Die gegen pH-Änderungen beständigen Puffer schützen Proben bei Zugabe von Säuren oder Basen. In Form von Pulvern oder Flüssigkeiten werden sie effizient zur elekrophoretischen Isolierung und Trennung von Proteinen eingesetzt. Die salzhaltigen Puffer können auch zur Kalibrierung und Standardisierung von pH-Messgeräten verwendet werden, um genaue Messergebnisse zu gewährleisten. Blocking-Puffer verhindern die Anbindung von Antikörpern an freie Membran-Bindungsstellen, die ansonsten einen störenden Hintergrund verursachen würden. Die Puffer sind je nach Bedarf in verschiedenen Konzentrationen sowie in flüssiger oder getrockneter Form erhältlich.
Pierce™ Fish Serum Blocking Buffer,Thermo Scientific
Supplier: Thermo Fisher Scientific
This Fish Serum blocking buffer is steelhead salmon serum in PBS that is especially useful as a blocking agent in IHC and other detection methods involving mammalian samples.
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Protein blocking solution, BLOK™ BLOTTO
Supplier: G-Biosciences
BLOK™ BLOTTO is a 5% novel modified milk protein solution used for Westerns, ELISA and dot blot blocking.
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SuperBlock™ Blocking Buffer
Supplier: Thermo Fisher Scientific
SuperBlock Blocking buffer is an optimised solution containing a single purified glycoprotein that provides incredibly fast and effective blocking for ELISA, IHC, and western blot analysis. Blocking micro-plates, membranes, or tissues with a SuperBlock buffer yields a high signal-to-noise ratio in most detection systems.
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Non-animal sera protein blocking Agent, FISH-Blocker™
Supplier: G-Biosciences
FISH-Blocker™ uses a fish protein as the primary blocking agent to eliminate cross reactivity. Using a non mammalian protein eliminates or minimises the interaction of antibodies raised in mammals. It is offered either in TBS (Tris-buffered saline) or in PBS (phosphate-buffered saline) at pH 7,5.
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StartingBlock™ Blocking Buffer
Supplier: Thermo Fisher Scientific
These StartingBlock™ blocking buffers are PBS and TBS formulations of a proprietary protein that provides exceptional performance and broad compatibility with membrane (Western blot) and 96-well plate (ELISA) assays.
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Blocker™ Blocking Buffer, Casein
Supplier: Thermo Fisher Scientific
Blocker™ Casein in PBS or TBS are ready-to-use, solutions of purified casein protein for blocking steps in Western blot, ELISA, IHC and nucleic acid detection methods.
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Tris-Borate-EDTA (TBE) buffer, 10X, Molecular biology grade
Supplier: PanReac AppliChem
10x konzentrierte Lösung aus 0,89 M Tris, 0,89 M Borsäure und 0,02 M EDTA-Na2, pH 8,3 ±0,2.
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Proteometer-L Consumables Kit (800 Titer)
Supplier: NOVILYTIC
800 Titer + aggregate assays. Consumables only, reactor not included. Designed for customers who are reordering. Use with any LC. For human mAbs, multi-specifics, recombinant proteins.
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TE Buffers 100X
Supplier: G-Biosciences
TE Buffer (Tris-EDTA Buffer) is a common molecular biology buffer that is used to protect DNA and RNA from degradation. The Tris buffering agent and EDTA metal chelating properties help protect DNA and RNA.
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Phosphate-buffered saline (PBS), Corning®
Supplier: Corning
Phosphate buffered saline (PBS) is a buffer solution containing sodium chloride, sodium phosphate, and in some formulations potassium chloride and potassium phosphate
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Proteometer-L starter kit reagent part 2/2 (200 assays)
Supplier: NOVILYTIC
Fluoreszenzreagenzien für das Proteometer-L Starterkit. Wegen Transportbedingungen wird es separat verkauft.
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Blocking buffers, protein-free, Pierce™
Supplier: Thermo Fisher Scientific
Pierce Protein-free blocking buffer is a non-protein compound that provides effective blocking for membrane-based and plate-based protein detection methods, resulting in extremely low background. It is supplied in a convenient, ready-to-use format.
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Blocking buffer, dry blend, SuperBlock™
Supplier: Thermo Fisher Scientific
SuperBlock (TBS) Blocking Buffer is an optimised TBS solution containing a protein that provides incredibly fast and effective blocking for ELISA, IHC and Western blot analysis.
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Blocking buffer, concentrate, Pierce™ Clear Milk
Supplier: Thermo Fisher Scientific
This clear milk blocking buffer is a long shelf life, high performance replacement for homemade milk blocking buffers in Western blotting applications.
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Pierce™ BupH™ Wash Buffers, Dry Blend Pack
Supplier: Thermo Fisher Scientific
BupH packs are pre-blended and pre-measured dry mixtures of buffers that are easy to prepare and used in protein purification and common laboratory techniques.
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North2South™ Hybridisation Buffer
Supplier: Thermo Fisher Scientific
North2South™ hybridisation buffer is an aqueous buffer which is used with the North2South™ chemiluminescent detection kit.
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Wash buffer, Pierce™
Supplier: Thermo Fisher Scientific
These wash buffers are designed for use in dialysis, cross-linking, enzyme assays, ELISAs, immunohistochemistry, protein plate-coating, biotinylation applications.
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Pierce™ Lane Marker Non-reducing Sample Buffer (5X)
Supplier: Thermo Fisher Scientific
Pierce™ Lane Marker Non-Reducing Sample Buffer is a ready-to-use 5X SDS-PAGE sample loading buffer in a non-reducing formulation, with a pink dye buffer-front marker.
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Waschpuffer, femto TBST™ und femto PBST™
Supplier: G-Biosciences
Die Waschpuffer femto TBST™ und PBST™ erhöhen die Empfindlichkeit von Immunassays durch Minimierung des „Auswaschens“ von Immunagenten oder Immunkomplexen, einem Problem, das häufig im Zusammenhang mit klassischen TBST- und PBST-Puffern auftritt. Die Waschpuffer unterstützen das Erstellen sauberer Hintergründe und führen damit zu einem besseren Verhältnis von Signal und Hintergrund. Sie können bei Inkubations- und Waschvorgängen verwendet werden.
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Wash buffer, Pierce™ Fast Wash Buffer
Supplier: Thermo Fisher Scientific
Pierce™ Fast Wash buffer, 10X, for use with Pierce™ Fast Blocking buffer.
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TAE buffer, 50X
Supplier: EDVOTEK
This 50X concentrated solution (Tris-acetate, EDTA, pH 7,8) is sufficient for making 5 L of diluted working buffer for making agarose gels and electrophoresis chamber buffer.
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VHB DNA Washing Buffer
Supplier: OMEGA BIO-TEK
VHB buffer must be diluted with ethanol before use.
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ReadiUse™ Yeast Cell Lysis Buffer, 5X
Supplier: AAT Bioquest
ReadiUse™ Yeast Cell Lysis Buffer is a biochemical reagent designed for efficient yeast cell lysis and protein solubilisation in laboratory applications.
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ReadiUse™ Bacterial Cell Lysis Buffer *5X* (10 ml)
Supplier: AAT Bioquest
Cell lysis refers to the breaking down of cells, and it is often used to analyze specific cellular compositions such as proteins, lipids, nucleic acids, reporter molecules, cell signal molecules and other small biomolecules.
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Buffer solutions, colour coded, Fluka™
Supplier: Honeywell Chemicals
These buffer solutions are colour-coded for easy identification and the pH 4 and 7 version have fungicide additive.
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Puffer, TBS 20X-Lösung, pH 7,4, molekularbiologischer Grad, ultrarein
Supplier: Thermo Fisher Scientific
Verwendung in Western Blotting Protokollen.
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Buffer solution pH 4, citrate buffer
Supplier: Thermo Fisher Scientific
pH Buffer solution, Citrat, pH-Wert: 4,00, ±0,02, Farblos
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Buffer solutions, according to DIN 19266, Fluka™
Supplier: Honeywell Chemicals
Buffers with fungicide (except pH 10,012)