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Sie haben gesucht nach: Puffer

Puffer

Die gegen pH-Änderungen beständigen Puffer schützen Proben bei Zugabe von Säuren oder Basen. In Form von Pulvern oder Flüssigkeiten werden sie effizient zur elekrophoretischen Isolierung und Trennung von Proteinen eingesetzt. Die salzhaltigen Puffer können auch zur Kalibrierung und Standardisierung von pH-Messgeräten verwendet werden, um genaue Messergebnisse zu gewährleisten. Blocking-Puffer verhindern die Anbindung von Antikörpern an freie Membran-Bindungsstellen, die ansonsten einen störenden Hintergrund verursachen würden. Die Puffer sind je nach Bedarf in verschiedenen Konzentrationen sowie in flüssiger oder getrockneter Form erhältlich.

Buffers, sodium phosphate buffer solutions (0,5 mol/L)

Supplier: Cayman Chemical

Sodium phosphate stock solution (0,5 mol/L), pH-Wert: 8,0

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Buffered NaCl Peptone Solution pH 7,0

Supplier: LIOFILCHEM

Buffered NaCl Peptone Solution pH 7,0 is used for dissolving, suspending and diluting test samples. Its composition conforms to recommendations in the Harmonised Pharmacopoeias for the microbiological examination of non-sterile products.

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Blocking buffers, Protein-Free™

Supplier: G-Biosciences

Protein-Free™ Blocking buffer is a formulation of non-protein agents that eliminate non-specific binding sites in ELISA, blotting, immunohistochemistry and other applications. The absence of protein from the Protein-Free™ Blocking buffer eliminates problems associated with traditional protein based blockers, such as cross-reactivity and interference from glycosylated proteins.

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J.T.Baker® BAKER ANALYZED®, Buffer Solutions, Colour Coded

J.T.Baker® BAKER ANALYZED®, Buffer Solutions, Colour Coded

Supplier: Avantor

The quality of many BAKER ANALYZED™ reagents meets or exceeds the requirements set forth by the American Chemical Society (ACS).

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VWR® Buffer, Ammonium Chloride, pH 10, Reag. Ph.Eur.

VWR® Buffer, Ammonium Chloride, pH 10, Reag. Ph.Eur.

Supplier: VWR Chemicals

The reagent is specially intended for use in testing Ph Eur and monographs. This solution was produced according 4.1.1 of Ph Eur specifications using Ph Eur reagent quality components and water meeting the Ph Eur monograph requirement for purified water.

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Permeabilisation buffer

Permeabilisation buffer

Supplier: Biotium

Ready-to-use permeabilisation buffer for intracellular immunofluorescence staining for microscopy or flow cytometry.

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Fixation buffer for microscopy and flow cytometry

Fixation buffer for microscopy and flow cytometry

Supplier: Biotium

Ready-to-use formaldehyde-based fixation buffer for immunofluorescence staining for microscopy or flow cytometry.

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Kaliumphosphatpuffer (0,5 mol / l) Lösungen

Supplier: Cayman Chemical

Kaliumphosphat Pufferlösung (0,5 mol/l), pH-Wert: 8,0

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PBS (endotoxin-free), Enzo Life Sciences

Supplier: ENZO LIFE SCIENCES

Sterile and endotoxin-free PBS for use with TLRgrade™ or endotoxin-free grade reagents.

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TES buffer, 1X, ready to use, pH 7,8

Supplier: Rockland Immunochemicals

This product is ready-to-use as a working 1X solution and requires no further dilution. This buffer consists of 10 mM Tris HCl, 100 mM NaCl, and 1,0 mM EDTA at a pH of 7,8. Meticulously prepared using ultra pure reagents dissolved in DEPC treated pharmaceutical grade deionised water.

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Proteasome assay buffer

Supplier: ENZO LIFE SCIENCES

Assay buffer from the 20S Proteasome Assay Kit for Drug Discovery.

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Cathepsin S Assay Buffer

Supplier: ENZO LIFE SCIENCES

50mM potassium phosphate buffer, pH 6,5, 50 mM NaCl, 2 mM EDTA, 0,01% Triton X-100®, 0,5 mM DTT.

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HEPES Buffer Solution 1 M, HyClone™

HEPES Buffer Solution 1 M, HyClone™

Supplier: HyClone products (Cytiva)

Maintain physiological pH in cell culture, despite changes in carbon dioxide concentration (produced by cellular respiration) with Cytiva HyClone HEPES solution.

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HEPES Buffer pH 8.0 (1 M), Sterile

Supplier: Apollo Scientific

Aqueous solution

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10X Wash Buffer PT

10X Wash Buffer PT

Supplier: Abcam

10X Wash Buffer PT (ab206977) is part of the reagents, controls and accessories range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.

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RNA isolation buffers

Supplier: OMEGA BIO-TEK

RNA Wash Buffer I

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Optimizer Buffer™ for optimal cross-linking and modification

Optimizer Buffer™ for optimal cross-linking and modification

Supplier: G-Biosciences

The conjugation and modification reactions used to cross-link proteins or couple labels to proteins, such as biotin, enzymes, and fluorescent dyes, require certain conditions, including pH and chemical composition for optimal conjugation. Many common buffers routinely used in laboratories have an inhibitory effect on conjugation reactions; for example, Tris buffers inhibit coupling to amines.

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MMP-1 assay buffer

Supplier: ENZO LIFE SCIENCES

MMP-1 assay buffer

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Wash buffer concentrate

Supplier: ENZO LIFE SCIENCES

Liquid. TRIS buffered saline containing detergents.

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Plasmid DNA buffers

Supplier: OMEGA BIO-TEK

DNA wash buffer concentrate, low salt spin column was solution (makes 250 ml)

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HEPES Pufferlösung (1 mol/l)

Supplier: Cayman Chemical

HEPES Pufferlösung (1 mol/l)

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Tween-20 solution (10%)

Supplier: ENZO LIFE SCIENCES

This Tween®-20 solution (10%) is specially designed to create the Blocking Buffer and Assay Buffer in ImmunoSet products. Each bottle is sufficient for use with 5×96 wells microtiter plates if following the instructions in our ImmunoSet protocol.

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IHC PBS tablets

Supplier: ENZO LIFE SCIENCES

Solid. 100 ml PBS (1X) tablets containing 137 mM sodium chloride, 2.7 mM potassium chloride, and 10 mM phosphate buffer.

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Background suppressor system (Permeabilising), TrueBlack® IF

Background suppressor system (Permeabilising), TrueBlack® IF

Supplier: Biotium

A buffer set that blocks non-specific antibody binding as well as direct interaction of fluorescent dyes on antibodies with cells or tissue sections.

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Dry buffer packs, Carbonate-Bicarbonate Buffer, JAW™

Supplier: G-Biosciences

Premixed buffer powders that simply require adding deionised water to generate a ready-to-use buffer.

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Neutralising reagent

Supplier: ENZO LIFE SCIENCES

Neutralising buffer for low pH solutions and for use with cyclic nucleotide and isoprostane kits.

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Protein blocking buffers, Superior™

Protein blocking buffers, Superior™

Supplier: G-Biosciences

Blocking buffer is an enhanced blocking agent available in multiple formats. Superior™ Blocking Buffer contains a proprietary antigenically non-determinant protein for blocking Western blot membranes, blocking and storage of ELISA plates, and blocking prior to immunohistochemistry staining.

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Immunoassay Blocking (BSA Free)

Immunoassay Blocking (BSA Free)

Supplier: Abcam

Immunoassay Blocking (BSA Free) (ab171535) maintains the activity of antibodies and other biomolecules adsorbed or immobilized to solid substrates.

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Transfer or Electro Blotting buffer (10X), pH 8,5

Supplier: Thermo Fisher Scientific

Elektrophorese-Puffer, Transfer buffer, Transfer Electroblot Buffer 10X

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Blocking buffers, FirstChoice™

Blocking buffers, FirstChoice™

Supplier: G-Biosciences

FirstChoice™ Blocking Buffer is ideal as a first choice for optimization of new assays and systems, or when determining the optimal blocking buffer for elimination of non specific binding sites in ELISA, blotting, immunohistochemistry and other applications. Buffers are conveniently supplied in widely used TBS (Tris-buffered saline at pH 7,5) and PBS (phosphate-buffered saline at pH 7,5) buffers as well as in separate formulations containing Tween® 20 for improving blocking efficiencies.

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