buffers
Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.
Saline Solutions, NERL Diagnostics
Supplier: Thermo Fisher Scientific
Blood bank saline pH 7.0–7.2 is a phosphate buffered saline solution manufactured to match the pH of human blood
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ZymoBIOMICS Lysis Solution, Zymo Research
Supplier: Zymo Research
ZymoBIOMICS Lysis Solution is designed to be used with the ZymoBIOMICS DNA extraction kits. Used in combination with the ZR BashingBead Lysis Tubes, it assists with microbial lysis. It is certified to be low-bioburden to ensure minimal background contaminants for low abundance microbiome samples.
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NEBuffer™ Set (r1.1, r2.1, r3.1 and rCutSmart™), New England Biolabs
Supplier: New England Biolabs (NEB)
New England Biolabs provides 10X NEBuffer with each restriction endonuclease to assure optimal (100%) activity. Most of our enzymes are supplied with one of four standard NEBuffers. This set contains NEBuffers that contain recombinant Albumin.
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Customized SDS Protein Gel Loading Solution, Quality Biological
Supplier: QUALITY BIOLOGICAL, INC.
This is a special formulation of loading solution for protein gel electrophoresis.
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Sodium Acetate, 3 M Aqueous Solution, pH 7.0, RNAse Free, Thermo Scientific
Supplier: Thermo Scientific Chemicals
Liquid
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PBS (Phosphate Buffered Saline), 1X, with EDTA, pH 7.4, Teknova
Supplier: Teknova
PBS buffer contains 137 mM sodium chloride, 7 mM sodium phosphate dibasic, 3 mM potassium chloride, 1.4 mM potassium phosphate monobasic, 4 mM EDTA pH 7.4
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pH 10 Buffer Solution
Supplier: Hanna
High-quality pH 10 calibration buffer for standardizing meters in basic solutions.
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SDS-Page Running Gel Buffers, 10X Concentrate
Supplier: Rockland Immunochemical
SDS-PAGE, sodium dodecyl sulfate polyacrylamide gel electrophoresis, describes a technique widely used in biochemistry to separate proteins according to their electrophoretic mobility (a function of the length of a polypeptide chain and its charge) and no other physical feature
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Magnesium Sulfate (MgSO₄) Solution, New England Biolabs
Supplier: New England Biolabs (NEB)
100 mM MgSO₄ for use in conjunction with polymerase reaction buffers.
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FirstChoice™ Blocking Agents, G-Biosciences
Supplier: G-Biosciences
G-Biosciences' FirstChoice™ Blocking Buffer is ideal as a first choice for optimization of new assays and systems, or when determining the optimal blocking buffer for elimination of non-specific binding sites in ELISA, blotting, immunohistochemistry and other applications.
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EDTA (0.5 M), pH 8.0, Quality Biological
Supplier: QUALITY BIOLOGICAL, INC.
EDTA (Ethylenediaminetetraacetic acid), a metal chelator, is widely used in molecular biology as a chemical reagent to reduce nuclease activity (i.e. metalloenzyme). EDTA solution can be used to study cell biology, molecular biology, bioactive small molecules, biochemicals, solutions and reagents, inorganic salts, and chelators.
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Dulbecco's Phosphate-Buffered Saline (DPBS)
Supplier: Promocell
These Dulbecco's PBS solution are used in tissue culture, cell isolation procedures and molecular biology.
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Nuclear Extraction Kit, Abcam
Supplier: Abcam
Nuclear extraction kit provides a simple and selective method for nuclear protein extraction and fractionation from cells or tissue samples in just 1 hour. This kit includes all necessary reagents to obtain high yields of nuclear proteins suitable for various downstream applications. - Cited in over 255 publications - High retention of enzyme activity – contains 80% less salt than conventional kits and no SDS, allowing better enzyme activity retention - Suitable for western blotting, protein-DNA binding assays, nuclear enzyme assays, and more - Fast, simple procedure that ensures optimized nuclear protein isolation
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Pierce™ Subcellular Protein Fractionation Kit for Tissues, Thermo Scientific
Supplier: Thermo Scientific
The Pierce™ kit is ideal for cell fractionation and enrichment of proteins from five different cellular compartments within two hours.
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Buffer, TAE solution 50X concentrate (TRIS-acetate-EDTA buffer)
Supplier: Rockland Immunochemical
50X Tris-Acetate-EDTA (TAE) (2.0 M Tris-Acetate, 0.05 M EDTA, pH 8.3)
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Buffer, Sodium Acetate Buffer Solution pH 5,2 (3 mol/L), ROCK
Supplier: Rockland Immunochemical
3.0 M Sodium Acetate, pH 5.2
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