buffers

Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.

VWR® TRIS Glycine Buffer

Supplier: VWR

Following electrophoresis, proteins are frequently transferred from the polyacrylamide gel onto PVDF or nitrocellulose membranes for use in immunoblotting or sequencing

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VWR® EDTA 0.5M, Biotechnology Grade

Supplier: VWR

Cloning Buffer, EDTA, 0.5M Solution, pH 8.0

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Environmental Express® Oakton® CERTIFIED CRM One-Shot™ Buffer Solution Kit

Supplier: VWR International

Environmental Express Oakton pH buffers are Certified Reference Materials, making them one of the most accurate solutions available.

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VWR® Sodium Acetate, 3M Solution, pH 5.2, Biotechnology Grade

Supplier: VWR

Cloning Buffer, Sodium acetate solution 3M, pH 5.2

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VWR® TRIS Glycine Buffer, 10X Liquid Concentrate

Supplier: VWR

A 1X working solution contains 0.025M Tris base and 0.192 M Glycine.

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Macron Fine Chemicals™ BuffAR®, pH Buffer Solutions

Supplier: AVANTOR PERFORMANCE MATERIAL LLC

Accurate to within ±0.01 pH at 25 °C (±0.02 for pH 9 and 9.18).

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Tris-Gly-SDS

Supplier: Ward's Science

Tris-Gly-SDS Buffer Solution, Premixed Powder, Makes 6 L

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VWR® EDTA 0.5M, Biotechnology Grade

Supplier: VWR

Cloning Buffer, EDTA, 0.5M Solution, pH 8.0

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VWR® MOPS Buffer Solution 10X, Biotechnology Grade

Supplier: VWR

Optimized for RNA Agarose Electrophoresis

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EDTA, Standard Solution, 0.5M, VWR Chemicals BDH®

Supplier: VWR International

N.I.S.T traceable to SRM 919.

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Titripur® Ammonium Buffer Solutions for Complexometry (Ammonium Chloride/Ammonia), MilliporeSigma

Supplier: MilliporeSigma

TitriPUR® Ammonium Buffer Solution, Titripur®

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Chemi Blot Blocking Buffer, Azure

Supplier: Azure Biosystems

Azure Chemi Blot Blocking Buffer, Ready-to-use block that enhances chemiluminescent signal, for use with chemiluminescent Western blots to enhance signal and lower background. Size: 500mL

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Titrisol® Buffer Concentrate (Glycine/Hydrochloric acid), Traceable to SRM of NIST and PTB, MilliporeSigma

Supplier: MilliporeSigma

Titrisol* (glycine/hydrochloric acid), traceable to SRM of NIST and PTB for 500 ml buffer solution, pH 1.00 +/- 0.02 (20 degree C), Synonym Titrisols for preparation of buffer solutions, Hazard May be corrosive to metal.

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VWR® Phosphate Buffered Saline (PBS), Powdered, Ultra Pure Grade

Supplier: VWR

Preparation of PBS using 10 L and 50 L Format:
Dissolve 9.88 g of PBS powder in 1 L of water to prepare a 1X PBS solution containing 137 mM sodium chloride, 2.7 mM potassium chloride and 9.8 mM phosphate buffer.
The 10 L and 50 L pack sizes contain sufficient powder to prepare 10 L and 50 L of 1X PBS, respectively.