Kompetente Zellen

Kompetente Zellen sind Zellen, deren Membran durchlässiger gemacht wurde, damit sie Fremd-DNA besser aufnehmen können. Das Verfahren zur Herstellung kompetenter Zellen umfasst eine Calciumchlorid- und Hitzeschock-Behandlung, wobei Zellen, die sich in der logarithmischen Wachstumsphase befinden, effizienter kompetent gemacht werden können als Zellen in anderen Wachstumsphasen. Faktoren wie Transformationseffizienz, genetische Marker und Verpackungsformat wirken sich auf die für Ihr Projekt erforderliche Zeit und Arbeit aus, ebenso wie auf den Erfolg des Projekts. Um ein optimales Ergebnis zu gewährleisten, stehen mehrere Zelltypen und Formate zur Auswahl.

Protein expression competent cell pack

Supplier: AGILENT

Protein Expression competent cell packs efficiently test multiple strains for expression.

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SCS110 competent cells

Supplier: AGILENT

SCS110 competent cells for generating unmethylated DNA improve quality and yield of miniprep DNA.

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Competent cells, ArcticExpress

Supplier: AGILENT

Tackle two common bacterial gene expression hurdles: protein insolubility and codon bias.

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EletroTen-blue electroporation-competent cells

Supplier: AGILENT

Achieve the highest transformation efficiencies available when cloning large plasmids and ligated DNA.

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TG1 electroporation-competent cells

Supplier: AGILENT

Phage display libraries from electroporation-competent cells with high transformation efficiency.

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SCSI supercompetent cells

Supplier: AGILENT

SCSI supercompetent and DH5α competent cells are classic E. coli strains engineered for competence.

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SURE competent cells

Supplier: AGILENT

SURE 2 competent cells are ideal for routine cloning of DNA with secondary structures.

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XL-1 red competent cells

Supplier: AGILENT

XL-1 Red Competent Cells are ideal for introducing random mutations into a cloned gene of interest.

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DH5α Chemikalisch Kompetent E. coli Küvetten

Supplier: OriGene

Competent Cells, DH5α chemisch kompetente Zellen (≥10⁸ KBE/μg pUC19-DNA)

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XL10-Gold ultracompetent cells

Supplier: AGILENT

XL10-Gold ultracompetent cells transform large plasmids and ligated DNA with highest efficiency.

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JM competent cells

Supplier: AGILENT

JM Competent Cells are recombination- and E. coli K12 restriction-proficient.

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BJ5183 Electroporation-competent cells

Supplier: AGILENT

BJ5183 electroporation competent cells are a recombination proficient bacterial strain.

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Competent cell packs

Supplier: AGILENT

High efficiency competent cells in convenient packaging designed to satisfy higher throughput requirements.

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BL21 competent cells

Supplier: AGILENT

BL21 competent cells are an all-purpose strain for high-level protein expression and easy induction.

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XL1 blue and XL2 blue competent cells

Supplier: AGILENT

XL1 and XL2-Blue Competent Cells are versatile for routine cloning and allow blue-white colour screening for high efficiency cloning of methylated DNA.

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TK competent cells

Supplier: AGILENT

Easily induce and express phosphorylated proteins, which can be used to screen expression libraries, to affinity purify or blot proteins that interact with the tyrosine-phosphorylated target.