Zellkultur-Ergänzungen und -Zusätze

Supplier: VWR Chemicals

Eigelb-Tellurit-Emulsion, VWR®

Supplier: VWR Chemicals

Beinhaltet eine Mischung von Protease-Inhibitoren: AEBSF, Aprotinin, E-64, Bestatin und Leupeptin. Jedes Gefäß kann in 1 ml deionisiertem Wasser zu einer 100X Lösung wiederhergestellt werden.

Supplier: VWR Chemicals

Der wasserlösliche Teil der autolysierten Hefe enthält einen Vitamin-B-Komplex.

Supplier: VWR Chemicals

VWR® Trypsin-EDTA solutions are used in cell culture to remove adherent cells from a culture surface. Trypsin is a porcine pancreas-derived enzyme that is commonly used for the dissociation and disaggregation of anchorage-dependent mammalian cells and tissues. The concentration of trypsin necessary to separate cells from their substrate is dependent primarily on the cell type and the age of the culture. EDTA, a chelating agent, enhances enzymatic activity by removing calcium and magnesium ions. These ions obscure the peptide bonds on which trypsin acts as well as enhancing cell to cell adhesion.

Supplier: Biowest

L-Glutamin ist eine essentielle Aminosäure, die von praktisch allen in Kultur gezüchteten Säugetier- und Insektenzellen benötigt wird. Es ist ein entscheidender Bestandteil vieler Zellkulturmedien und dient als wichtige Energiequelle für Zellen in Kultur. L-Glutamin ist als Trockenpulver und als gefrorene Lösung sehr stabil. In flüssigen Medien oder Stammlösungen kann L-Glutamin jedoch relativ schnell abgebaut werden. L-Glutamin ist labiler in Zellkulturlösungen als andere Aminosäuren.

Supplier: Abcam

Microsome Isolation Kit (ab206995) provides a convenient and fast way to isolate microsomal fractions from animal tissues for downstream applications such as assessing CYP-mediated drug metabolism and xenobiotic biotransformation, and protein profiling of microsomal membrane proteins by SDS-PAGE and western blotting.

Supplier: Enzo Life Sciences

Natural products are an unsurpassed source of chemical diversity and an ideal starting point for any screening program for pharmacologically active small molecules. Historically, natural products have been the most successful source of new drugs. The Screen-Well® natural product library offers over 500 isolated natural compounds of known structure and includes many compounds from ethnopharmacology. Compounds are provided in solution in DMSO. Individual compounds or subsets can be resupplied in gram quantities.

Supplier: HyClone products (Cytiva)

With HyrTryp™ cell dissociation reagent, an animal-derived component-free (ADCF) recombinant alternative to porcine trypsin, you can dissociate and harvest cells with confidence. Simply substitute it in your existing protocol.

Supplier: Abcam

Phosphatase Inhibitor Cocktail I (ab201112) is part of the multiplex kits range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.

Supplier: HyClone products (Cytiva)

HyClone™ L-glutamine is used as a supplement in serum- and protein-free media. It supports the growth of cells that have high energy demands and synthesise large amounts of proteins and nucleic acids.

Supplier: G-Biosciences

The PhosphataseArrest™ phosphatase inhibitor cocktails are ready to use 100X solutions that are simply added to either extraction buffers or samples.

Supplier: G-Biosciences

A broad range, 100X concentrated, ready-to-use protease inhibitor cocktail, Plant ProteaseArrest™ inhibits plant serine, cysteine and other plant specific proteases including aminopeptidases, aspartic and metalloproteases.

Supplier: G-Biosciences

Protease-PhosphataseArrest™ provides full protection of protein samples from proteases and phosphatases released during the preparation of cell and tissue lysates. Protease-PhosphataseArrest™ contains both irreversible and reversible protease inhibitors to inhibit serine, cysteine and other proteases along with five phosphatase inhibitors that target all the phosphatase categories: serine/threonine (Ser/Thr) specific, tyrosine specific and dual specificity phosphatases.

Supplier: G-Biosciences

A ready to use, 100X concentrated, broad range protease inhibitor cocktail that is fully compatible with 2D electrophoresis and subsequent mass spectrometry.

Supplier: G-Biosciences

Protease inhibitor set contains 12 ready to use individual protease inhibitors, each at a 100X concentration. Various concentrations and/or combinations of protease inhibitors may be used to inhibit a broad spectrum of protease activity. The Protease inhibitor set can be used to design specific protease inhibitor cocktails, supplement existing cocktails or to screen for specific protease classes.

Supplier: PROTIDE PHARMACEUTICALS

Serum replacement with similar properties to TCM™ but contains additional components for cell types that have required higher concentrations of serum (typically more than 10%). TM-235™ is defined and multi-purpose. Contains highly purified, heat-treated bovine serum albumin, heat-treated bovine transferrin and recombinant insulin.