"USLP070318BIOP00135"
EnGen® sgRNA Synthesis Kits, S. pyogenes, New England Biolabs
Supplier: New England Biolabs (NEB)
In nature, S. pyogenes Cas9 is programmed with two separate RNAs, the crRNA and tracrRNA.
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EnGen® Spy Cas9 NLS, New England Biolabs
Supplier: New England Biolabs (NEB)
EnGen Spy Cas9 NLS, is an RNA-guided endonuclease that catalyzes site- specific cleavage of double stranded DNA. The location of the break is within the target sequence 3 bases from the NGG PAM (Protospacer Adjacent Motif) (1). The PAM sequence, NGG, must follow the targeted region on the opposite strand of the DNA with respect to the region complementary sgRNA sequence. EnGen Spy Cas9 NLS contains Simian virus 40 (SV40) T antigen nuclear localization sequence (NLS) on the N- and C- termini of the protein.
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Catalog #: CA21905-026
Supplier: Kimberly-Clark