Vektoren, Plasmide und Bibliotheken

Supplier: Merck Millipore (Novagen)

The pSTBlue-1 AccepTor™ vector kits are designed to simplify the PCR cloning process by using non-proofreading thermostable DNA polymerases (eg. KOD XL polymerase and native and recombinant Taq polymerases), which leave single 3'-dA overhangs on the reaction products. The AccepTor™ vectors enable direct ligation by virtue of single 3'-dU overhangs that anneal efficiently with 3'-dA extensions on PCR products. The dU residues are converted to dT residues in vivo following transformation.

Supplier: Cytiva

The pGEX vectors have an expanded multiple cloning site (MCS) that contains six restriction sites. The expanded MCS facilitates the unidirectional cloning of cDNA inserts obtained from libraries constructed using many available lambda vectors. pGEX-6P-1, pGEX-6P-2, and pGEX-6P-3 each encode the recognition sequence for site-specific cleavage by PreScission Protease between the GST domain and the multiple cloning site. pGEX-4T-1, pGEX-4T-2, and pGEX-4T-3 are derived from pGEX-2T and contain a Thrombin recognition site. pGEX-5X-1, pGEX-5X-2, and pGEX5X-3 are derivatives of pGEX-3X and possess a Factor Xa recognition site.

Supplier: Cytiva

The E. coli BL21 strain is protease-deficient for optimal expression of a variety of recombinant proteins.

Supplier: Cytiva

M13 phage for the GST expression system.

Supplier: Thermo Fisher Scientific

Gaussia Dura Luc-Vektoren enthalten eine mutierte Form des Gaussia Luciferase-Gens, das eine bessere Stabilität des Biolumineszenzsignals verleiht als native Luciferase. Gaussia-Dura-Luciferase (ca. 20 kDa) ist ein sezerniertes Protein, das die Messung der Reporteraktivität in Medien (für Echtzeit-Assays) und in Zelllysaten ermöglicht.

Supplier: Thermo Fisher Scientific

Green Renilla Luc vectors contain a gene cloned from the sea pansy, Renilla reniformis

Supplier: MP Biomedicals

pUC 18 is a small vector of approximately 2,7 Kb containing the Pvu II/EcoR I fragment of pBR322 and the lacZ gene multiple cloning site from M13mp18.

Supplier: MP Biomedicals

pBR322 is a 4363 bp vector purified from Escherichia coli. It is one of the most commonly used cloning vectors, that confers resistance to ampicillin and tetracycline. It contains single restriction sites for Pst I and Pvu I within the ampicillin-resistance gene (ampr) and single restriction sites for BamH I, Sal I, Sph I, EcoR V, Nru I and Xma III within the tetracycline-resistance gene (tetr).

Supplier: Cytiva

Poly(A) (polyadenylic acid) can be used to evaluate the binding of potential therapeutic agents to mRNA in drug development research.

Supplier: Thermo Fisher Scientific

Cypridina Luc vectors contain a gene cloned from the marine ostracod, Cypridina noctiluca.

Supplier: Thermo Fisher Scientific

Red Firefly Luc vectors contains a mutant form of the firefly luciferase gene that has a red-shifted emission spectrum.

Supplier: Thermo Fisher Scientific

Gaussia Luc vectors contain a gene cloned from the copepod, Gaussia princeps. The gene encodes a naturally-secreted bioluminescent Gaussia luciferase (approx. 20 kDa), which enables measurement of the reporter activity in media (for real-time assays) and in cell lysates.

Supplier: biotechrabbit

Expression vectors for PCR-based generation of linear templates.