"protein purification system"

Supplier: IBI Scientific

IBI Isolate is a phenol, chloroform, and guanidine isothiocyanate based scalable solution for extracting high-quality total RNA as well as simultaneous extraction of RNA, DNA, and protein from a wide variety of samples.

Supplier: Cytiva

Sepharose™ CL-4B is a cross-linked agarose gel filtration base matrix and is frequently used for coupling affinity ligands to the matrix.

Supplier: Cytiva

Sephadex ion exchangers are produced by introducing functional groups onto the cross-linked dextran matrix.

Supplier: Cytiva

Superdex prep grade is a preparative gel filtration medium with a composite matrix of dextran and agarose

Supplier: Abcam

HRV14 3C (PreScission) Protease Agarose Beads, ab286883, (previously known as #M1029) is prepared by covalent coupling active HRV14 3C Protease to activated 4% cross-linked agarose beads.

Supplier: Cytiva

EAH Sepharose™ 4B allows for the stable carbodiimide-based coupling of carboxyl groups to Sepharose® 4B via an 11-atom hydrophilic spacer arm that permits extremely stable coupling.

Supplier: Cytiva

Sephadex ion exchangers are produced by introducing functional groups onto the cross-linked dextran matrix.

Supplier: Abcam

Protein A Magnetic Beads (ab214286) is part of the reagents, controls & accessories range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.

Supplier: Cytiva

Butyl Sepharose™ HP media is particularly well suited for intermediate purification and polishing steps providing high resolution due to the small particle size.

Supplier: Cytiva

Sephadex G-15 is a well established gel filtration medium for desalting and buffer exchange of peptides and small biomolecules >1500 molecular weight.

Supplier: Proteintech

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. Maltose binding protein (MBP) is the 370 amino acid product of the E.coli mal E gene. MBP is a useful affinity tag that can increase the expression level and solubility of the resulting tagged protein. The MBP tag also promotes proper folding of the attached protein. Plasmid vectors have been constructed utilizing the MBP domain that allow the synthesis of high levels of MBP-fusion proteins that can be purified in a one step procedure by affinity chromatography cross linked amylose resin. Once bound to amylose, the MBP protein can then be separated from the target protein by cleavage by coagulation Factor Xa at a specific four residue site. Alternatively, the intact fusion protein can be specifically eluted from the resin by the addition of excess free maltose. Subsequent to elution, MBP fusion protein can be visualized either by western blot analysis or immunoprecipitation using antibodies specific for the MBP-tag. This antibody recognizes MBP (Maltose binding protein) TAG in some expression systems.

Supplier: Thermo Scientific

AminoLink™ Plus Coupling Resin allows simple and efficient covalent immobilization of proteins to a beaded agarose support, providing a valuable tool for affinity purification of antibodies, antigens or other biomolecules.

Supplier: Cytiva

Sephadex G-75 is well established gel filtration medium for desalting and buffer exchange of large biomolecules >80 000 molecular weight.

Supplier: Thermo Scientific

Thermo Scientific Pierce Glutathione Superflow Agarose consists of glutathione that has been immobilized to a crosslinked 6% beaded agarose that is designed for high-purity, large-scale purification of GST-tagged proteins.

Supplier: Cytiva

MabSelect™ PrismA has been improved with an optimised high-flow agarose base matrix and a genetically engineered protein A-derived ligand, allowing future demands in mAb processing to be met.

Supplier: Thermo Scientific

Thermo Scientific Pierce CDI-Activated Agarose is carbonyldiimidazole affinity chromatography resin, activated for covalent immobilization of N-nucleophiles and primary amine ligands in aqueous or organic solvent conditions.