buffers
Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.
SSPE Buffer, 20X concentrate, pH 7.4, Rockland Immunochemicals
Supplier: Rockland Immunochemical
20X SSPE buffer (3.0 M Sodium Chloride, 0.2 M Sodium Hydrogen Phosphate, 0.02 M EDTA, pH 7.4) - DEPC treated.
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HeLa Nuclear Extract, Abcam
Supplier: Abcam
Nuclear extract is prepared from HeLa cells, which contains a variety of DNA-binding proteins, transcription factors, and other nuclear proteins.
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DNA/RNA Shield™ Lysis Tubes, Zymo Research
Supplier: Zymo Research
Collect and inactivate samples in lysis tubes prefilled with DNA/RNA Shield™.
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Pierce Subcellular Protein Fractionation Kit, Thermo Scientific
Supplier: Thermo Scientific
Thermo Scientific Subcellular Protein Fractionation Kit for Cultured Cells enables segregation and enrichment of proteins from five different cellular compartments.
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SDS Protein Gel Loading Solution, 5X, Quality Biological
Supplier: QUALITY BIOLOGICAL, INC.
Formulated as 5X rather than the traditional 2X stocks, SDS Protein Gel Loading Solution 5X enables a larger volume of sample to be loaded per well.
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Sodium acetate, 3 M aq. soln., pH 7.0, autoclaved, Thermo Scientific
Supplier: Thermo Scientific Chemicals
Liquid
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Corning® 10% SDS (Sodium Dodecyl Sulfate), Corning
Supplier: Corning
Sodium Dodecyl Sulfate (SDS) is an anionic detergent with applications in SDS-PAGE protein analysis and nucleic acid purification. It is a wetting agent that is effective in both acid and alkaline solutions, and is most often used as a protein and lipid solubilization reagent. SDS is also a powerful protein denaturant. This 10% solution is prepared in 18 megaohm water and is 0.2µm filtered.
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Nuclear (Nucleic Acid-Free) Extraction Kit, Abcam
Supplier: Abcam
Nuclear (Nucleic Acid-Free) Extraction Kit (ab113477) provides a simple and selective method for extracting nuclear proteins (nucleic-acid free) in just 1 hour for a variety of applications such as western blotting, protein-DNA binding assays, or any other procedures requiring optimized nucleic acid-free nuclear proteins.
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Hanna pH Buffer Sachets
Supplier: Hanna
This buffer value is widely used in water purification plants, in the food industry, and wherever the pH is expected to be slightly acidic.
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Human TruStain FcX™, Fc Receptor Blocking Solution, BioLegend
Supplier: Biolegend
Human Fc receptors (FcRs) are expressed on a variety of cells, such as monocytes, granulocytes, B cells and dendritic cells
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Ammonia Ionic Strength Adjustor (ISA) Solution
Supplier: Hach
Ammonia Ionic Strength Adjustor (ISA) Buffer Solution for ammonia determinations by electrode methods.
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Immunoassay Blocking (BSA Free)
Supplier: Abcam
Immunoassay Blocking (BSA Free) (ab171535) maintains the activity of antibodies and other biomolecules adsorbed or immobilized to solid substrates.
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Acetate Buffer, pH 4.0, for Residual Chlorine Analysis, Ricca Chemical Company
Supplier: Ricca Chemical
Suitable for use in the following compendial methods: -APHA 4500-ClO C, Reagent 3, for Chlorine Dioxide via Amperometric Method I -APHA 4500-Cl E, Reagent 3d, for Chlorine (Residual) via Low-Level Amperometric Titration Method -APHA 4500-Cl D, Reagent 3d, for Chlorine (Residual) via Amperometric Titration Method -APHA 4500-Cl C, Reagent 3e, for Chlorine (Residual) via Iodometric Method II -EPA 330.2, Reagent 5.2 for Chlorine, Total Residual -EPA 330.2, Reagent 5.5 for Chlorine, Total Residual -ASTM D1253, Reagent 8.3 for Residual Chlorine
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Buffer, TE Solution (TRIS-EDTA Buffer) pH 8.0
Supplier: Rockland Immunochemical
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ZymoBIOMICS Lysis Solution, Zymo Research
Supplier: Zymo Research
ZymoBIOMICS Lysis Solution is designed to be used with the ZymoBIOMICS DNA extraction kits. Used in combination with the ZR BashingBead Lysis Tubes, it assists with microbial lysis. It is certified to be low-bioburden to ensure minimal background contaminants for low abundance microbiome samples.
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NEBuffer™ Set (r1.1, r2.1, r3.1 and rCutSmart™), New England Biolabs
Supplier: New England Biolabs (NEB)
New England Biolabs provides 10X NEBuffer with each restriction endonuclease to assure optimal (100%) activity. Most of our enzymes are supplied with one of four standard NEBuffers. This set contains NEBuffers that contain recombinant Albumin.
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