Vektoren, Plasmide und Bibliotheken
Bei der molekularen Klonierung sind Vektoren DNA-Moleküle, die zur künstlichen Übertragung von fremdem Genmaterial in eine andere Zelle eingesetzt werden. Die vier wichtigsten Arten von Vektoren sind Plasmide, virale Vektoren, Cosmide und künstliche Chromosomen, wobei die Plasmide am häufigsten verwendet werden. Vektoren können zur Klonierung eingesetzt werden, und einige Vektoren sind speziell auf diesen Verwendungszweck zugeschnitten, während andere für die Transkription und Proteinexpression vorgesehen sind. Einfachere Transkriptionsvektoren werden zur Amplifikation des eingefügten genetischen Materials verwendet.
InterPlay adenoviral TAP system
Supplier: AGILENT
The InterPlay Mammalian TAP system allows you to recover interacting proteins from mammalian cells.
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Lentivirale Kontrollpartikel
Supplier: OriGene
Lenti-Partikel tragen eine 29-Mer-Scrambled-Sequenzkassette und dienen als Negativkontrolle.
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Human Tagged ORF-Klon, Androgenrezeptor (AR) (NM_000044)
Supplier: OriGene
Der ORF-Klon wird über eine Ionenaustauschsäule gereinigt und in einem 2D-Barcode-Matrixröhrchen mit 10 µg transfektionsbereiter, getrockneter Plasmid-DNA (mit 100 µl Wasser rekonstituieren) versandt.
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sparQ mRNA-Seq Kit
Supplier: Quantabio
Hochempfindlicher mRNA-Bibliotheksvorbereitungs-Workflow mit effizienter Poly(A)-Erfassung.
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Human Tagged ORF Clone, SPCA2 (ATP2C2)
Supplier: OriGene
ATP2C2 (tGFP-tagged) - Human ATPase, Ca++ transporting, type 2C, member 2 (ATP2C2), transcript variant 3.
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ORF Clones, GABA B receptor 1 (GABBR1), human tagged, TrueORF®
Supplier: OriGene
GABA B receptor 1 (GABBR1), human tagged ORF clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
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Human Untagged FBXL17 Clone
Supplier: OriGene
FBXL17 (untagged)-Human F-box and leucine-rich repeat protein 17 (FBXL17). The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10 µg of transfection-ready, dried plasmid DNA (reconstitute with 100 µl of water).
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Cell-free protein expression vectors, T7, mammalian
Supplier: Thermo Fisher Scientific
The T7 cell-free expression vectors (pT7CFE1) are cloning plasmids optimised for use with the Thermo Scientific™ 1-Step Human In Vitro Protein Expression System. They are used for the in vitro translation (IVT) of genes into functional proteins when used in combination with the 1-Step Human IVT system. The pT7CFE1 vector is available with single or tandem affinity tags at the N- or C- terminus to facilitate protein purification and detection. The pT7CFE vectors are suitable for insertion of cloned genes, cDNAs, ORFs or PCR products for in vitro transcription and translation.
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Transfection control plasmids, pVectOZ
Supplier: OZ BIOSCIENCES
pVectOZ transfection plasmids are engineered in an optimised backbone and are ideal for all transfections.
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pET-14b DNA, Novagen®
Supplier: Merck Millipore (Novagen)
Der pET-14b-Vektor trägt eine N-terminale His·Tag®-Sequenz, gefolgt von einer Thrombin-Spaltstelle und drei Klonierungsstellen.
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Vector, pET-24d(+) DNA
Supplier: Merck Millipore (Novagen)
The pET-24a-d(+) vectors carry an N-terminal T7·Tag® sequence plus an optional C-terminal His·Tag® sequence.
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Vector, pET-24(+) DNA
Supplier: Merck Millipore (Novagen)
pET-24(+) is a transcription vector designed for expression from bacterial translation signals carried within a cloned insert. It lacks the ribosome binding site and ATG start codon present on the pET translation vectors. A C-terminal His·Tag® sequence is available
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Slc7a11 (NM_011990) mouse tagged ORF clone
Supplier: OriGene
Slc7a11 (GFP-tagged) - Mouse solute carrier family 7 (cationic amino acid transporter y+ system) member 11 (Slc7a11), (10 µg).
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Expression vectors, pET-41 DNA, Novagen®
Supplier: Merck Millipore (Novagen)
pET-41 series is designed for cloning and high-level expression of peptide sequences fused with the 220 aa GST•Tag™ protein. The cloning/expression region of the coding strand transcribed by T7 RNA polymerase. The f1 origin is oriented so that infection with helper phage will produce virions containing single stranded DNA that corresponds to the coding strand.
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pET-30a(+) DNA, Novagen®
Supplier: Merck Millipore (Novagen)
The pET-30a-c(+) vectors carry an N-terminal His Tag®/thrombin/S Tag™/enterokinase configuration plus an optional C-terminal His tag sequence.
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pET-24b(+) DNA, Novagen®
Supplier: Merck Millipore (Novagen)
The pET-24a-d(+) vectors carry an N-terminal T7-Tag® sequence plus an optional C-terminal His-Tag® sequence. These vectors differ from pET-21a-d(+) only by their selectable marker (kanamycin vs ampicillin resistance).
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