buffers

Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.

Fluka™ Fluoride buffer solution According to DEV D4, Honeywell Research Chemicals

Supplier: Honeywell Research Chemicals

TISAB according to DEV D4.

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Buffer, Sodium Acetate Buffer Solution pH 5,2 (3 mol/L), ROCK

Supplier: Rockland Immunochemical

3.0 M Sodium Acetate, pH 5.2

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HyClone™ HEPES Buffer Solution 1 M, HyClone products (Cytiva)

Supplier: Cytiva

Maintain physiological pH in cell culture, despite changes in carbon dioxide concentration (produced by cellular respiration) with Cytiva HyClone HEPES solution.

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NEBuffer r2.1, New England Biolabs

Supplier: New England Biolabs (NEB)

New England Biolabs provides a color-coded 10 X NEBuffer with each restriction endonuclease to ensure optimal (100%) activity. Most of our enzymes are supplied with one of four standard NEBuffers. These buffers contain Recombinant Albumin (NEB #B9200).

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EDTA, pH 8.0, 0.5M

Supplier: GROWCELLS

Ethylenediaminetetraacetic acid is a polyamino carboxylic acid, commonly used to deactivate metal dependent enzymes, or as a chelating agent that binds to metal ions

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SDS Protein Gel Loading Solution, 2X, Quality Biological

Supplier: QUALITY BIOLOGICAL, INC.

"SDS Protein Gel Loading Solution, 2X is formulated at the traditional 2X stocks, for standard volume of sample to be loaded per well. Simply load the gel sample wells and visualize progress of the electrophoresis run by monitoring the location of the blue dye front.

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HyClone™ AdvanceSTEM, HEPES Buffer, ES Qualified, HyClone products (Cytiva)

Supplier: Cytiva

HyClone AdvanceSTEM ES Qualified HEPES provides maintenance of physiological pH in cell culture despite changes in carbon dioxide.

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Buffer, EDTA Buffer Solution pH 8,5 (0,5 mol/L)

Supplier: G-Biosciences

A DNase and RNase free solution of 0.5M EDTA.

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G-Biosciences Sterile Dulbecco's Phosphate Buffered Saline with Magnesium and Calcium

Supplier: G-Biosciences

Dulbecco’s phosphate buffered saline with magnesium and calcium is used to provide buffer system to maintain cell culture in the physiological pH range (7 to 7.6).

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Blocking Solution, Quality Biological

Supplier: QUALITY BIOLOGICAL, INC.

Blocking is a very important step of western blotting, as it prevents antibodies from binding to the membrane nonspecifically. Blocking is often made with 5% BSA or nonfat dried milk to reduce the background. Nonfat dried milk is often preferred as it is inexpensive and widely available. However, milk proteins are not compatible with all detection labels, so care must be taken to choose the appropriate blocking solution.