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732 results for Puffer

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Puffer

Die gegen pH-Änderungen beständigen Puffer schützen Proben bei Zugabe von Säuren oder Basen. In Form von Pulvern oder Flüssigkeiten werden sie effizient zur elekrophoretischen Isolierung und Trennung von Proteinen eingesetzt. Die salzhaltigen Puffer können auch zur Kalibrierung und Standardisierung von pH-Messgeräten verwendet werden, um genaue Messergebnisse zu gewährleisten. Blocking-Puffer verhindern die Anbindung von Antikörpern an freie Membran-Bindungsstellen, die ansonsten einen störenden Hintergrund verursachen würden. Die Puffer sind je nach Bedarf in verschiedenen Konzentrationen sowie in flüssiger oder getrockneter Form erhältlich.

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Proteasome assay buffer

Supplier: ENZO LIFE SCIENCES

Assay buffer from the 20S Proteasome Assay Kit for Drug Discovery.

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HEPES Buffer pH 8.0 (1 M), Sterile

Supplier: Apollo Scientific

Aqueous solution

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10X Wash Buffer PT

10X Wash Buffer PT

Supplier: Abcam

10X Wash Buffer PT (ab206977) is part of the reagents, controls and accessories range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.

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Cathepsin S Assay Buffer

Supplier: ENZO LIFE SCIENCES

50mM potassium phosphate buffer, pH 6,5, 50 mM NaCl, 2 mM EDTA, 0,01% Triton X-100®, 0,5 mM DTT.

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Protein blocking buffers, Superior™

Protein blocking buffers, Superior™

Supplier: G-Biosciences

Blocking buffer is an enhanced blocking agent available in multiple formats. Superior™ Blocking Buffer contains a proprietary antigenically non-determinant protein for blocking Western blot membranes, blocking and storage of ELISA plates, and blocking prior to immunohistochemistry staining.

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Immunoassay Blocking (BSA Free)

Immunoassay Blocking (BSA Free)

Supplier: Abcam

Immunoassay Blocking (BSA Free) (ab171535) maintains the activity of antibodies and other biomolecules adsorbed or immobilized to solid substrates.

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Dry buffer packs, Carbonate-Bicarbonate Buffer, JAW™

Supplier: G-Biosciences

Premixed buffer powders that simply require adding deionised water to generate a ready-to-use buffer.

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Plasmid DNA buffers

Supplier: OMEGA BIO-TEK

Solution 3, neutralisation buffer for silica spin column methods

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HEPES Pufferlösung (1 mol/l)

Supplier: Cayman Chemical

HEPES Pufferlösung (1 mol/l)

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Neutralising reagent

Supplier: ENZO LIFE SCIENCES

Neutralising buffer for low pH solutions and for use with cyclic nucleotide and isoprostane kits.

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Background suppressor system (Permeabilising), TrueBlack® IF

Background suppressor system (Permeabilising), TrueBlack® IF

Supplier: Biotium

A buffer set that blocks non-specific antibody binding as well as direct interaction of fluorescent dyes on antibodies with cells or tissue sections.

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IHC PBS tablets

Supplier: ENZO LIFE SCIENCES

Solid. 100 ml PBS (1X) tablets containing 137 mM sodium chloride, 2.7 mM potassium chloride, and 10 mM phosphate buffer.

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Tween-20 solution (10%)

Supplier: ENZO LIFE SCIENCES

This Tween®-20 solution (10%) is specially designed to create the Blocking Buffer and Assay Buffer in ImmunoSet products. Each bottle is sufficient for use with 5×96 wells microtiter plates if following the instructions in our ImmunoSet protocol.

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Lysepuffer, hypotonisch

Lysepuffer, hypotonisch

Supplier: VWR Chemicals

Detergenzienfreier Puffer für die Isolierung von Proteinen aus Gewebekulturzellen. Zelllyse und die anschließende Isolierung der rohen zytoplasmischen Fraktion sind in weniger als 30 Minuten abgeschlossen.

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Blocking buffers, FirstChoice™

Blocking buffers, FirstChoice™

Supplier: G-Biosciences

FirstChoice™ Blocking Buffer is ideal as a first choice for optimization of new assays and systems, or when determining the optimal blocking buffer for elimination of non specific binding sites in ELISA, blotting, immunohistochemistry and other applications. Buffers are conveniently supplied in widely used TBS (Tris-buffered saline at pH 7,5) and PBS (phosphate-buffered saline at pH 7,5) buffers as well as in separate formulations containing Tween® 20 for improving blocking efficiencies.

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Semi Dry Blot Transfer buffer (10X)

Supplier: Thermo Fisher Scientific

Elektrophorese-Puffer, Transfer buffer, Semi-dry Blot Transfer Buffer, 10X

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Tris-Borate-EDTA (TBE) buffer, 10X

Supplier: PanReac AppliChem

10X konzentrierte Lösung aus 0,89 M Tris, 0,89 M Borsäure und 0,02 M EDTA-Na2, pH 8,3 ±0,2.

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Phosphate buffered saline (PBS), 10X

Phosphate buffered saline (PBS), 10X

Supplier: G-Biosciences

PBS 10X solution is used as a wash buffers for Western blotting, ELISA and other applications.

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Hybridisation buffers

Hybridisation buffers

Supplier: G-Biosciences

The hybridisation buffers like Church & Gilbert’s, Denhardt solution, hybridisation denaturing solution, hybridisation neutralising solution, SSC, SSPE, TMAC Hyb solution and TNT Buffer are used in wide range of applications. Church & Gilbert’s Hybridization Buffer is prepared from molecular biology grade SDS (sodium dodecyl sulfate), monosodium phosphate (NaHPO₄), EDTA (ethylenediaminetetracetic acid) and bovine serum albumin (BSA) Fraction V using DNase, RNase and Protease-free water. It consists of 1mM EDTA, 1% BSA, 0,5M sodium phosphate monobasic, and 7% SDS (at a pH of 7,2).

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TISAB III-Lösung (Gesamtionenstärkenpuffer) für die Fluoridbestimmung, Supelco®

Supplier: Merck

TISAB III-Lösung (Gesamtionenstärkenpuffer)

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Phosphatpuffer, pH 6.6 bei 25 °C

Supplier: SIGMA ALDRICH MICROSCOPY

Die Pufferkapazität des Phosphatpuffers ist pH-empfindlich und wird vorzugsweise bei physiologischem pH-Wert (7,2 bis 7,4) verwendet.

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Puffer, TRIS, 1,0-M-Lösungen, 0,2 µm gefiltered

Supplier: Thermo Fisher Scientific

Farblose, geruchslose, gefilterte TRIS-Puffer.

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ISA (ionic strength adjustment solution: 1 M KCl), Supelco®

Supplier: Merck

Solution used to establish a constant ionic strength in a sample solution containing e.g. calcium for det. with ISE.

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TISAB IV Solution for Determination of Fluoride, Supelco®

Supplier: Merck

Total ionic strength adjustment buffers (TISAB), is generally used for the microdetermination of fluorides.

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IHC Wash Buffer, Enzo Life Sciences

Supplier: ENZO LIFE SCIENCES

The IHC wash buffer is a for washing and removing excess reagents during immunostaining.

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PBS-TWEEN pH 7.5, ultrarein

PBS-TWEEN pH 7.5, ultrarein

Supplier: VWR Chemicals

Phosphate Buffered Saline (PBS) with 0,05% Tween® 20, pH 7,5 is a ready-to-use 1× concentration.

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PBS/Tween® Pufferkonzentrat, 20X

PBS/Tween® Pufferkonzentrat, 20X

Supplier: VWR Chemicals

PBS Tween® 20 blot wash buffer is a 20X concentration with 1% Tween® 20.

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SSC buffer, 20X concentrate, pH 7,0

Supplier: Rockland Immunochemicals

Saline-sodium citrate (SSC) buffer is most commonly used as a buffer for the hybridisation process and washing in experiments where spcies of nucleic or ribonucleic acid are to be matched to complimentary sequences (such as Southern and Nouthern blotting, in situ hybridisation, or DNA microarrays). SSC is used to help control the stringency of hybridisation in these steps.

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TISAB I Solution, Supelco®

Supplier: Merck

TISAB, in general, is mixed with samples, as it helps in maintaining the ionic strength and pH constant. It is mainly used to decomplex metal-F complexes.

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