Sie haben gesucht nach: Puffer
Die gegen pH-Änderungen beständigen Puffer schützen Proben bei Zugabe von Säuren oder Basen. In Form von Pulvern oder Flüssigkeiten werden sie effizient zur elekrophoretischen Isolierung und Trennung von Proteinen eingesetzt. Die salzhaltigen Puffer können auch zur Kalibrierung und Standardisierung von pH-Messgeräten verwendet werden, um genaue Messergebnisse zu gewährleisten. Blocking-Puffer verhindern die Anbindung von Antikörpern an freie Membran-Bindungsstellen, die ansonsten einen störenden Hintergrund verursachen würden. Die Puffer sind je nach Bedarf in verschiedenen Konzentrationen sowie in flüssiger oder getrockneter Form erhältlich.
Proteasome assay buffer
Supplier: ENZO LIFE SCIENCES
Assay buffer from the 20S Proteasome Assay Kit for Drug Discovery.
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10X Wash Buffer PT
Supplier: Abcam
10X Wash Buffer PT (ab206977) is part of the reagents, controls and accessories range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.
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Cathepsin S Assay Buffer
Supplier: ENZO LIFE SCIENCES
50mM potassium phosphate buffer, pH 6,5, 50 mM NaCl, 2 mM EDTA, 0,01% Triton X-100®, 0,5 mM DTT.
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Protein blocking buffers, Superior™
Supplier: G-Biosciences
Blocking buffer is an enhanced blocking agent available in multiple formats. Superior™ Blocking Buffer contains a proprietary antigenically non-determinant protein for blocking Western blot membranes, blocking and storage of ELISA plates, and blocking prior to immunohistochemistry staining.
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Immunoassay Blocking (BSA Free)
Supplier: Abcam
Immunoassay Blocking (BSA Free) (ab171535) maintains the activity of antibodies and other biomolecules adsorbed or immobilized to solid substrates.
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Dry buffer packs, Carbonate-Bicarbonate Buffer, JAW™
Supplier: G-Biosciences
Premixed buffer powders that simply require adding deionised water to generate a ready-to-use buffer.
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Plasmid DNA buffers
Supplier: OMEGA BIO-TEK
Solution 3, neutralisation buffer for silica spin column methods
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HEPES Pufferlösung (1 mol/l)
Supplier: Cayman Chemical
HEPES Pufferlösung (1 mol/l)
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Neutralising reagent
Supplier: ENZO LIFE SCIENCES
Neutralising buffer for low pH solutions and for use with cyclic nucleotide and isoprostane kits.
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Background suppressor system (Permeabilising), TrueBlack® IF
Supplier: Biotium
A buffer set that blocks non-specific antibody binding as well as direct interaction of fluorescent dyes on antibodies with cells or tissue sections.
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IHC PBS tablets
Supplier: ENZO LIFE SCIENCES
Solid. 100 ml PBS (1X) tablets containing 137 mM sodium chloride, 2.7 mM potassium chloride, and 10 mM phosphate buffer.
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Tween-20 solution (10%)
Supplier: ENZO LIFE SCIENCES
This Tween®-20 solution (10%) is specially designed to create the Blocking Buffer and Assay Buffer in ImmunoSet products. Each bottle is sufficient for use with 5×96 wells microtiter plates if following the instructions in our ImmunoSet protocol.
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Lysepuffer, hypotonisch
Supplier: VWR Chemicals
Detergenzienfreier Puffer für die Isolierung von Proteinen aus Gewebekulturzellen. Zelllyse und die anschließende Isolierung der rohen zytoplasmischen Fraktion sind in weniger als 30 Minuten abgeschlossen.
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Blocking buffers, FirstChoice™
Supplier: G-Biosciences
FirstChoice™ Blocking Buffer is ideal as a first choice for optimization of new assays and systems, or when determining the optimal blocking buffer for elimination of non specific binding sites in ELISA, blotting, immunohistochemistry and other applications. Buffers are conveniently supplied in widely used TBS (Tris-buffered saline at pH 7,5) and PBS (phosphate-buffered saline at pH 7,5) buffers as well as in separate formulations containing Tween® 20 for improving blocking efficiencies.
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Semi Dry Blot Transfer buffer (10X)
Supplier: Thermo Fisher Scientific
Elektrophorese-Puffer, Transfer buffer, Semi-dry Blot Transfer Buffer, 10X
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Tris-Borate-EDTA (TBE) buffer, 10X
Supplier: PanReac AppliChem
10X konzentrierte Lösung aus 0,89 M Tris, 0,89 M Borsäure und 0,02 M EDTA-Na2, pH 8,3 ±0,2.
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Phosphate buffered saline (PBS), 10X
Supplier: G-Biosciences
PBS 10X solution is used as a wash buffers for Western blotting, ELISA and other applications.
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Hybridisation buffers
Supplier: G-Biosciences
The hybridisation buffers like Church & Gilbert’s, Denhardt solution, hybridisation denaturing solution, hybridisation neutralising solution, SSC, SSPE, TMAC Hyb solution and TNT Buffer are used in wide range of applications. Church & Gilbert’s Hybridization Buffer is prepared from molecular biology grade SDS (sodium dodecyl sulfate), monosodium phosphate (NaHPO₄), EDTA (ethylenediaminetetracetic acid) and bovine serum albumin (BSA) Fraction V using DNase, RNase and Protease-free water. It consists of 1mM EDTA, 1% BSA, 0,5M sodium phosphate monobasic, and 7% SDS (at a pH of 7,2).
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TISAB III-Lösung (Gesamtionenstärkenpuffer) für die Fluoridbestimmung, Supelco®
Supplier: Merck
TISAB III-Lösung (Gesamtionenstärkenpuffer)
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Phosphatpuffer, pH 6.6 bei 25 °C
Supplier: SIGMA ALDRICH MICROSCOPY
Die Pufferkapazität des Phosphatpuffers ist pH-empfindlich und wird vorzugsweise bei physiologischem pH-Wert (7,2 bis 7,4) verwendet.
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Puffer, TRIS, 1,0-M-Lösungen, 0,2 µm gefiltered
Supplier: Thermo Fisher Scientific
Farblose, geruchslose, gefilterte TRIS-Puffer.
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ISA (ionic strength adjustment solution: 1 M KCl), Supelco®
Supplier: Merck
Solution used to establish a constant ionic strength in a sample solution containing e.g. calcium for det. with ISE.
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TISAB IV Solution for Determination of Fluoride, Supelco®
Supplier: Merck
Total ionic strength adjustment buffers (TISAB), is generally used for the microdetermination of fluorides.
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IHC Wash Buffer, Enzo Life Sciences
Supplier: ENZO LIFE SCIENCES
The IHC wash buffer is a for washing and removing excess reagents during immunostaining.
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PBS-TWEEN pH 7.5, ultrarein
Supplier: VWR Chemicals
Phosphate Buffered Saline (PBS) with 0,05% Tween® 20, pH 7,5 is a ready-to-use 1× concentration.
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PBS/Tween® Pufferkonzentrat, 20X
Supplier: VWR Chemicals
PBS Tween® 20 blot wash buffer is a 20X concentration with 1% Tween® 20.
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SSC buffer, 20X concentrate, pH 7,0
Supplier: Rockland Immunochemicals
Saline-sodium citrate (SSC) buffer is most commonly used as a buffer for the hybridisation process and washing in experiments where spcies of nucleic or ribonucleic acid are to be matched to complimentary sequences (such as Southern and Nouthern blotting, in situ hybridisation, or DNA microarrays). SSC is used to help control the stringency of hybridisation in these steps.
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Cesium chloride-lanthanum chloride buffer solution acc. to Schinkel for atomic absorption spectroscopy (AAS), Supelco®
Supplier: Merck
10 g/L CsCl + 100 g/L La in dilute hydrochloric acid.
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TISAB I Solution, Supelco®
Supplier: Merck
TISAB, in general, is mixed with samples, as it helps in maintaining the ionic strength and pH constant. It is mainly used to decomplex metal-F complexes.