"western blot detection systems"

Supplier: G-Biosciences

G-Biosciences' SWIFT™ Western Diluent is a unique solution that reduces the blocking and antibody incubations on Western blot membranes from >4 hours to <60 minutes

Supplier: Thermo Scientific Chemicals

For chemiluminecent detection of HRP-based Western blotting systems.

Supplier: Cytiva

Amersham Hybond LFP 0.2 PVDF is a low-fluorescent, hydrophobic membrane, with 0.2 µm pore size, for protein transfer. Amersham Hybond LFP is optimized for fluorescent detection in Western blotting applications and for use with the Amersham ECL Plex detection system.

Supplier: Rockland Immunochemical

. This Antibody Has Been Tested For Use In Elisa And Western Blotting Can Be Used To Detect Blt (14-Residue) In Recombinant Expression Systems. Blt Is The Minimal Substrate Requirement Of Bira-Catalyzed Biotinylation.

Supplier: Cytiva

Hybond™ ECL™ gives excellent sensitivity, resolution, and low background for all labelling and detection systems (radioactive, non radioactive, and chromogenic), especially when used with ECL™, ECL™ Prime, and ECL™ Plex Western Blotting Detection kits and reagents. Special packaging features include interleaf on both sides of the membrane and air-tight resealable aluminium bag with label space for marking the 'opened on' date.

Supplier: Cytiva

Amersham™ ECL™ Plex™ fluorescent Rainbow™ markers are protein molecular weight markers optimised for use with the ECL™ Plex™ Western blotting detection system. They are designed to provide visible marker bands on gels and membranes, as well as fluorescent images using Cy3 and Cy5 channels.

Supplier: Cytiva

Amersham™ ECL™ Prime is a highly sensitive Western blotting detection reagent characterised by stable signal emission, allowing for the possibility of repeated exposures and making it easier to process several blots in the same experimental run. Amersham™ ECL™ Prime delivers a detection system that is sensitive, stable, precisely quantitative across a wide dynamic range of protein levels, and conservative in its consumption of expensive antibody reagents. The high performance of Amersham™ ECL™ Prime is due to the presence of an enhancer in the reagent, increasing enzyme turnover, and thereby significantly increasing both signal intensity and duration. The light signal is further intensified by the addition of a catalyst.

Supplier: G-Biosciences

The femtoLUCENT™ PLUS Reagent Kit facilitates the quick and sensitive non-isotopic detection of proteins on transfer membranes.

Supplier: Thermo Scientific

This Antibody has been successfully used in Western blot with human and mouse samples. Western blot analysis of MA1-014-HRP specifically detects SOX2 protein at ~36 kDa in the nucleus of embryonal carcinoma cells. This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT).

Supplier: Proteintech

DNM3 (dynamin 3) is a member of dynamin proteins that participate in membrane rearrangements such as vesicular trafficking and phaocytosis. Three dynamin proteins encoded by different genes are present in mammals: DNM1 is mainly expressed in central nervous system, DNM2 ubiquitously expressed, and DNM3 expressed chiefly in the testis and brain. Western blot using this antibody specifically detected the 100 kDa DNM3 in mouse brain. It doesn't cross-react with other DNMs. (22977254, 21671749)

Supplier: Abcam

Anti-GFP antibody ab13970 is a chicken polyclonal antibody that is used to detect GFP in western blotting and immunofluorescence. Noted for increased sensitivity and decreased background, this GFP chicken polyclonal antibody is ideal for human, mouse, rat, and other mammalian studies. Immunoglobulin IgY is the major antibody produced by chickens and it does not activate the human complement system. - increase sensitivity for mammalian applications - decreased background signal - most highly cited GFP antibody cited >4700 times.

Supplier: Genetex

Trident Plus Western HRP substrate is a luminol-based, chemiluminescent substrate for the detection of HRP-conjugated antibody on immunoblots at picogram level. It produces an excellent signal that can be easily detected by chemiluminescent imaging methods including X-ray film and chemiluminescence imaging system.

Supplier: Cytiva

The Amersham™ QuickStain Kit contains a Cy™5 fluorophore and labeling buffer for easy detection of proteins in SDSPAGE gels and on Western blotting membranes. The ready to use Cy5 N-hydroxysuccinimide (NHS) ester and Tris labeling buffer ensure robust and consistent labeling for detection of proteins in diverse samples.

Supplier: Proteintech

The M13 family has seven members, including ECEL1 (endothelin-converting enzyme-like 1), one of the newest members. ECEL1 is expressed predominantly in the central nervous system. It has been proposed that the enzyme has a role in the nervous regulation of the respiratory system.It may contribute to the degradation of peptide hormones and be involved in the inactivation of neuronal peptides.It has 2 isoforms produced by alternative splicing and 3 glycosylation sites.It can be detected a band of 95kDa in CHO cells by western blot because of the heavily glycosylated.

Supplier: Biotium

In Western blotting, this antibody detects an antigen of 125 kDa in human liver and 135 kDa in tumors of histiocytic origin. Comparative study of this MAb and a standard CD68 MAb showed that their antigens are different. Its antigen in all macrophage types studied is located on the plasma membrane and within cytoplasmic structures including lysosomes. This MAb shows a restricted reactivity to cells of the monocyte/macrophage system. It specifically reacts with blood monocytes and stains resident macrophages in a wide variety of human tissues. This MAb does not stain antigen-presenting cells, e.g., Langerhans cells. Reportedly, its reactivity is restricted to histiocytes and macrophages.

Supplier: Biotium

In Western blotting, this antibody detects an antigen of 125 kDa in human liver and 135 kDa in tumors of histiocytic origin. Comparative study of this MAb and a standard CD68 MAb showed that their antigens are different. Its antigen in all macrophage types studied is located on the plasma membrane and within cytoplasmic structures including lysosomes. This MAb shows a restricted reactivity to cells of the monocyte/macrophage system. It specifically reacts with blood monocytes and stains resident macrophages in a wide variety of human tissues. This MAb does not stain antigen-presenting cells, e.g., Langerhans cells. Reportedly, its reactivity is restricted to histiocytes and macrophages.