomedicals
Supplier: MP Biomedicals
pUC 18 is a small vector of approximately 2,7 Kb containing the Pvu II/EcoR I fragment of pBR322 and the lacZ gene multiple cloning site from M13mp18.
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Cell-free protein synthesis, rapid translation system (RTS) vectors
Supplier: biotechrabbit
Expression vectors for PCR-based generation of linear templates.
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#: 20864.320
Anbieter: VWR Chemicals
#: 83640.320
Anbieter: VWR Chemicals
#: 31770.294
Anbieter: VWR Chemicals
#: 1.00030.2500
Anbieter: Merck
#: 83645.320
Anbieter: VWR Chemicals
#: 20060.320
Anbieter: VWR Chemicals